| 著作名稱： | Chang TL*, Liou PS, Cheng PY, Chang HN, Tsai PJ. Borneol and luteolin from Chrysanthemum morifolium regulates ubiquitin-signal degradation. J Agric Food Chem. 2018 Aug 8;66(31):8280-8290 |
| 年度： | 2018 |
| 類別： |
期刊論文
J Agric Food Chem
|
| 摘要： | Targeting the two degradation systems, ubiquitin proteasome pathway and ubiquitin signal autophagy lysosome
system, plays an important function in cancer prevention. Borneol is called an “upper guiding drug”. Luteolin has demonstrated anticancer activity. The fact that borneol regulates luteolin can be sufficient to serve as an alternative strategy. Borneol activates luteolin to inhibit E1 and 20S activity (IC50 118.8 ± 15.7 μM) and perturb the 26S proteasome structure in vitro. Borneol regulates luteolin to inhibit 26S activity (IC50 157 ± 19 μM), induces apoptosis (LC50 134 ± 4 μM), and causes pre-G1 and G0/G1 arrest in HepG2 cells. Borneol regulates luteolin to induce ubiquitin signal autophagic degradation, resulting in induction of E1, reduction of USP47, and accumulation of p62 in HepG2 reporter cells. Interestingly, luteolin decreased Ub conjugates, while borneol increased the accumulation of Ub conjugates in HepG2 reporter cells. E1, p62, and ubiquitin levels were downregulated in borneol-treated HepG2 reporter cells at 24 h. These observations suggest a potential autophagic inhibitor of borneol that may guide luteolin in the ubiquitin proteasome pathway and the ubiquitin signal autophagic
degradation. |
| 關鍵字： | luteolin, borneol, proteasome, E1 enzyme, autophagy |
| 著作名稱： | Chang TL*, Chiang HY, Shen JY, Lin SW, Tsai PJ. Phenolic compounds stage an interplay between the ubiquitin–proteasome system and ubiquitin signal autophagic degradation for the ubiquitin-based cancer chemoprevention J Funct Foods 2015 August;17:857–71 |
| 年度： | 2015 |
| 類別： |
期刊論文
Journal of Functional Foods
|
| 摘要： | Inhibiting proteasome functional insufficiency plays an essential role in cancer prevention. Alternative strategies to target ubiquitin enzymes and ubiquitin-signal autophagic degradation can be the potential treatments. Phenolic compounds demonstrated anticancer effects. Phenolic compounds not only effectively inhibit E1, deubiquitinating enzyme, and 26S activity but also induce apoptosis in A549 cells. The IC50 of 26S activity and LD50 of A549 cells were nearly equivalent. Therefore, apoptosis may occur via the inhibition of the 26S proteasome activity. Inhibition of the proteasome by tannic acid in A549 cells resulted in reduction of S5a and 20S, accumulation of ubiquitin, and degradation of PARP, followed by induction of apoptosis. USP15 may play an essential role in the stability and activity of caspase-3. The levels of S5a, USP15 and USP47 were regulated by phenolic compounds, and the levels of procaspase-3 and polyADP-ribose polymerase were also decreased in A549 cells treated with phenolic compounds. Interestingly, tannic acid decreased Ubconjugates
while M + G + E increased the accumulation of Ub-conjugates in A549 reporter
cells, whereas ubiquitin, E1, and USP47 levels were low in M + G + E-treated A549 reporter cells at 24 hours. Taken together, phenolic compounds may play an important role in mediating apoptosis by regulating the ubiquitin–proteasome pathway and autophagy system. |
| 關鍵字： | |
| 著作名稱： | Lin YZ, Chang TL*, Chang CC* Electrochemical method for detecting ATP by 26S proteasome modified gold electrode Sens. Actuator B-Chem. 2014 Jan. 15;190(1):486-93 |
| 年度： | 2014 |
| 類別： |
期刊論文
Sens. Actuator B-Chem
|
| 摘要： | tElectrochemical detection for adenosine-5’-triphosphate (ATP) is developed using a 26S proteasomemodified gold electrode. In this method, the 26S proteasome is self-assembled onto the surface of agold electrode, forming a 26S-gold electrode probe which can detect ATP. When the 26S-gold electrodereacts with a solution without ATP, the 26S proteasome on the electrode surface separates into 20S pro-teasome and PA700/19S subcomplexes, the properties of surface charges of 26S-gold electrode inhibitingelectrolyte/electrode electron transmission. When ATP is present in the solution, the 26S proteasome self-assembles via ATP binding, the surface of 26S-gold electrode is turned into electroneutrality, and allowselectrolyte/electrode electron transmission in proportion to the amount of ATP present. This mechanismis tested for electrochemical detection of ATP, with experimental results showing that the redox currentschange with changing ATP concentrations in a linear fashion. ATP concentration can be determined overa range from 0.5 to 2.0 mM by linear sweep voltammetry. Moreover, the proposed method is successfullyapplied to the recognition and quantification of ATP in pig plasma |
| 關鍵字： | ATP, 26S proteasome |
| 著作名稱： | Chang TL*, Huang YH, Ou YD The role of ginsenosides in inhibiting ubiquitin-activating enzyme (E1) activity J Funct Foods 2014 March;7:462-70 |
| 年度： | 2014 |
| 類別： |
期刊論文
Journal of Functional Foods
|
| 摘要： | Functional foods targeting the ubiquitin-activating enzyme (E1) of the ubiquitin–proteasome
pathway as anti-tumour agents are considered to be important for cancer chemoprevention.
The low-toxicity Panax ginseng was used in traditional herbal medicine or food use
for either treating or preventing cancer for more than 1000 years. In this study, we constructed
an ubiquitin-tagged protein as a substrate to analyze the six ginsenosides Rb1,
Rb2, Rc, Rd, Re, and Rg1 by ubiquitination assay. More ubiquitinated E1 levels were induced
by increasing concentrations of ubiquitin-activating enzyme. Ginsenosides Re and Rg1
inhibited ubiquitin-activating enzyme. Unlike ginsenosides Re and Rg1, ginsenosides
Rb1, Rb2, Rc, and Rd increased ubiquitination on E1 enzyme. Interestingly, ginsenoside
Rg1 inhibits both the chymotrypsin-like activity of 26S proteasome and E1 activity. These
results suggest that ginsenoside Rg1 is a potential functional food application for cancer
prevention because of its specific E1 inhibitory effects. Furthermore, the 6-O-Glu position
of the ginsenoside may play a role in its E1 inhibitory property. Ginsenosides could be further
developed as a useful drug for cancer therapy. |
| 關鍵字： | Ginsenoside,Ubiquitin activating enzyme (E1), Ubiquitin |
| 著作名稱： | Chang TL*, Lin SW, Wu SL, Hong CM. Regulation of ubiquitin and 26S proteasome mediated by phenolic compounds during oxidative stress J Nutr Biochem 2013 Nov. 1;24(11):1970-81 |
| 年度： | 2013 |
| 類別： |
期刊論文
J Nutr Biochem
|
| 摘要： | Little attention has been devoted to studying the roles of natural antioxidants in the ubiquitin-proteasome pathway during oxidative stress. We demonstrated that a time course revealed that the reassociation of the 19S regulators with the 20S proteasomes occurred automatically and rapidly to reconstitute the 26S proteasomes, with up to 80% completion, within 5 minutes after H2O2 treatment. Ubiquitin, methyl gallate, and tannic acid are able to prevent H2O2 from inhibiting the 26S activity. We further show that the level of the ubiquitin, S5a, and 20S core subunits decreased within 30 mins, and increased after 24 h, of H2O2 treatment in Hep-2 cells. Phenolic compounds not only inhibited the 26S activity but also decreased the USP47 levels, which reduce the DNA damage repair rate during oxidative stress; in addition, the presence of DNA fragments, procaspase-3, and a decreased PARP also appeared as a result of the above conditions. Ubiquitin could serve as a protective substrate in H2O2 and phenolic compound-treated Hep-2 cells. Methyl gallate and tannic acid, as pro-oxidants, can attenuate the apoptotic response resulting from long-term oxidative stress. Collectively, these data demonstrate an important role for phenolic compounds in regulating the 26S proteasome and ubiquitin during oxidative stress. |
| 關鍵字： | |
| 著作名稱： | Chang TL*, Wang CH. Combination of quercetin and tannic acid in inhibiting 26S proteasome affects S5a and 20S expression, and accumulation of ubiquitin resulted in apoptosis in cancer chemoprevention. Biol Chem. 2013 Apr 1;394(4):561-75 |
| 年度： | 2013 |
| 類別： |
期刊論文
Biological Chemistry
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| 摘要： | To look for oral proteasome inhibitors, daily injested food is the best source for cancer chemoprevention.A combination of active components from vegetables,coffee, tea, and fruit could be more efficient to inhibit 26S proteasome activities for preventing cancer diseases. Tannic acid and quercetin have been shown to strongly inhibit 26S proteasome activity, but the molecular target involved remains unknown. Overlay assay,peptide assay, Western blot, and 2-D gels were used to assess the combination of quercetin and tannic acid as a potential inhibitor. Here, we demonstrated that the combination of quercetin and tannic acid (1) synergistically suppresses chymotrypsin-, caspase-, and trypsin-like proteolytic activities, (2) are tightly binding substrates,(3) do not perturb the proteasome structure, (4) inhibit the 26S proteasome affected by ubiquitin, ATP, or β-casein, and (5) inhibit β -casein degradation by the 26S proteasome in vitro . Finally, the inhibition of the proteasome by a combination of quercetin plus tannic acid in Hep-2 cells resulted in the induction of S5a at low dose, accumulation of ubiquitin, and the cleavage of procaspase-3, followed by the induction of apoptotic cell death. Evaluating the combination of quercetin and tannic acid as an oral drug to prevent cancer may provide a pharmacological rationale to pursue preclinical trials of this combination. |
| 關鍵字： | |
| 著作名稱： | Lin TY, Lee KY, Chang TL*, Chang CC*, Lin YZ. The electrochemical method for detecting 26S proteasome Sensors & Actuators: B. Chemical. 2011 Dec. 15;160(1):412-17 |
| 年度： | 2011 |
| 類別： |
期刊論文
Sensors & Actuators: B. Chemical.
|
| 摘要： | Detection of 26S proteasome, a multiproteolytic complex that degrades intracellular proteins in eukaryotic cells, by electrochemical methods is of interest for improved understanding of living cells and detection of cancer. This study develops an electrochemical system to detect 26S using a gold electrode modified by a self-assembled monolayer of 1,6-hexanedithiol (HDT) for capture of 26S proteasomes. When 26S is fixed on a HDT-gold electrode, it is found that electrolyte anions can enhance detection but also cause damage to the HDT layer. Cyclic voltammetry and electrochemical impedance spectroscopy demonstrate that HDT stability is better in LiClO4 solution than in sodium sulfate (Na2SO4) solution. Chymotrypsin-like activity of 26S as measured by fluorescence with Suc-LLVY-AMC substrate declines 10% with LiClO4 and 25% with Na2SO4. LiClO4 is a better electrolyte salt in a 26S-HDT-gold electrode application in Tris buffer. Increased electron transfer resistance is observed after binding 26S on the HDT-gold electrode. Stable 26S concentration is from 2 to 100 nM. As 26S concentration increases from 2 to 100 nM, the electron-transfer impedance of Fe(CN)64-/3- redox rises logarithmically. The range of electrochemical detection of 26S proteasome is nanomolar. |
| 關鍵字： | 26S proteasome; 1,6-hexanedithiol; Cyclic voltammetry; Electrochemical Impedance spectroscopy. |
| 著作名稱： | Chang TL*, Ding HY, Teng KN. Fang YC. 5,6,3,4-tetrahydroxy-7-methoxyflavone as a novel potential proteasome inhibitor. Planta Med. 2010 Jul;76(10):987-94 |
| 年度： | 2010 |
| 類別： |
期刊論文
Planta Medica
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| 摘要： | |
| 關鍵字： | |
| 著作名稱： | Chang TL* Inhibitory Effect of Flavonoids on 26S Proteasome Activity. J Agric Food Chem. 2009 Oct 21;57(20):9706-15. |
| 年度： | 2009 |
| 類別： |
期刊論文
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| 摘要： | |
| 關鍵字： | |
| 著作名稱： | Chang TL*, Chang CJ, Lee WY, Lin MN, Huang YW, Fan K. The roles of ubiquitin and 26S proteasome in human obesity. Metabolism-clinical and experimental 2009 Nov;58(11):1643-8. |
| 年度： | 2009 |
| 類別： |
期刊論文
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| 摘要： | |
| 關鍵字： | |
| 著作名稱： | Chang TL*, Lin MN, Chang CJ, Lee WY, Huang YW, Fan K. 2008.The roles of ubiquitin and 26S proteasome in human obesity. Obesity. 2008 16 (Supplement 1s): S207 |
| 年度： | 2008 |
| 類別： |
期刊論文
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| 摘要： | |
| 關鍵字： | |
| 著作名稱： | Chang TL*, Ding HY, Kao YW.2008. The role of ginsenoside Rd in inhibiting of 26S proteasome activity. J Agric Food Chem. 2008 Dec 24;56(24):12011-5 |
| 年度： | 2008 |
| 類別： |
期刊論文
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| 摘要： | |
| 關鍵字： | |
| 著作名稱： | Lee WY, Huang SC, Tzeng CC, Chang TL, Hsu KF. 2007. Alterations of metastasis-related genes identified using an oligonucleotide microarray of genistein-treated HCC1395 breast cancer cells. Nutr Cancer.58(2):239-46.
|
| 年度： | 2007 |
| 類別： |
期刊論文
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| 摘要： | |
| 關鍵字： | |
| 著作名稱： | Liu CW, Li X, Thompson D, Wooding K, Chang TL, Tang Z, Yu H, Thomas PJ, DeMartino GN. 2006 ATP binding and ATP hydrolysis play distinct roles in the function of 26S proteasome. Mol Cell. 24(1):39-50. |
| 年度： | 2006 |
| 類別： |
期刊論文
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| 摘要： | |
| 關鍵字： | |
| 著作名稱： | Chang TL, Kakhniashvili DG, Goodman SR. 2005. Spectrins E2/E3 ubiquitin-conjugating/ligating activity is diminished in sickle cells. Am. J. Hematol. 79(2):89-96. |
| 年度： | 2005 |
| 類別： |
期刊論文
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| 摘要： | |
| 關鍵字： | |
| 著作名稱： | Chang TL, Cubillos FF, Kakhniashvili DG, Goodman SR. Ankyrin is a target of spectrins E2/E3 ubiquitin-conjugating/ligating activity. Cell. Mol. Biol. 50(1):59-66. |
| 年度： | 2004 |
| 類別： |
期刊論文
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| 摘要： | |
| 關鍵字： | |
| 著作名稱： | Chang TL, Cubillos FF, Kakhniashvili DG, Goodman SR. Band 3 is a target protein of spectrins E2/E3 activity: implication for sickle cell disease and normal red blood cell aging. Cell. Mol. Biol. 50(2):171-177. |
| 年度： | 2004 |
| 類別： |
期刊論文
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| 摘要： | |
| 關鍵字： | |
| 著作名稱： | 林東毅, 李冠誼, 張家欽, 張翠玲 (2010) "電解液對於黃金電極吸附1,6-己烷基雙硫醇影響之研究" 臺南大學 環境與生態學報 第3卷第1期: 32-45 |
| 年度： | 2010 |
| 類別： |
期刊論文
臺南大學 環境與生態學報
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| 摘要： | |
| 關鍵字： | |
| 著作名稱： | Chang TL*, Wang CH (2012) Combination of quercetin and tannic acid as a potential proteasome inhibitor for cancer chemoprevention. Eleventh Annual AACR (American Association Cancer Research) International Conference on Frontiers in Cancer Prevention Research, Los Angeles, California. U.S.A. |
| 年度： | 2012 |
| 類別： |
會議論文
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| 摘要： | |
| 關鍵字： | |
| 著作名稱： | Chang TL*, Ou YD, Huang YH (2012) The Role of Ginsenosides in Inhibiting Ubiquitin-Activating Enzyme (E1). The 2012 Autumn Ginseng Conference, Seoul, Korea. (Oral presentation) |
| 年度： | 2012 |
| 類別： |
會議論文
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| 摘要： | |
| 關鍵字： | |
| 著作名稱： | Chang TL*, Ding HY, Kao YW(2008) Ginsenoside Rd is a Potential Inhibitor in Chymotypsin-like Activity of 26S Proteasome for Cancer Prevention. American Association Cancer Research Conference in Ubiquitin and Cancer.San Diego, California. U.S.A. |
| 年度： | 2008 |
| 類別： |
會議論文
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| 摘要： | |
| 關鍵字： | |
| 著作名稱： | Chang TL*, Lin MN, Chang CJ, Lee WY, Huang YW, Fan K. (2008) The roles of ubiquitin and 26S proteasome in human obesity. The Obesity Societys 2008 Annual Scientific Meeting. Phoenix, Arizona. U.S.A. |
| 年度： | 2008 |
| 類別： |
會議論文
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| 摘要： | |
| 關鍵字： | |
| 著作名稱： | Ding HY, Chang TL (2009) Antioxidant and Inhibition 26S Proteasome Activity from Rubus chingii 第24屆天然藥物研討會 高雄醫科大學 |
| 年度： | 2009 |
| 類別： |
會議論文
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| 摘要： | |
| 關鍵字： | |
| 著作名稱： | 林東毅, 李冠誼, 張家欽*,張翠玲 (2009)“26S Proteasome偵測系統中電解液對於黃金電極吸附HDT影響之研究”九十八年中國材料科學年會 國立東華大學 |
| 年度： | 2009 |
| 類別： |
會議論文
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| 摘要： | |
| 關鍵字： | |