國立臺南大學專任教師基本資料
姓名黃銘志
系所生物科技學系
校內分機7730
EMAILmingchih39@mail.nutn.edu.tw
辦公室06-260-6123轉7730
網址 
專長/研究領域系統生物學 水產工學
學位畢業學校國別主修學門修業期間
博士東京大學日本水圈生物工學2001/4-2004/3
服務機關部門系所職稱服務期間
宜蘭縣政府農業局漁業課技士1998/12-1999/3
台灣省政府農林廳漁業局養殖科技佐1999/4-1999/6
行政院農業委員會漁業署養殖沿近海漁業科技佐1999/7-2001/2
行政院農業委員會漁業署養殖沿近海漁業科技士2001/3-2004/6
日本理化學研究所基因總合研究中心準研究員2004/7~2008/3
日本理化學研究所基幹研究所準研究員2008/4~2008/8
國立台南大學生物科技學系助理教授2008/8-2014/2
國立台南大學生物科技學系副教授2014/3-
著作名稱:水から出た魚たち―ムツゴロウとトビハゼの挑戦 (ISBN:978-4905930174)
年度:2015
類別: 學術專書
摘要:
關鍵字:
著作名稱:2012文化語言教學國際學術研討會論文集-論烏龍派出所的文化美學(ISBN 978-986-03-5751-6)
年度:2012
類別: 學術專書
摘要:
關鍵字:烏龍派出所
著作名稱:海洋文化國際學術研討會會後論文集. 論清代曹廷杰《西伯利亞東偏紀要》中的麻勒特魚 2016.
年度:2016
類別: 學術專書
摘要:曹廷杰(1850-1926)生於清朝末期,當時俄國勢力在東北擴張,他曾到東北及黑龍江等地實地考察,並記載當地民族、習俗、生物種類等,為晚清的東北、黑龍江等地留下珍貴的文化史料。他在光緒十一年(1885)四月奉命到伯力(今俄羅斯哈巴羅夫斯克)一帶勘查邊疆情勢,歷盡艱辛,往返一萬六千餘里。 他的論著《西伯利亞東偏紀要》以親身見聞並徵引群書,闡明黑龍江北岸、烏蘇里江東岸等地的中國領土及風土民情。文本記錄黑斤濟勒彌人等當地住民的生活習慣。東北盛產魚類,由於多數記載魚類使用當地民族語言,以致民國以後的研究者進行考證時,有部分文獻難以解讀,容易混淆。《西伯利亞東偏紀要》提到「東北海口有大魚,長一二丈大一二圍,其頭有孔如江豚涉波,孔窩中噴水高一二丈,訇然有聲,可聞數里。黑斤濟勒彌人通呼為麻勒特魚。」曹氏指出:「麻特哈魚,即遼、金史《本記》所載之牛魚也。」麻勒特魚或麻特哈魚究竟是什麼魚? 本文解讀曹氏著作,配合生物種類調查,認為麻勒特魚與麻特哈魚應指相同生物種類,且類似鯨豚類等海洋哺乳動物。當代有部分文獻將麻勒特魚解釋為鰉魚(Huso dauricus),可能是受到清代之前的史書提及「牛魚」解釋的誤導。透過經庫頁島鯨豚類調查資料及盛行於東北之原始宗教薩滿教動物崇拜等民族學文獻比較,本文可推論出《西伯利亞東偏紀要》敘述的麻勒特魚,極有可能是現今的虎鯨(Orcinus orca)。
關鍵字:麻勒特魚
著作名稱:Teleosts, Evolutionary development, diversity and behavioral ecology
年度:2015
類別: 學術專書
摘要:The genes encoding skeletal muscle tropomyosin, with a unique coiled-coiled linear structure along the full-length molecule, were cloned from a wide range of fish species, and the deduced amino acid sequences, especially the amino acid substitutions between fish species from tropical to cold waters were discussed, based not only on the phylogenetic analyses but also on the species-specificity of thermodynamic properties as measured by calorimetric studies and thermal helical decaying profiles of the purified tropomyosins. The results obtained showed clear differences in thermal stability among the fish species examined, though the identity of amino acid sequences was higher than 92%. It suggests that only a few amino acid substitutions affect the overall stability of the tropomyosin molecules. Several residues especially located on the molecular surface were considered to be responsible for such stability difference. However, it was difficult to pinpoint the responsible residues. Thus it is likely that communications between the residues far from each other on the molecule exist. On the other hand, the molecular mass of these tropomyosins measured by mass spectrometry were generally higher than those calculated based on the deduced amino acid sequences, suggesting the presence of post-translational modifications which could also affect their thermal stability difference.
關鍵字:fish, tropomyosin, evolution.
著作名稱:第一屆東亞語文社會國際研討會:以日本、韓國、越南為出發點。日本食魚文化對台灣之影響以鮪魚及壽司為中心之探討
年度:2012
類別: 學術專書
摘要:
關鍵字:鮪魚、壽司
著作名稱:A new species of Bathynomus Milne-Edwards, 1879 (Isopoda: Cirolanidae) from the southern Gulf of Mexico with a redescription of Bathynomus jamesi Kou, Chen and Li, 2017 from off Pratas Island, Taiwan
年度:2022
類別: 期刊論文 Journal of Natural History
摘要:Bathynomus jamesi Kou, Chen and Li, 2017 from Zhengbin fishing port in Keelung, Taiwan was identified by the shape of the distolateral corner of the uropodal endopod, shape of the clypeus, and the nucleotide sequences of the COI and 16S rRNA genes. Only two species of Bathynomus have previously been recorded from Taiwan, B. doederleini Ortmann, 1894, and B. decemspinosus Shih, 1972. Bathynomus kensleyi, previously known from the South China Sea as well as the South-east of Swain Reefs, Coral Sea, was primarily differentiated by the elongate and upturned pleotelson spines, but that characters is now shown to also occur in mature Bathynomus jamesi. Two specimens from the Gulf of Mexico (obtained from the Enoshima Aquarium in Japan) were compared to species of Bathynomus from the western North Atlantic. Sequence data showed that one of two samples was not B. giganteus Milne-Edwards, 1879, as had been assumed, and it did not match any other species of Bathynomus. That specimen was collected off the Yucatan Peninsula and is morphologically distinct from both B. giganteus (in the relative length of the antennal flagellum and length: width ratio of the pleotelson) and B. maxeyorum Shipley, Brooks, and Bruce in Shipley et al., 2016 (the number of pleotelson spines is 7 and distolateral corner is produced on uropodal exopod) it is here described B. yucatanensis sp. nov. Bathynomus is currently a very minor fisheries resource in Taiwan and Japan, but this find demonstrates the continuing importance of the fishing industry to marine biological exploration.
關鍵字:Cirolanidae, South China Sea, Taiwan, Bathynomus kensleyi, Bathynomus yucatanensis sp. nov.
著作名稱:Thermostability of tropomyosins from the fast skeletal muscles of tropical fish species.
年度:2019
類別: 期刊論文 Fish physiology and biochemistry
摘要:In order to investigate the species-specific heat tolerance of tropical fishes, the thermodynamic properties of muscle tropomyosin, a member of myofibrillar proteins, were compared among milkfish, tilapia, grouper, and mudskipper. The purified tropomyosins were subjected to differential scanning calorimetry and circular dichroism spectrometry. To unveil the relationship between the stability and the amino acid sequences, the muscle tropomyosin genes of the four species were also cloned, and their deduced amino acid sequences were compared. Thermodynamic analysis revealed that the milkfish tropomyosin showed lower refolding ability after thermal denaturation, compared with those of the other species. The amino acid sequences of these tropomyosins were similar to each other, with the identity being in the range of 95–96%.
關鍵字:Tropical fish . Muscle tropomyosin . Thermostability.Amino acid sequence
著作名稱:Availability of Myoglobin as a Molecular Marker for Phylogenetic Relationships of Fish
年度:2018
類別: 期刊論文 Journal of Agricultural Science and Technology B
摘要:In order to elucidate the phylogenetic relationship of fish, DNA and deduced amino acid sequences of myoglobin (Mb) were used for the phylogenetic analyses based on different approaches, namely, maximum likelihood (ML), neighbor joining (NJ), unweighted pair group method with arithmetic means (UPGMA) and maximum parsimony (MP) methods in comparison with the conventional molecular markers, mitochondrial cytochrome b (cyt-b) and cytochrome c oxidase subunit I (COI). The phylogenetic trees drawn based on Mb sequences were similar to those by the traditional classification based on the other molecular markers. The primary and secondary structures, as well as the modeled tertiary structures of Mbs were similar to each other, but were clearly distinguishable among those species. Such differences in structure would be associated with adaptation of Mb molecule to the physiological conditions of each species. These results suggest that Mb can be a molecular marker for the phylogenetic relationship of fish.
關鍵字:Myoglobin, mitochondrial cytochrome b (cyt-b), cytochrome c oxidase subunit I (COI), phylogeny.
著作名稱:Evaluation of Biochemical Properties of Burnt and Normal Meat in Pacific Bluefin Tuna (Thunnus orientalis)
年度:2017
類別: 期刊論文 International Journal of Nutrition and Food Sciences
摘要:Abstract: Burnt meat is one of the unusual meats often occurring in tuna species. In order to reveal the biochemical properties of burnt meat in tuna, burnt meat and normal meat samples were collected to examine the quality parameter including pH, color parameters, lactic acid content, the activities of antioxidant enzymes, and the levels of thiobarbituric acid reactive substances (TBARS) and scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. The results showed pH was significantly lower in burnt meat than in normal meat, and the lactic acid content was generally higher in burnt meat. In color parameters, the L* and b* values were higher in burnt meat. Besides, the activities of antioxidant enzymes, namely, superoxide dismutase (SOD),glutathione reductase (GR) and catalase (CAT) in normal meat were higher than those in burnt meat. TBARS levels were significantly higher in burnt meat than in normal meat. It is thus likely that strong struggle of fish throughout catch and high water temperature induce partial inactivation of these antioxidant enzymes, resulting in free radical formation which triggers the occurrence of burnt meat. This fact could be beneficial for aquaculture and livestock industry of tunas to prevent burnt meat.
關鍵字:Burnt Meat, Bluefin Tuna, Antioxidant Activity, Lipid Oxidation
著作名稱:Sexual maturation of Girella punctata and G. leonina (Perciformes: Girellidae) in the neritic sea off the Pacific coast of Japan
年度:2017
類別: 期刊論文 Coastal marine science
摘要:The spawning ecology of the girellid fishes in Japanese waters has been reported almost only for Girella punctata, whereas little is known about Girella leonina. We examined sexual maturation of G. punctata and G. leonina collected from the coastal waters (neritic) of the Izu Peninsula and Tanegashima Island, based on gonadosomatic index (GSI) and gametogenesis, in order to clarify interspecific differences in spawning ecology. The GSI values of G. punctata in the Tanegashima Island region were markedly high in March, a month prior to the peak in April in the Izu Peninsula region. Girella punctata with elevated GSI had histologically mature ovaries and testes in spring in the both regions, suggesting spawning at this time of year. In contrast, the GSI values of G. leonina were much lower in the both regions, but the maximum GSI of both male and female G. leonina were slightly higher in the Tanegashima Island region than in the Izu Peninsula region. Furthermore, oogenesis in G. leonina was more advanced in the Tanegashima Island region. It is thus probable that Tanegashima Island is relatively near the spawning ground of G. leonina.
關鍵字:Girella punctata, Girella leonina
著作名稱:The complete mitochondrial genome of the gnomefish Scombrops boops (Teleostei, Perciformes, Scombropidae) from the Pacific Ocean off the Japanese Islands. (SCI, IP:1.701, R128/165, Genetic & Heredity)
年度:2014
類別: 期刊論文 Mitochondrial DNA
摘要:The complete mitochondrial genome of the gnomefish Scombrops boops was determined by a PCR-based method. The total length of mitochondrial DNA (mtDNA) was 16,517 bp, including 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes and one control region. The mitochondrial gene arrangement of the gnomefish mtDNA was identical to those of typical teleosts. This is the first report of the complete mitochondrial genome of a member of the Scombropidae family and will be useful for the development of molecular tools for ecological research.
關鍵字:Fish, gnomefish, mtDNA, Scombropid fish
著作名稱:Gene network analysis to determine the effects ofantioxidant treatment in a rat model of neonatal hypoxic-ischemic encephalopathy
年度:2011
類別: 期刊論文 J Mol Neurosci
摘要:Neonatal hypoxic-ischemic (HI) encephalopathy can lead to severe brain damage, and is a common cause of neurological handicaps in adulthood. HI can be resolved by the administration of an antioxidant such as 3-methyl-1-phenyl-2-pyrazolin-5-one (MCI-186). In the present study, we performed comprehensive gene expression and gene network analyses using a DNA microarray to elucidate the molecular events responsible for the selective vulnerability of neurons in neonatal HI brain insult and to examine the underlying mechanisms of the effect of MCI-186 on the pathophysiological events in this condition. We used the modified Levine method (Rice model), which has been widely used as an animal model of this condition. A large difference in gene expression was observed between the Rice model and the control group. Up- and downregulated genes after the HI brain insult were mainly related to immune responses and cell death, and neuronal activity, respectively. The effect of MCI-186 administration on gene expression was much less than and contrary to that of the HI brain insult, reflecting the protective effect of MCI-186 in HI brain insult.
關鍵字:
著作名稱:Characterization of two tropomyosin isoforms from the fast skeletal muscle of bluefin tuna Thunnus thynnus orientalis
年度:2010
類別: 期刊論文 Archives of Biochemistry and Biophysics
摘要:Fast skeletal muscle tropomyosin (TM) of tunas is composed of nearly equimolar amount of two isoforms designated α-TM and β-TM expediently based on their migration behavior in SDS–PAGE, whereas corresponding TMs from the other fish species are homogenous (α-type). The presence of β-TM is thus specific to tunas so far. The amino acid sequence of β-TM from bluefin tuna Thunnus thynnus orientalis, which has not been revealed to date unlike α-TM, was successfully obtained in this study by cDNA cloning. The coding region of β-TM cDNA comprised of an open reading frame of 855 bp encoding 284 amino acid residues, like most of the TMs. Unexpectedly, the sequence of β-TM showed high similarity to those of other vertebrate α-type TMs including tuna α-TM. Phylogenetic analysis also showed that β-TM has the closest relationship with α-TM of tuna. This fact was quite unlike the relation of mammalian α- and β-TMs. Based on the distribution of amino acid substitutions, it was suggested that tuna TM isoforms are the products of different genes. By thermodynamic analysis of native and reconstituted TMs, it was demonstrated that β-TM is less thermostable than α-TM. Proteolytic digestion also supported the lower stability of the former.
關鍵字:Tropomyosin,Tuna Isoform, Primary structure, Skeletal muscle, Thermostability
著作名稱:XiuYu Shi, Ming-Chih Huang, Atsushi Ishii, Shuichi Yoshida, Motohiro Okada, Kohtaro Morita, Hiroshi Nagafuji, Sawa Yasumoto, Sunao Kaneko, Toshio Kojima & Shinichi Hirose. Mutational analysis of GABRG2 in a Japanese cohort with childhood epilepsies. (SCI IF3.755)
年度:2010
類別: 期刊論文 Journal of Human Genetics
摘要:A few mutations in the gene encoding the gamma 2 subunit of the gamma-aminobutyric acid receptor type A (GABRG2) have been reported in various types of epilepsy. The aim of this study is to investigate the role of GABRG2 in the pathogenesis of childhood epilepsy in a large Japanese cohort. Genetic analysis of GABRG2 was performed on 140 Japanese patients with various childhood epilepsies largely including Dravet syndrome and genetic epilepsy with febrile seizures plus. The mutational analysis identified one novel missense mutation of GABRG2 (c.236A>G: p.N40S) in a patient with generalized tonic-clonic seizures (GTCS). The mutation was heterozygous and replacing a highly conserved Asn residue with a Ser. The affected amino acid was located at residue 40 of the mature GABRG2 protein, which was near the first one of two high-affinity benzodiazepine-binding domains of the γ2 subunit (Lys-41-Trp-82). This mutation in such an important position may hamper the function of the channel and contribute to the case’s pathogenesis of GTCS.
關鍵字:gamma-aminobutyric acid receptor type A
著作名稱:Ming-Chih Huang, Motohiro Okada, Fubito Nakatsu, Hirokazu Oguni, Masatoshi Ito, Kohtaro Morita, Hiroshi Nagafuji, Shinichi Hirose, Sunao Kaneko, Hiroshi Ohno, Yoshiyuki Sakaki and Toshio Kojima. (2007) Mutation Screening of AP3M2 in Japanese Epilepsy Patients. Brain Dev. 29: 462-467. (SCI)
年度:2007
類別: 期刊論文 Brain and Development
摘要:Evidence that some types of epilepsies show strong genetic predisposition has been well documented. AP3M2 is considered to be an epileptogenic gene because AP3M2 knockout mice exhibit symptoms of spontaneous epileptic seizures. In order to investigate whether the AP3M2 gene causes susceptibility to epilepsy, we performed mutation screening of the genomic DNA of 190 patients with six epilepsy types; this screening involved all the 9 exons and the relevant exon–intron boundaries of AP3M2. Although neither missense nor nonsense mutations were detected, we identified 21 sequence variations, of which 16 variations were novel. Of the 21 variations, 11 were detected in 5′ and 3′ UTRs, while the remaining variations were detected in introns. Although the present study failed to identify the possible AP3M2 mutations that may cause epilepsy, our results suggest that some AP3M2 mutations still remain candidates for unmapped disorders including epilepsy, febrile seizure, and other neuronal developmental disorders associated with functional abnormalities of GABAergic transmission.
關鍵字: AP3M2, Epilepsy
著作名稱:Ming-Chih Huang, Yoshihira Ochiai.(2005)Fish fast skeletal muscle tropomyosins show species-specific thermal stability. Comp Biochem Physiol B Biochem Mol Biol. 141:461-471. (SCI)
年度:2005
類別: 期刊論文 Comp Biochem Physiol B Biochem Mol Biol.
摘要:Tropomyosin (TM) was isolated from the fast skeletal muscle of six fish species, whose amino acid sequences of this protein have already been revealed. The thermal stability of these TMs was measured by differential scanning calorimetry (DSC) and circular dichroism (CD), while the molecular weights were measured by mass spectrometry. The results showed clear differences in thermostability among these fish TMs, though the identity of amino acid sequences was more than 93.3%. Therefore, only a few amino acid substitutions could affect the overall stability of the TM molecule. Especially, several residues located on the molecular surface were considered to be responsible for such stability difference. In contrast, the molecular weights of these TMs as measured by mass spectrometry were higher than those calculated from amino acid composition, suggesting the presence of post-translational modification(s) which could also affect their thermal stability.
關鍵字:tropomyosins
著作名稱:Ming-Chih Huang, Yoshihira Ochiai, and Shugo Watabe. (2004)Structural and thermodynamic characterization of tropomyosin from fast skeletal muscle of bluefin tuna. Fish. Sci. 70:667-674. (SCI)
年度:2004
類別: 期刊論文 Fish. Sci.
摘要:Tuna tropomyosin is a mixture of nearly equimolar amounts of two isoforms (designated a and b). cDNA encoding the a form was cloned from bluefin tuna Thunnus thynnus fast skeletal muscle. The full-length cDNA contained 1220 bp, comprising an open reading frame of 855 bp encoding 284 amino acid residues, flanked by 5¢-untranslational regions (156 bp) and 3¢-untranslational regions (209 bp). The deduced amino acid sequence showed considerably high homology in a range of 93.7–98.6% to those of other vertebrate a-type tropomyosins. In phylogenetic analysis, bluefin tuna tropomyosin showed the closest relationship with the white croaker counterpart. The predicted mass was 32 919 Da, and isoelectric point was 4.50, assuming acetylation of the Nterminus. By differential scanning calorimetry, bluefin tuna tropomyosin gave two major endothermic peaks at 29.3 and 41.5∞C, probably caused by the presence of two isoforms. Circular dichroism spectra supported such a unique denaturation profile.
關鍵字:bluefin tuna
著作名稱: cDNA cloning and thermodynamic properties of tropomyosin from walleye pollack Theragra chalcogramma fast skeletal muscle.
年度:2003
類別: 期刊論文 Fish. Sci.
摘要:cDNA encoding tropomyosin was cloned from walleye pollack Theragra chalcogramma fast skeletal muscle. The predicted mass was 32 588 Da and isoelectric point, 4.55, assuming acetylation of the N-terminus. The full-length cDNA contained 1168 bp, comprising a 5¢untranslational region (97 bp), a 3¢-untranslational region (219 bp) and an open reading frame of 855 bp encoding 284 amino acid residues. The deduced amino acid sequence showed considerably high homology in a range of 94–97% to those of other vertebrate a-type tropomyosins. In phylogenetic analysis, walleye pollack tropomyosin showed the closest relationship with the Atlantic salmon counterpart. By differential scanning calorimetry, pollack tropomyosin gave only one major endothermic peak at around 44∞C. Circular dichroism spectra supported this denaturation profile of pollack tropomyosin.
關鍵字:circular dichroism, cDNA cloning, differential scanning calorimetry, fast skeletal muscle, primary structure, tropomyosin, walleye pollack.
著作名稱:Chen-Huei Huang, Ming-Chih Huang and Ping-Chun Hou. (1998) Effect of dietary lipids on fatty acid composition and lipid peroxidation in sarcoplasmic reticulum of hybrid tilapia, Oreochromis niloticus × O. aureus. Comp. Biochem. Physiol. 120B:331-336.
年度:1998
類別: 期刊論文 Comp. Biochem. Physiol.
摘要:The effects of dietary lipid sources on the growth response of hybrid tilapia (Oreochromis niloticus×O. aureus) were investigated. Enzymic lipid peroxidation in the sarcoplasmic reticulum (SR) of these fish fed different dietary lipids was also studied. Experimental fish were given diets containing no additional lipids (control), lard, soybean oil, menhaden fish oil and methyl esters of n-3 highly unsaturated fatty acids (HUFA) for 10 weeks. There was no difference in survival rates among test groups. Compared to fish supplied with dietary lipids, fish consumed control diet with trace lipids (0.02%) were low in final body weights and muscle lipids. The specific growth rates and weight gains of fish fed soybean oil and fish oil were significantly higher than other groups (P0.05). Fatty acid compositions of muscle and SR reflected the fatty acid compositions of dietary lipids. The thiobarbituric acid reactive substances produced by iron catalyzed NADH-dependent lipid peroxidation were significantly different (P0.05) among SR isolated from fish fed different dietary lipids. Lipid peroxidation activity of HUFA group was the highest, followed by fish oil, soybean oil, lard and control group.
關鍵字:Dietary lipids, Docosahexaenoic acid, Fatty acid composition, Fish oil, Hybrid tilapia, Lard, Lipid peroxidation, Sarcoplasmic reticulum, Soybean oil
著作名稱:Exploring α tropomyosin as a molecular marker for studying higher-level phylogenetics in commercial fish
年度:2022
類別: 期刊論文 Modern Environmental Science and Engineering
摘要:Tropomyosin is a structural protein located in muscle cells. It has the same conservative characteristics as recombination activating gene 1 (RAG-1). RAG-1 is commonly used in higher-level phylogenetic studies of birds. Therefore, this study compared five mitochondrial DNAs (12S rRNA, 16S rRNA, cytochrome b, cytochrome c oxidase subunit I, and NADH-ubiquinone oxidoreductase chain 5) commonly used in fish phylogeny with nucleotides and amino acids of α tropomyosin. We are exploring the feasibility of α tropomyosin as a molecular marker for higher-level phylogenetic studies of fish. In this study, 32 fish five mitochondrial DNA genesand α tropomyosin nucleotide and amino acid sequences were used to draw a phylogenetic tree using the Maximum Likelihood method in the Molecular Evolutionary Genetics Analysis 11 (MEGA 11) software. The results show that the phylogenetic tree drawn with α tropomyosin amino acids as molecular markers has the closest results in fish higher-level phylogenetics with the fish phylogenetic tree established by Betancur-R in 2017. It can be seen that α tropomyosin amino acid is feasible as a molecular marker for studying higher-level phylogenetics of fish.
關鍵字:tropomyosin
著作名稱:思氏不對稱鰓虱(Discorsobopyrus stebbingi(Nierstrasz & Brender à Brandis, 1923))(甲殼綱:等足目:鰓虱科)寄主新記錄及形態描述與18SrRNA分析 A New Host for Discorsobopyrus stebbingi (Nierstrasz & Brender à Brandis, 1923), with Morphological Remarks and an Analysis of Its 18S rRNA (Crustacea: Isopoda: Bopyridae)
年度:2020
類別: 期刊論文 國立臺灣博物館學刊
摘要:鰓虱科(Bopyridae)生物屬於等足目(Isopoda)上蝦亞目(Epicaridea),主要寄生於十足目(Decapoda)生物如蝦蟹之鰓腔,吸食宿主血淋巴液維生。被寄生之蝦蟹會造成生長不良、生殖障礙等問題造成族群量之減少。人類誤食可能導致過敏等食安風險,所以鰓虱之種類鑑定、生態及生活週期有必要被研究了解。不對稱鰓虱屬Discorsobopyrus是2004年自真蝦鰓虱亞科Bopyrinae分出之新屬,目前該屬只有一種生物記錄為思氏不對稱鰓虱(Discorsobopyrus stebbingi (Nierstrasz & Brender à Brandis, 1923)),該物種在文獻中記載宿主為東方異腕蝦(Heterocarpus sibogae de Man, 1917),本次發現為寄主新紀錄,其特徵有研究價值。2018年9月12日在屏東東港林氏異腕蝦(H. hayashii Crosnier, 1988)採樣中發現疑似上述寄生蟲。經解剖顯微鏡、電子顯微鏡觀察後發現:雌蟲與雄蟲間的體型差異極大、雌蟲體呈卵圓形 頭部較長,呈倒三角形並向後逐漸縮窄 顎足周圍具有小短刺,沒有觸鬚,兩面皆呈裂片狀,顎足上側具有些許小突起的構造以及稀疏的剛毛,第一對卵板呈不規則卵形或三角形,內面平滑。第一觸角(antenna-1)和第二觸角(antenna-2)各存在一節等,且其宿主同樣屬於長額蝦科(Pandalidae)。根據上述特徵鑑定為思氏不對稱鰓虱(D. stebbingi)。以18S rRNA序列作分子系統學分析結果得知,思氏不對稱鰓虱和寄生在長額蝦科(Pandalus borealis)之Hemiarthrus abdominalis親緣最為相近。本研究期望能提供不對稱鰓虱屬記錄,並作為生物多樣性及海洋資源管理之參考。
關鍵字:思氏不對稱鰓虱
著作名稱:Observations on Metanephrops neptunus (Bruce, 1965) (Crustacea: Astacidea: Nephropidae) from the Pratas Islands, South China Sea
年度:2020
類別: 期刊論文 Crustacean Research
摘要:Abstract.̶ The Neptune lobster, Metanephrops neptunus (Bruce, 1965), is reported from 300–600 m depths off the Pratas Islands southwest of Taiwan in the South China Sea. Seven males and three ovigerous females were captured in July 2019. Key morphological features and internal structures are described, with particular attention to the chelae, mandibles, gills, cephalothoracic and abdominal sculpturing, and gastric mills. The red-and-white coloration is described and illustrated from fresh specimens. The geographic range, depth of occurrence, stomach contents, and fecundity of the species are also noted.
關鍵字:distribution, gastric mill, gill, morphology, red-headed lobster, stomach contents
著作名稱:First record of Holophryxus fusiformis Shiino, 1937 (Crustacea, Isopoda, Dajidae) from the sakura shrimp, Lucensosergia lucens, in Taiwan
年度:2018
類別: 期刊論文 Crustacean Research47: .
摘要:Holophryxus fusiformis Shiino, 1937, a species of dajid isopod that attaches to the carapace of sakura shrimp, Lucensosergia lucens (Hansen, 1922), is reported from the sea of Taiwan for the first time. This species was first described as infesting Prehensilosergia prehensilis (Bate, 1881) from Kanbara, Shizuoka Prefecture, Japan. The current finding represents the second occurrence of H. fusiformis and identifies a new host. Approximately 1% of the sakura shrimps in Yilan, Taiwan are infected by H. fusiformis. Holophryxus fusiformis has been found offshore from both Yilan, Taiwan and Nagasaki and in Suruga Bay, Japan; thus, the distribution of the parasite possibly follows the Kuroshio Current. When large quantities of parasites occur in the sea of Taiwan, the parasites are likely to flow into the Japanese waters along with the Kuroshio Current, subsequently influencing the production of the Japanese sakura shrimps. The establishment of a monitoring program for infection of the dajid in sakura shrimps between Taiwan and Japan is recommended. This study provides additional information on this species, including a new host, distribution, redescription, infection rate, and morphological variation.
關鍵字:North West Pacific, Kuroshio current, Holophryxus, crustacean parasite
著作名稱:宜蘭產正櫻蝦梭形附頭虱(Holophryxus fusiformis)的發現與鑑定
年度:2017
類別: 期刊論文 台灣水產
摘要:正櫻蝦梭形附頭虱 (Holophryxus fusiformis, Shino, 1937) 是等足目 (Isopoda) 檐虱科(Daidae) 附頭風屬(Holophryxus)的一種,是蝦類寄生蟲。主要以正櫻蝦(俗名:櫻花蝦, Lucensosergia lucens)為宿主, 感染位置以蝦類頭胸甲正上方, 蟲體以尾前頭後方式附著。特徵如下:體長5-8mm (平均6.2mm, n8),體寬1.8-3.8mm (平均2.9mm, n8),身體成梭形囊狀,顏色由米色、淺黃至橘色均有發現。無明顯體節, 附屬肢退化,前軀幹具有四對鋸狀摺緣構造,易誤認為步足。特徵與所屬等足目(Isopoda)有很大不同。雄性呈矮雄狀,常附著在雌性育兒袋中。臺灣宜蘭產正櫻蝦約有1% 感染率,被感染之正櫻蝦可能導致不孕化。宜蘭產正櫻蝦梭形附頭經鑑定與日本駿河灣發現正櫻蝦梭形附頭相同種類。
關鍵字:正櫻蝦梭形附頭虱 ; 櫻花蝦 ; 寄生蟲
著作名稱:Yu-Cheng You and Ming-Chih Huang. 2016. The tropomyosin structure and phylogenetic analysis of five fish species. International Journal of Science and Engineering. 6(1):23-42
年度:2016
類別: 期刊論文 International Journal of Science and Engineering
摘要: Fish are vertebrates and the largest number of biological species, they also have high degree of biodiversity. In order to survival in different environments, fish develop many patterns or mechanisms to adapt surrounding changes. For this reason, there are many fishes look similar but not the same species. To identify a correct species is an difficult but important issue for fish science. Today species identification and classification of commonly used molecular markers including mitochondrial cytochrome b and cytochrome c oxidase subunit I. But not all of them are applicable for above systems. This study wanted to use tropomyosin as a new molecular mark ers for fish identification, classification and development. Movement proteins are long-standing presence protein, one of movement proteins, tropomyosin can coordination of regulation and the promotion of muscle contraction with actin and myosin. This study use highly conserved features of tropomyosins as a molecul ar markers by DNA or protein sequences. The use of the logged database of fish muscle tropomyosin amino acid and DNA sequences to calculate and simulation five fish relationship including white croaker (Pennahia argentata ), northern bluefin hula (Thunnus thynnu s), tiger pufferfish (Tnktfugu rnbr ipes), zebrafish (Donio rerio), Atlantic salmon (Salmo salar). We will investigate whether tropomyosin can become a good molecular markers or not. By results, we found the primary and second structure of tropomyosin were different among the five fishes. The various of structure could help the fish with some different biological functions to adapt environment. The results of used tropomyosin as molecular marker to establish phylogenetic tree were more close to traditional theory than used other marker s. This means that tropomyosin can be used as molecular markers for fish evolution and phylogenetic analysis . The study wish to take views to explain the relationship between biological evolution by tropomyosin and a new molecular marker to species identification.
關鍵字:fish、muscle、tropomyosin、cytochrome b、cytochrome c oxidase subunit I
著作名稱:Structural and Thermodynamic Properties of Tropomyosins from the Ordinary and Hybrid Tilapia
年度:2016
類別: 期刊論文 Fish & Ocean Opj.
摘要:Tilapia is a very common aquaculture species in Taiwan and both the so-called freshwater tilapia (Oreochromis niloticus) and seawater tilapia (O. mossambica) are available. In order to characterize muscle tropomyosin from these tilapia and hybrid ones (O. mossambica × O. niloticus ), the biochemical properties of tropomyosin among these tilapia species were analysed. The nucleotide sequences of tropomyosin genes were determined by cDNA cloning, and the amino acid sequences were deduced. Fast skeletal muscle tropomyosins were isolated by the combination of isoelectric point precipitation and salting out with ammonium sulfate. The thermodynamic properties of the purified tropomyosins were examined by differential scanning calorimetry (DSC) and circular dichroism (CD) spectrometry. The results showed that those tropomyosins were identical to each other including the deduced amino acid sequence, thermodynamic patterns as measured by DSC and CD, except the partial differences in the nucleotide sequence, namely, at 99th (A/G), 183rd (T/C) and 846th (T/C) positions in the open reading frame.
關鍵字:Tilapia; Hybrid; Tropomyosin; cDNA cloning; Fast skeletal muscle; Thermal stability
著作名稱:The biochemical property between “Sik-Bah” and burnt meat from Pacific Bluefin tuna (Thunnus oriental)
年度:2015
類別: 期刊論文 International Journal of Science and Engineering
摘要: From April to July, the Pacific bluefin tuna (Thunnus thynnus orientalis) will migrate to Taiwans east coast every year. The bluefin tuna is one of the important fishery species in Taiwan. Since bluefin tuna taste good, it often as sashimi and other Japanese cuisine advanced ingredients. In those years, the reduced amount of resources to make bluefin tuna renew high prices records in the international market. It is important for meat colors of bluefin tunas, red meat is fresh and quality assurance. But the Donggang fishermen legend meat that presents a pale, soft and a little sour of tuna meat known as "cooked-meat" (pronounced Taiwanese to Sik-Bah). Cooked-meat is an abnormal meat, often occurs in the case of tuna muscle, especially bluefin tuna (Thunnus thynnus), bigeye tuna (Thunnus obesus) and yellowfin tuna (Thunnus albacares). Donggang fishermen describe the main features of cooked-meat as follow; pale flesh, lack of flexibility and sour. Foreign reports "burnt meat" are very similar to cooked-meat because their features also have pale, lack of flexibility, slightly sour and others. Is the "cooked-meat" same as "burnt meat"? Because Taiwan have no any references, it is difficult to determine. This paper aims to explore the biochemical property between "burnt meat" and "cooked-meat". In order to know the characteristics of cooked-meat, interviews for tuna related worker 30, and sampling cooked meat for laboratory analysis including pH, color quality (L*, a*, b*) and electrophoresis. The results of interview by Donggang fishermen show that the property of cooked-meat including pale (90%), soft (73%), sour (67%), rancid (53%). May cause cooked meat at a temperature factor was the highest (93%), followed by storage mode (53%), the period time of death (37%), sun or moon irradiation (30%), gender impact (30%), species strain (27%), death struggle (20%). The results of the data showed that the pH value of cooked-meat (5.56 ± 0.1, n14) was lower than normal meat (5.96 ± 0.2, n14). The color values of brightness (L*) (39.61 ± 3.36 vs 29.07 ± 2.19, n14), blue and yellow degree (b*) (12.41 ± 2.88 vs 7.77 ± 2.90, n14) in cooked-meat significantly higher than normal one. The SDS-PAGE electrophoresis analysis for cooked-meat from Donggang collected shown near 50 kDa protein band degradation. To be compared with papers reported from Yamashita and Ochiai, the degraded protein was creatine kinase. From the above results, the conclusion that "cooked-meat" was the same as "burnt meat." In this study, we make sure that "cooked-meat" was "burnt meat." We will design new study by burnt meat references.
關鍵字:黑鮪、熟肉
著作名稱:Mudskippers in Tainan: The ecology and lifestyleof mudskipper Periophthalmus modestus in Tainan coastal wetland.
年度:2013
類別: 期刊論文 International Journal of Science and Engineering
摘要:The mudskipper is one of the important wetland species. It plays a key role in the conservation of wetlands and waterfowl. The special and changing environment has given rise to the evolution of many physiological mechanisms adapted to wetlands. With high sensitivity to environmental changes, mudskippers are considered as indicator organisms of wetland environmental changes. This survey is focused on the ecology and lifestyle of the mudskippers in Tainan wetland, including mudskipper species, population size, Body Mass Index change, environmental change, ecological behavior, courtship and reproduction, nest analysis, etc. The results show that there are at least two species; namely, Periophthalmus modestus and Boleophthalmus pectinirostris in the coastal wetlands of Tainan with the population size growing and declining dynamically along with the natural environment and human activity. Moreover, there are growth changes affected by the environment. Based on three observation points; namely, Keliao, Mangrove and Anping, mudskippers grow best in Mangrove while thrive poorly in Anping. Salinity is the main environmental factor while dissolved oxygen is the second. The nest is found to have a dendritic structure. The mudskippers environmental tolerance is not strong as expected. This may cause mudskippers to disappear due to their vulnerability to environmental changes such as embankments, fish farms, roads and other public buildings that bring cement and affect their habitat. Relevant authorities are expected to include mudskippers in the list of endangered species and begin conservation measures that give importance to mudskipper ecology and related wetland research that will help develop a sustainable society and country.
關鍵字:Mudskipper
著作名稱:鮪類異常肉發生之原因
年度:2011
類別: 期刊論文 Taiwan Fisheries Association
摘要:遠洋鰹鮪漁業是台灣重要的產業,然而鮪魚肉易出現異常現象。本篇介紹鮪類易出現隻異常肉現象及可能產生原因。
關鍵字:鮪魚 ; 肉質 ; 異常肉
著作名稱:Thermodynamic characterization of skeletal muscle tropomyosin from mudskipper (Boleophthalmus pectinirostris)(大彈塗魚肌肉原肌凝蛋白脂熱安定性探討)
年度:2011
類別: 期刊論文 Journal of Ecology and Environmental Sciences
摘要:Mudskipper (Periophthalmini boleophthalmus) is widely distributed in coastal areas and wetland in Taiwan. The fish has a well adaptability for changes to the aquatic environment like salinity, dissolved oxygen and temperature. The purpose of this study is to investigate the biochemical characteristics and thermal stability of muscle tropomyosin (TM) from mudskipper. We extracted the crude TM from mudskipper skeletal muscle by high speed centrifugation, isoelectric point, and salting out TM with ammonium sulfate. Then we purified TM by column chromatography and detected its characteristics by electrophoresis gel analysis, circular dichroism (CD), and differential scanning calorimetry (DSC). The results show the muscle TM from mudskipper skeletal muscle has only one major form of TM. The DSC analysis showed Tm near 44 ℃. The CD spectra know the major structure of TM protein is α-helix. We wish this study can provide some data for biodiversity and evolution in mudskipper, and can be useful for protein structure study. 大彈塗魚(Periophthalmini boleophthalmus)是一種台灣西海岸濕地常見的濕地魚類。能適應半水半陸的潮間帶環境。這種魚類棲息於河口水域、沼澤及泥灘,在泥下建造垂直地道構成的洞內居住。由於大彈塗魚的生態特殊,濕地環境之溶氧、溫度、鹽度變化劇烈,大彈塗魚如何適應溼地環境乃至於如何運動彈跳,肌肉結構及組成是重要關鍵。 本次研究以肌肉纖維中保守性較高之肌肉纖維-原肌凝蛋白(TM)為研究對象,探討大彈塗魚肌肉纖維TM的熱安定性為主,並與其他魚種做分析比較。研究方法如下:利用超高速離心、等電點沉澱、硫銨分化及離子交換樹脂等方式找出精製大彈塗魚TM蛋白質的條件,經由一次電泳確認蛋白質純化後再用二次電泳分析有無isoform存在,經示差掃描熱量分析儀分析(DSC)及圓二光偏極光譜儀分析(CD)與其他魚種做比較。 研究與分析結果顯示,大彈塗魚肌肉纖維中TM以一種型態出現,沒有isoform,蛋白質結構以α-helix為主,熔點Tm為44℃。熱穩定性較黑鮪(Thunnus thynnus)及阿拉斯加鱈魚(Theragra chalcogramma)安定的現象。希望這些研究能夠更幫助了解濕地生物在濕地環境及生理適應之模式,了解生物多樣性及在演化上之意義。
關鍵字: mudskipper, tropomyosin (TM), circular dichroism (CD), differential scanning calorimetry (DSC)
著作名稱:High-resolution analysis of aberrant regions in autosomal chromosomes in human leukemia THP-1 cell line
年度:2009
類別: 期刊論文 BMC Research Notes
摘要:Background: THP-1 is a human monocytic leukemia cell line derived from a patient with acute monocytic leukemia. The cell line differentiates into macrophage-like cells by stimulation with phorbol myristate acetate (PMA). Although it has been used frequently as a model for macrophage differentiation in research including the FANTOM4/Genome Network Project, there are few reports on its genomic constitution. Therefore, we attempted to reveal the genomic aberrations in these cells with the microarray-based comparative genomic hybridization (aCGH) technique. Findings: We report large aberrations, including deletions 6p, 12p, 17p, and trisomy 8, and revealed breakpoints in the MLL and MLLT3 genes. Moreover, we found novel genomic aberrations such as a hemizygous narrow deletion partially containing the TP73 gene and homozygous deletions, including the CDKN2A, CDKN2B and PTEN genes. Conclusion: In this study, we identified 119 aberrant regions in autosomal chromosomes, and at least 16 of these aberrations were less than 100 kb, most of which were undetectable in the previous works. We also revealed a total of 4.6 Mb of homozygous deleted regions. Our results will provide a base to precisely understand studies involving the THP-1 cell line, especially the huge amount of data generated from the FANTOM4/Genome Network Project.
關鍵字:human leukemia THP-1 cell line
著作名稱:Chen-Huei Huang, Ming-Chih Huang and An-Chin Lee.(1998)Characteristics of lipid peroxidation in sarcoplasmic reticulum of Tilapia. Food Sci. 25:104-108.
年度:1998
類別: 期刊論文 Food Sci. (2001更名Taiwanese Journal of Agricultural Chemistry and Food Science)
摘要:To establish a model system for future lipid peroxidative and anti-oxidative research, some characteristics of an iron-catalyzed enzymic lipid peroxidation system in tilapia sarcoplasmic reticulum were investigated. Results of the experiments showed that this system preferred NADH to NADPH as the reducing agent. The rate of lipid peroxidation for the NADH added sample was significantly higher than for the NADPH added sample. The optimal pH for the reaction was 6.8. When the reaction temperature was raised from 1℃ to 25℃, the lipid peroxidation rates also increased. Since tilapia is a major cultured fish species in Taiwan and the supply is stable, the enzymic lipid peroxidation system in tilapia sarcoplasmic reticulum will be an excellent model for lipid peroxidative studies. 本研究以吳郭魚的肌漿網膜為材料,探討此生物膜中以鐵離子催化的酵素性脂質過氧化系統的特性,以作為研究脂質過氧化與抗氧化試驗的模型。研究結果顯示,此系統以NADH為還原劑時的脂質過氧化速率,顯著的高於使用NADPH為還原劑(P0.05),而這個系統的最佳反應酸鹼值為6.8。當反應溫度由1℃升到25℃時,反應速率也加快。由於吳郭魚是本省主要養殖魚種之一,因價廉,且無季節性限制,易於取得。所以吳郭魚肌漿網膜酵素性脂質過氧化系統,極適合作為本地研究脂質過氧化與抗氧化試驗的模型。
關鍵字:
著作名稱: Feasibility of α tropomyosin as a molecular marker for studying higher-level phylogenetics of fish.
年度:2022
類別: 會議論文
摘要:Tropomyosin is a structural protein located in muscle cells. It has the same conservative characteristics as recombination activating gene 1 (RAG-1). RAG-1 is commonly used in higher-level phylogenetic studies of birds. Therefore, this study compared five mitochondrial DNAs (12S rRNA, 16S rRNA, cytochrome b, cytochrome c oxidase subunit I, and NADH-ubiquinone oxidoreductase chain 5) commonly used in fish phylogeny with nucleotides and amino acids of α tropomyosin. We are exploring the feasibility of α tropomyosin as a molecular marker for higher-level phylogenetic studies of fish. In this study, 32 fish five mitochondrial DNA genesand α tropomyosin nucleotide and amino acid sequences were used to draw a phylogenetic tree using the Maximum Likelihood method in the Molecular Evolutionary Genetics Analysis 11 (MEGA 11) software. The results show that the phylogenetic tree drawn with α tropomyosin amino acids as molecular markers has the closest results in fish higher-level phylogenetics with the fish phylogenetic tree established by Betancur-R in 2017. It can be seen that α tropomyosin amino acid is feasible as a molecular marker for studying higher-level phylogenetics of fish.
關鍵字:tropomyosin
著作名稱:利用基因晶片技術促進水產育種及檢疫技術
年度:2021
類別: 會議論文
摘要:
關鍵字:基因晶片
著作名稱:2021日本水產學會春季)Broodstock analysis of an abnormal tuna caught in Taiwan (台湾で漁獲された異常マグロの親魚解析
年度:2021
類別: 會議論文
摘要:【Introduction】 On December 14, 2020, one individual of female tuna has been caught in the western waters of Xiaoliuqiu, Taiwan, with the size of bluefin tuna, anal fins of bigeye tuna and pectoral fins of yellowfin tuna. Besides, the fish had mature ovaries and developing eggs. The fishermen thought it was a hybrid with very low frequency. In order to find out whether this peculiar tuna is a hybrid or not, we tried to analyze the parent species of this fish with molecular biological methods. 【Materials and methods】 Using the principle of maternal inheritance, we tried to analyze the cyt b sequence of mitochondrial DNA, while microsatellite repeat sequence analysis was applied to paternal inheritance. The genomic DNA was isolated from the ordinary muscle, and primers were designed bases on the sequence of conserved regions. The PCR amplified products were sequenced. 【Results and discussion】 According to the cyt b sequence from the maternal genetic analysis, the mother of this peculiar tuna was demonstrated to be Pacific bluefin tuna (Thunnus orientalis). The patrilineal examination is still under analysis. Further research could contribute to breeding of tunas with useful genetic characters.
關鍵字:abnormal tuna
著作名稱:Molecular biological analysis of giant deep-sea isopod Bathynomus species caught in Pratas Island waters, Taiwan(台湾東沙島海域で漁獲されたグソクムシ類の分子生物学的種判別)
年度:2021
類別: 會議論文
摘要: Bathynomus species are deep-sea crustaceans distributed in the deep ocean floor of the Pacific, Atlantic and Indian Oceans. According to the references, there are at least three species of Bathynomus in the South China Sea, including B. doederleini, B. kensleyi and B. jamesi. The differences between the species of genus Bathynomus are very small and not easily distinguished based on the morphology (Fig. 1, Table 1). The upwardly curved pleotelsonic spines are often considered to be the characteristics of B. kensleyi by the Taiwanese media. To identify the species, we used COI gene of the mitochondrial DNA as the marker. The three samples were collected from Zhengbin Fishing Port in Keelung, Taiwan on 17 July, 2019. According to the information from fishermen, coupled with the GPS record of the fishing vessel involved, these samples were captured in waters of the Pratas Islands about 430 km south-west of Kaohsiung, Taiwan. The genomic DNA was isolated from the pereopods muscle. The primers were designed based on the sequences of the conserved regions of COI. The PCR-amplified products were sequenced using a DNA sequencer. The sequences of COI from the three individuals collected from Pratas Island waters were almost identical to that of B. jamesi collected off Hainan Island, China, in 2017, instead of B. kensleyi. Because both B. kensleyi and B. jamesi have the upwardly curved pleotelsonic spines, molecular biological identification is thought to be essential for their identification.
關鍵字: isopod、 Bathynomus
著作名稱:Phylogenetic analysis of a dajid isopod Holophryxus fusiformis parasitic on the sakura shrimp in Taiwan (台湾産サクラエビに寄生するアミヤドリムシの系統解析)
年度:2020
類別: 會議論文
摘要:[Background] The genus Holophryxus of dajid isopod was established by Richardson in 1905. The H. fusiformis was first discovered in Suruga Bay by Shiino in 1937, but the second record was the one after 80 years in Guishan Island, Yilan, Taiwan. This species is usually yellow and spindle-shaped, parasiting on the carapace of the sakura shrimp Lucensosergia lucens. In order to understand the phylogenetic relationship between H. fusiformis and the other marine isopoda, 18S rRNA gene was cloned and sequenced as the target. [Material & methods] The specimens of sakura shrimp and attached H. fusiformis were collected in Gueishan Island and stored in 70% ethanol at -20 °C. The 18S rRNA gene was with a QIAamp DNA Mini Kit (QIAGEN) and used as a PCR template. The nucleotide sequence obtained was subjected to phylogenetic analysis using Molecular Evolutionary Genetics Analysis 7.0. [Results & discussion] A total of 1200 bases was obtained from the 18S rRNA gene of H. fusiformis. When the sequence was compared with those from 15 species of bopyroid isopods registered on the database of National Center for Biotechnology Information (NCBI), it was found that Zonophryxus quinquedens was the closest to H. fusiformis among the species examined.
關鍵字:Holophryxus fusiformis
著作名稱:cDNA cloning and amino acids structural comparison of tropomyosins from Amur sturgeon and American paddlefish (アムールチョウザメおよびヘラチョウザメ由来トロポミオシンのcDNAコローニングと構造の比較)
年度:2020
類別: 會議論文
摘要: [Background] Acipenseriformes, one group of ancient fish species, has been diverged at the earlier stage of fish evolution. Acipenseriformes is divided into two families, Acipenseridae and Polyodontidae. Between them, there are a few apparent differences in their biological characteristics. In this study, in order to investigate the phylogenetic relationship between them, the amino acid sequences and higher structures of tropomyosins from Amur sturgeon Acipenser schrenckii (Acipenseridae) and American paddlefish Polyodon spathula (Polyodontidae) were examined. [Material & Methods] Live specimens of the two species were provided by Chienhu Cheanchi Ecological Fish Farm, Taipei, Taiwan. RNA was extracted from the fast skeletal muscles. The tropomyosin cDNAs were synthesized with reverse transcriptases. The nucleotide sequences obtained were then deduced to the amino acid sequences by Translate Tool. The comparison in hydrophilicity and hydrophobicity was implemented by ProtScale, whereas the 3D structures were predicted by SWISS-MODEL. Phylogenetic tree based on the amino acid sequence was also drawn by MEGA7. [Results & discussion] Tropomyosins from these two species were 98% identical in the amino acid sequence, and thus showed considerable similarity in hydrophilic and hydrophobic scores, and 3D structure. In the phylogenetic tree, the two species were clearly isolated from the other Osteichthyes species. The results suggest that Acipenseriformes have diverged from the other fish species at the very early stage of evolution.
關鍵字: tropomyosins
著作名稱:The first report on the occurrence of the bopyrid isopod Orbione aristei parasitic on the shrimp Aristeus virilis from Taiwan(台湾産ヒカリチヒロエビにおけるエビヤトリムシOrbione aristei寄生の初報告)
年度:2020
類別: 會議論文
摘要: [Background] Since the first discovery of O. aristei Shiino, 1949, (Crustacea, Isopoda, Bopyridae, Orbioninae) in Japan in 1949, there has been no record of their occurrence. At present, the research on bopyridae in Taiwan is fragmentary. In this study, the current status of bopyrid O. aristei parasitism on A. virilis (Crustacea, Decapoda, Penaeoidea) in Taiwan was investigated by morphological and molecular biological approaches. [Material & methods] A total of 116 individuals of A. virilis purchased at a fish market in Donggang, Taiwan and the parasite sampled therefrom were stored in 70% ethanol and stored at -20 OC until used. The morphology was observed under a dissecting microscope equipped with a CCD system. The 18S rRNA gene was cloned and sequenced for the phylogenetic analysis. [Results & discussion] The parasite was identified as O. aristei based on the following observations. Firstly, the host recorded in this study and the previous studies were A. virilis. Secondly, the female antennule and antenna possessed two and three articles, respectively, and the oostegites completely enclosed the brood pouch, etc. Thirdly, the male antennule and antenna possessed three and four articles, respectively, and the shape of the pleotelson was inverted triangle with a blunt tip. Fourthly, the nucleotide sequence of 18S rRNA gene from O. aristei was clearly different from those of the other bopyrid isopods.
關鍵字:bopyrid isopod
著作名稱:Identification and phylogeny of the bopyrid Parapenaeon tertium as the new host for the shrimp Aristeus virilis (ヒカリチヒロエビに寄生するエビヤドリムシの種同定と系統分類)
年度:2020
類別: 會議論文
摘要:[Background] The bopyridae P. tertium Nierstrasz & Brender à Brandis, 1932, has been known to be parasitic on the shrimp Metapenaeopsis provocatoria longirostris Crosnier, 1987. We found this parasite on a new host shrimp A. virilis, Spence Bate, 1881. The parasitized shrimps suffer from many problems such as stunting and reproductive disorders, causing economic damages to fisheries community. In this study, the bopyrid P. tertium was characterized based on morphological and molecular biological approaches. [Material & methods] Total of 22 individuals of the shrimp A. virilis, with swollen gills were collected on Oct. 9th, 2019 at a fish market in Donggang, Pingtung, Taiwan. The parasites freshly removed manually were soaked in 70% ethanol and kept at -20°C until used. The morphology of the parasites was observed under a dissecting microscope and a scanning electron microscope. The occurrence of parasitism by this bopyrid and its phylogenetic relashionship were examined based on the 18S rRNA gene sequence. [Results & discussion] The shrimps were not similar to the one reported by Boyko (2004). This study is the first report for P. tertium parasitic on the new host A. virilis. The head of the parasite in female individuals was slightly longer and wider than those in males tapering posteriorly, strongly produced with frontal lamina equal to approximately one third of the head in length, and the antenna and antennule consisted of four articles. The barbula of the parasite in the females was one-paired, and the margin of the head was accompanied with numerous irregular lobes. On the other hand, the parasites in the males had six and three articles in the antennae and antennule,
關鍵字:bopyrid
著作名稱:甲骨文の文字にもとづく「龍」の生物学的検証
年度:2020
類別: 會議論文
摘要: [背景]「龍」は中国の重要なシンボルであり、水と密接な関係がある。東漢王朝の許慎《說文》には龍の特徴として「鱗蟲之長。能幽,能明,能細,能巨,能短,能長...」とあり、「龍」が想像上の動物ではないことを示唆する。本研究は、古代において「龍」に相当する動物、その原形となる生物種が魚類である可能性を含めて解明することを目的とした。 [方法]甲骨文にある「龍」の形体と、古代に書かれた文献をもとに「龍」の生物学的特性を分析した。さらに、古代の「龍」と現存生物の特徴を系統分類学的に比較した。 [結果]文献にある「龍」は、近縁生物として、ヘビ、ワニ、トカゲおよびチョウザメの4つに集約された。なかでも、ヘビ型とワニ型がより近縁と考えられた。甲骨文にみる「龍」の特徴は、体が細長くて足がなく、巨大な頭と2本の巨大な牙があり、頭を上げることができることにある。 したがって、足があるワニやトカゲ、巨大な牙がないニシキヘビやチョウザメは該当しないことになる。そのため「龍」の原型は巨大な毒ヘビと考えるのが妥当であり、中国にみられる巨大な毒ヘビの中で大きな頭をもつものとして、コブラが最有力である。すなわち、商王朝以前に「龍」と呼ばれ、現在では中国南部および台湾に分布するタイワンコブラNaja atraである可能性が高いと考えられた。
關鍵字:龍、ワニ、ヘビ、トカゲ、甲骨文、生物学
著作名稱:The “cattle-fish” in the History of The Liao Dynasty (《遼史》における「牛魚」と現存魚種との対応)
年度:2019
類別: 會議論文
摘要:During the Northern Song Dynasty, the northern nomad called Khitan was distributed in the wide range which represents the northeast of China and the broad area in Mongolia nowadays. In 907 A.D, the Liao Dynasty had been founded by Abaoji. According to the descriptions in Miscellany of Yenbei by Wu Gui, “The northern savages hooked cattle-fish in Daru River.” , but little information is available about the species. Regarding the “cattle-fish”, there had been some valuable statements to be compared and verified in the documents over the past dynasties. According to the Record of Yenbei by Wang Yi, “cattle-fish has a long mouth, hard scales, and brittle bones of head weight about a hundred catty. It is so-called the sturgeon in the south,” and “Cattle-fish is a bigger sturgeon.” Fang Yizhi in Tong-Ya told us, “Cattle-fish, is a sturgeon from the north”. It was also considered that cattle-fish was exactly a sturgeon. Compendium of Materia Medica by Li Shizhen quoted the saying of Chen Zhanchi, “Cattle-fish originates in East China Sea, and the shape of its head looks like a cattle,” in which not only sturgeon but also cattle-fish have been mentioned. Therefore, Li thought that both of them were not the same creature. In Fantastic Tales By Ji Xiaolan, Ji narrated, “Cattle-fish, which is so-called kaluga, had been valued in Jin Dynasty, as well as the present.” Apparently there are various explanations for “cattle-fish.” This essay will focus on the History of The Liao Dynasty to boldly expound the distinct creatures or the different species with the same name that “cattle-fish” had referred to in a variety of dynasties and areas by not only researching the related texts with the records of “cattle-fish” since the Liao and the Song Dynasty, but contrasting features of creatures described in the ancient texts. The creatures which had been called as “cattle-fish” might include Psephurus gladius, Huso dauricus, Dugong dugon, Orcinus orca, Silurus glanis and so on; thus, there is a higher possibility that P. gladius might be the “cattle-fish” mentioned in History of The Liao Dynasty.
關鍵字:牛魚
著作名稱:伊藤 洸・○高井則之(日大生物資源)・黄 銘志 (国立台南大学)・飯野祐大・中井静子・糸井史朗 ・杉田治男(日大生物資源)メジナとクロメジナの繁殖戦略:春を待つ メジナ,暖水に向かうクロメジナ
年度:2019
類別: 會議論文
摘要:日本周辺に生息するメジナ科魚類の産卵生態は、メジナGirella punciataについては報告されているがクロメジナGirella leoninaについてはほとんど報告されていない.本研究では,メジナとクロメジナの産卵生態の種間差を明らかにするため,伊豆半島沿岸と種子島沿岸から両種を収集し、生殖腺指数 (GSI)と生殖腺の組織形態に基づき両種の成熟過程を調べた. 種子島海域のメジナは3月に著しく高いGSI値を示しており、伊豆半島海域のピーク時期(4月)より早かった。GSI値の高かったメジナは両海域とも組織学的に成熟した卵巣と精巣を春季に有していたことから,メジナはこれらの海域で春季に産卵することが示唆された. クロメジナのGSIは対照的に低い値を示したが、その最大値は雌雄とも伊豆半島海域より種子島海域で高かった.また,クロメジナの卵形成は伊豆半島海域より種子島海域で進んでいた。この結果から,種子島はクロメジナの産卵海域に比較的近いことが示唆された. 【目的】日本沿岸に分布するメジナ属魚類3種のうち,繁殖生態が詳しく報告されているのはメジナのみであり,他2種の繁殖生態は良く分かっていない.本研究では,漁獲量が多いメジナとクロメジナについて繁殖戦略の種間差を明らかにするため,成熟度分析と着底稚魚の出現状況調査を実施した. 【材料及び方法】台湾近海及び南西諸島沿岸で漁獲されたメジナ属成魚を収集し,生殖腺を摘出して成熟度分析を実施した.また,関東—伊豆地方および九州南部地方の沿岸域でメジナ属の稚魚を採集し,DNA分析に基づいて両種の出現種組成を調べた. 【結果】12–3月に台湾近海で漁獲されたクロメジナから成熟期の卵細胞が検出されたことから,本種は台湾近海の熱帯・亜熱帯海域で冬から早春にかけて産卵しているものと推察された.本種の着底稚魚は,黒潮の流路から離れた相模湾では冬季に全く採集されなかったのに対し,黒潮沿いの相模灘では1–3月に豊富に採集された.トカラ海峡北側の種子島の海岸でも,本種の着底稚魚は3月に採集された.したがって,日本沿岸に来遊する仔稚魚は黒潮流路に近い岩礁底の暖水環境の中で成育すると考えられる.一方,メジナの着底稚魚は相模灘と相模湾の両海域で晩春から初夏にかけて豊富に採集された.メジナの成熟個体は,本州から九州にかけての海域で3–5月に漁獲されることが報告されている.以上の結果から,メジナは「春季昇温依存型」,クロメジナは「暖水海域依存型」の繁殖戦略をとっているものと推察された.
關鍵字:
著作名稱:The biological characteristics of the bopyridae parasitic on the Heterocarpus shrimps (ミノエビ属に寄生するエビヤドリムシの生物学的特徴)
年度:2019
類別: 會議論文
摘要:[Background] The bopyridae Discorsobopyrus stebbingi (Nierstrasz & Brender a Brandis, 1923) is known to parasite on the shrimps Heterocarpus siboage (de Man, 1917) and H. hayashii Crosnier (1988). The shrimps parasitized by this bopyridae suffer from many problems such as stunting and reproductive disorders, causing economic damages to fisheries community. In this study, attempts were made to characterize the parasitism by this species. [Material & methods] The parasite shrimps were collected in a fish market at Pingtung, Donggang, Taiwan. Total of 36 individuals of Heterocarpus shrimps with swollen gills were collected in Sept. 2018, and the parasites freshly removed were soaked in 75% ethanol and kept at -20°C. The morphology was observed under a dissecting microscope and a scanning electron microscope. Molecular biological analysis on the occurrence of parasitism was also performed based on the 18S rRNA sequence. [Results & discussion] The shrimps were identified to be H. hayashii, similar to the one reported by Boyko (2004). It has been observed that the sizes of male and female were so different, namely, the female head was inverted triangle, and the antenna and antennae were only one and hairless. The male had a two-section antenna and one section of tentacles, together with a mid-ventral tubercle. Based on the molecular biology analysis of 18S rRNA, approximately 0.1% of the shrimps examined was found to be parasitized by D. stebbingi.
關鍵字:bopyridae, Discorsobopyrus stebbingi
著作名稱:The morphological observation of the parasitic isopod Epipenaeon ingens ingens Nobilli found in the kuruma prawn Marsupenaeus japonicus in Taiwan (台湾産クルマエビで発見された等脚目寄生虫エビヤトリムシの形態観察)
年度:2019
類別: 會議論文
摘要:[Background] On November 8th, 2018, an individual of kuruma prawn Marsupenaeus japonicus (Spence Bate, 1888) with swelling gills was found at a fish market in Penghu, Taiwan. Attempts were made to identify causal parasite species in this study. [Methods] The morphology of the parasite freshly removed from the host prawn was observed under a stereomicroscope and an optical microscope. [Results & Discussion] It was found that the parasite was more similar to Parapenaeon japonica (Thielemann, 1910) or Epipenaeon ingens ingens (Nobilli, 1906, Crustacea, Isopoda, Bopyridae), but not to Pleurocrypta reported by Ishii in 1916, nor Orbioninae sp. reported by Saito in 2002, It was more likely to be E. ingens ingens based on the characteristics of the five pleomeres, all lateral plates being well developed, and pereon segments being distinct. In this study, E. ingens ingens, that anchored the gill of kuruma prawns, was found from the Sea of Penghu for the first time and approximately 1% of the kuruma prawn in Penghu were parasitized by this species. According to references, prawns parasitized by bopyridae suffer from physiological problems such as stunting and reproductive disorders. Due to the damages to their reproductive functions, these parasites could reduce the population of prawns and affect their resources. The species is to be confirmed by a molecular biology method, and further investigation of its physiological and ecological profiles would favor fishery resource management.
關鍵字:Epipenaeon ingens ingens
著作名稱:《遼史》における「牛魚」と現存魚種との対応
年度:2019
類別: 會議論文
摘要:During the Northern Song Dynasty, the northern nomad called Khitan was distributed in the wide range which represents the northeast of present China and the broad area in Mongolia. In 907 A.D, the Liao Dynasty had been founded by Abaoji. According to the descriptions in Miscellany of Yenbei by Wu Gui, "The northern savages hooked cattlefish in Daru River.", but little information is available about the species. Regarding the "cattle-fish", there had been some valuable statements to be compared and verified in the documents over the past dynasties. According to the Record of Yenbei by Wang Yi, "cattle-fish has a long mouth, hard scales, and brittle bones of head weight about a hundred catty. It is so-called the sturgeon in the south," and "Cattle-fish is a bigger sturgeon." As Fang Yizhi in Tong-Ya wrote, "Cattle-fish, is a sturgeon from the north". It was also considered that cattle-fish was exactly a sturgeon. Compendium of Materia Medica by Li Shizhen quoted the saying of Chen Zhanchi, “Cattle-fish originates in East China Sea, and the shape of its head looks like a cattle," in which not only sturgeon but also cattle-fish have been mentioned. Therefore, Li thought that both of them were not the same creature. In Fantastic Tales by Ji Xiaolan, Ji narrated, "Cattle-fish, which is so-called kaluga, had been valued in Jin Dynasty, as at the present." Apparently there are various explanations for "cattle-fish". This essay will focus on the History of The Liao Dynasty to boldly expound the distinct creatures or the different species with the same name that "cattle-fish" had referred to in a variety of dynasties and areas by not only researching the related texts with the records of "cattle-fish" since the Liao and the Song Dynasty, but contrasting features of creatures described in the ancient texts. The creatures which had been called as "cattle-fish" might include Psephurus gladius, Huso dauricus, Dugong dugon, Orcinus orca, Silurus glanis and so on; thus, there is a higher possibility that P gladius might be the "eattle-fish" mentioned in History of The Liao Dynasty.
關鍵字:牛魚
著作名稱:中国漢時代の「鮪」「鱏」「鱣」の意味と現存魚種との対応
年度:2018
類別: 會議論文
摘要: 「鮪」、「鱏」、「鱣」は古代中国漢『說文解字』の時代から存在する古い文字であるが、時代の流れとともに意味は変わっていった。今は「鮪」が良く使われるが、「鱏」や「鱣」はほとんど使われていない。現在、「鮪」は「まぐろ」と読むが、漢代にはその意味はなかった。本研究は中国古代の文献を元に、現代の分類学に基づいて、古代文学に描写された魚種の同定を試みたものである。  漢の時代以前は「鮪」、「鱏」、「鱣」は現在のどの魚種に相当するのかについて、多くの古代中国の文献における、これらの文字の記載例を分析しながら、生物学的特徴を分類学で調べた。その結果、「鮪」と「鱏」は同じ魚であるが、中国北部では「鮪」と呼び、南部では「鱏」はヘラチョウザメ (学名:Psephurus gladius) と呼ばれる絶滅危惧種であることが判明した。およそ二百年前にはまだ中国各地の大きな河川に生息していた巨大魚であった。その他、「鱣」も中国各地に生存した巨大魚で、現在はカラチョウザメ (Acipenser sinensis) とされている。しかし中国には蒙古、女真などの異民族文化が入り、「鱣」の意味もダウリアチョウ (Huso dauricus) やアムールチョウザメ (A. schrenckii) にまで拡大し、その後、中原のカラチョウザメとダウリアチョウを区別するため、新たに「鰉」の字が誕生した。つまり清時代の後、「鰉」はダウリアチョウザメを意味するようになった。一方、「鮪」は大きい魚という意味であり、シビに繋がると思われる。「鮪」が梶木も意味するのは、「鮪」が中国南部の「鱏」であり,その訓読みがカジキであるためである。日本の遣唐使が中国南部で川に「鱏」を見た時、その特徴的な長い吻から、日本に帰ってカジキを見て「鱏」と勘違いしたのではないかと考えられる。
關鍵字:鮪、鱏、鱣
著作名稱:Variations of tropomyosin molecules from fish fast skeletal muscles as revealed by primary and higher order structures (魚類速筋トロポミオシン分子の一次構造および高次構造からみた多様性)
年度:2018
類別: 會議論文
摘要:[Introduction] Tropomyosin (TM) is an actin-binding protein that is highly conserved throughout evolution. Regarding fish skeletal muscle TMs, the primary structure has been determined for many species, and the occurrence of isoforms has been reported. However, insufficient information is available on the structure of fish muscle TMs so far. In this study, attempts were made to reveal the diversity of fish TMs by structural analyses. [Materials and methods] Live specimens of fishes including Taiwan torrent carp Acrossocheilus paradoxus, round-snout nose Distoechodon tumirostris, Taiwan shoveljaw carp Onychostoma barbatulum and lake candidius dace Candidia barbata) were purchased at a local market in Tainan City, Taiwan. Total RNAs were isolated from fast skeletal muscles, and the first strand cDNAs were synthesized. PCR was subsequently carried out and amplification products were subjected to nucleotide sequencing. Sequence homology search, secondary structure prediction and phylogenetic analysis were performed using the ExPASy bioinformatics tools. The 3-D structure was predicted by Swiss Model Automatic Modelling Mode and DeepView softwares. [Results and discussion] All PCR products having approximately 900 bp contained the CDS nucleotide sequences (1-852) encoding 284 amino acids. These fish TMs were considered to be α-type by comparing with the sequences of TMs so far reported. Their 3-D structures generally resembled each other, but small variations were recognized, probably as a result of environmental adaptation and evolution.
關鍵字:tropomyosin
著作名稱:Availability of muscle tropomyosin as a molecular marker for phylogenetic relationship of fish.
年度:2017
類別: 會議論文
摘要:, .[Introduction] Fish show spcies biodiversity in the strategies to adapt to respective environments. Many attempts have been made to clarify the genetic relationships based on some molecular markers such as mitochondrial cytochrome b (cytb) and cytochrome c oxidase subunit I (COI) genes. Not all of them are, however, successful in these attempts. Muscle tropomyosin (TM) is one of the regulatory proteins of muscle contraction, and its structure is characterized by a typical coiled-coil formed by conservative amino acid sequences. In this study, the availability of TM as a new molecular marker for phylogenetic analysis of fish was evaluated, by taking the advantage of highly conserved features of TM sequences. [Methods] The DNA and deduced amino acid sequences of TM, cytb and COI from white croaker (Pennahia argentata), bluefin tuna (Thunnus orientalis), pufferfish (Takifugu rubripes), zebrafish (Danio rerio), Atlantic salmon (Salmo salar) were used. The phylogenetic analyses were performed by using different analytical methods such as maximum likelihood, neighbor joining and UPGMA. [Results and discussion] The phylogenetic trees drawn based on TM sequences were similar to those constructed by using traditional markers. The primary, secondary, and the modelled 3-D structures of TMs were similar to each other, but distinguishable among the five species. Such differences in the structure imply the involvement of TM for adaptation of fish concerned to each habitat. It is suggested that TM can be an excellent molecular marker for the phylogenesis of fish and would give valuable crews to their evolution.
關鍵字: tropomyosin, fish
著作名稱:Possibility of myoglobin as a molecular marker for phylogenetic relationship of fish.
年度:2017
類別: 會議論文
摘要:.[Introduction] Fish show a large biodiversity in the strategies to adapt to respective inhabiting environments. In order to know the genetic relationship among fish species, many attempts have been made based on various molecular markers such as mitochondrial cytochrome b (cytb) and cytochrome c oxidase subunit I (COI). Not all of them are, however, successful for this elucidation. In the previous study, we found the availability of muscle tropomyosin (TM) as a good molecular marker. In this study, myoglobin (Mb) was evaluated as a possible alternative molecular marker for the elucidation of phylogenetic relationship of fish. [Methods] Publicly available data of the DNA and deduced amino acid sequences of Mb, cytb and COI were obtained for longtooth grouper (Epinephelus bruneus), medaka (Oryzias latipes), whale shark (Rhincodon typus), bluefin tuna (Thunnus thynnus), green spotted puffer (Tetraodon nigroviridis), torafugu (Takifugu rubripes), zebrafish (Danio rerio), Atlantic salmon (Salmo salar) and tilapia (Oreochromis mossambicus). The phylogenetic analyses were performed based on the different methods namely, maximum likelihood, neighbor joining and UPGMA. [Results and discussion] The phylogenetic trees depicted based on Mb sequences were similar to those based on the traditional classification markers. The primary, secondary, and the modelled 3-D structures of Mbs were similar among Mbs from different fish species, but were clearly distinguishable among the nine species. Such differences in the structures would be responsible for adaptation of Mb molecules to the physiological conditions of each species. These results suggest that Mb can be a molecular marker for the phylogenesis of fish, but is slightly inferior to TM. Fish show great biodiversity in the strategies to adapt to respective inhabiting environments. In order to know the genetic relationship among fish species, many attempts have been made to identify and classify the genetic relationship based on some mol ecular markers such as mitochondrial cytochrome b (c yt b and cytochrome c oxidase subunit I COI ) genes. Not all of them are, however, successful for this purpose. In the previous study, we found the availab ility of muscle tropomyosin ( for this purpos e (Yu and Huang, 2016)2016). In this study, the possibility of myoglobin as a new molecular marker for the above purpose classification and phylogenetic analysis of fish was evaluated.
關鍵字:myoglobin
著作名稱:東港產太平洋黑鮪(Thunnus orientalis)骨骼肌庫鬥蟲研究
年度:2017
類別: 會議論文
摘要: 庫鬥蟲是黏液胞子蟲類的寄生蟲,被寄生的太平洋黑鮪(Thunnus orientalis)在被捕獲後引發肌肉溶解症。肌肉溶解現象對漁民造成經濟損失。這種寄生蟲一旦被攝入人體會引發嚴重食物中毒症狀如腹瀉和嘔吐。誤食者症狀通常出現在2-20小時後,症狀有時很嚴重但在排出庫鬥蟲後通常在24小時內恢復。本研究蒐集屏東東港魚市販售的10個黑鮪幼體樣本,平均重量和長度分別為1.85公斤,全長 42.5厘米,尾叉長40厘米。採集後的黑鮪幼體儲在-20°C冰箱,次日切割魚體橫截面,確認庫鬥蟲之存在後再使用PCR方法鑑定庫鬥蟲種類。目前已找到黑鮪肌肉被庫鬥蟲寄生之現象並特定庫鬥蟲種類。這研究將提高台灣人民對生魚消費如壽司和生魚片的警覺性,因為病原蟲就在當中。
關鍵字:黑鮪, 庫鬥蟲
著作名稱:台灣太平洋黑鮪熟肉分析及探討
年度:2016
類別: 會議論文
摘要:
關鍵字:
著作名稱:台湾近海産のクロマグロで発生したヤケ肉の生化学特徴につきまして
年度:2016
類別: 會議論文
摘要:[目的] ヤケ肉は異常肉の一種でマグロ類に多く見られ,その発生による経済的損失が大きい。ヤケ肉の特徴は淡い筋肉色,若干の酸味と不快臭,保水性低下によるテクスチュアの劣化である。本研究では台湾産クロマグロに見られたヤケ肉の生化学的特性を明らかにすることを目的とした。 [方法] 2015年4月~6月、台湾東港で水揚げされた生鮮クロマグロ15個体からヤケ肉および正常肉を採取し、pH、乳酸含量、色彩値(L *, a *, b *値)および抗酸化酵素スーパーオキシドジスムターゼ(SOD)、グルタチオンレダクターゼ(GR) 、カタラーゼ(CAT)の活性を調べた。さらに、チオバルビツール酸反応物質(TBARS)および抗酸化活性の指標として2,2-ジフェニル-1-ピクリルヒドラジル(DPPH)ラジカル消去活性を測定した。 [結果] pH値はヤケ肉および正常肉でそれぞれ5.57±0.1および5.95±0.1と前者で有意に低かった(P0.001)。乳酸含量はヤケ肉および正常肉でそれぞれ2.12±0.24および1.69±0.28 mg/g前者で高い傾向を示した。また、L*値はそれぞれ39.4​​±3.7および30.1±4.6。a *値で有意差が認められなかったが、b *値はそれぞれ13.1±2.8および8.2±3.4とヤケ肉の方が高い値を示した。抗酸化酵素活性についてはSODではそれぞれ11.1±1.2および 12.5±1.3 U/mg of prot、 GRではそれぞれ5.1±1.7および6.6±1.9 U/g of prot 、CATではそれぞれ0.88±0.1および0.95±0.11 U/mg of protとヤケ肉で低かった。一方、TBARS値は、それぞれ14.1±4.3および9.3±3.0 nmol /gとヤケ肉で高かったが、DPPH濃度については両者で有意差は認められなかった。以上の結果は、マグロが漁獲時に暴れると筋肉に乳酸が蓄積してpHの低下、肉色の劣化が生じるだけではなく、抗酸化酵素の活性も低下し、ヤケ肉の進行一因となることを示唆する。
關鍵字:ヤケ肉、タイヘイヨウクロマグロ、台湾、肉質、抗酸化酵素
著作名稱:2016日本水產學會:台湾近海産のクロマグロで発生したヤケ肉の生化学特徴につきまして
年度:2016
類別: 會議論文
摘要:[目的] ヤケ肉は異常肉の一種でマグロ類に多く見られ,その発生による経済的損失が大きい。ヤケ肉の特徴は淡い筋肉色,若干の酸味と不快臭,保水性低下によるテクスチュアの劣化である。本研究では台湾産クロマグロに見られたヤケ肉の生化学的特性を明らかにすることを目的とした。 [方法] 2015年4月~6月、台湾東港で水揚げされた生鮮クロマグロ15個体からヤケ肉および正常肉を採取し、pH、乳酸含量、色彩値(L *, a *, b *値)および抗酸化酵素スーパーオキシドジスムターゼ(SOD)、グルタチオンレダクターゼ(GR) 、カタラーゼ(CAT)の活性を調べた。さらに、チオバルビツール酸反応物質(TBARS)および抗酸化活性の指標として2,2-ジフェニル-1-ピクリルヒドラジル(DPPH)ラジカル消去活性を測定した。 [結果] pH値はヤケ肉および正常肉でそれぞれ5.57±0.1および5.95±0.1と前者で有意に低かった(P0.001)。乳酸含量はヤケ肉および正常肉でそれぞれ2.12±0.24および1.69±0.28 mg/g前者で高い傾向を示した。また、L*値はそれぞれ39.4​​±3.7および30.1±4.6。a *値で有意差が認められなかったが、b *値はそれぞれ13.1±2.8および8.2±3.4とヤケ肉の方が高い値を示した。抗酸化酵素活性についてはSODではそれぞれ11.1±1.2および 12.5±1.3 U/mg of prot、 GRではそれぞれ5.1±1.7および6.6±1.9 U/g of prot 、CATではそれぞれ0.88±0.1および0.95±0.11 U/mg of protとヤケ肉で低かった。一方、TBARS値は、それぞれ14.1±4.3および9.3±3.0 nmol /gとヤケ肉で高かったが、DPPH濃度については両者で有意差は認められなかった。以上の結果は、マグロが漁獲時に暴れると筋肉に乳酸が蓄積してpHの低下、肉色の劣化が生じるだけではなく、抗酸化酵素の活性も低下し、ヤケ肉の進行一因となることを示唆する。
關鍵字:ヤケ肉、タイヘイヨウクロマグロ、台湾、肉質、抗酸化酵素
著作名稱:Primary structure and structure simulation of tropomyosin from fast skeletal muscle of Onychostoma barbatulum (モロコ速筋トロポミオシンの一次構造解析と立体構造予測)
年度:2016
類別: 會議論文
摘要:Tropomyosins form a famtily of actin-binding proteins whose structures have been highly conserved throughout evolution. Tropomyosin is an excellent model protein which can predict the stability of myofibrillar proteins. The Onychostoma barbatulum , one of Cyprinidae species, belongs to the primary freshwater fish with a pan-island distribution, in Taiwan. The species is regarded important one, since its meat is known to be delicious, and thus the cultured amount has been increasing in recent years. In this study, attempts were made to reveal the structural characteristics of dudgeon tropomyosin.
關鍵字:tropomyosin
著作名稱:黒潮流域のけるメジナ属の再生産機構
年度:2016
類別: 會議論文
摘要:
關鍵字:黑潮、メジナ属
著作名稱: Gene cloning and thermodynamic property of muscle tropomyosin from milkfish (Chanos chanos)
年度:2015
類別: 會議論文
摘要:[Background] Milkfish is one of the popular aquaculture species in Southeast Asia. In winter, especially after the cold current passes through Taiwan, many milkfish cannot survive because of low water temperatures. It has become a serious problem for aquaculture industry and gives economic damage for fishermen. The reason, however, has still remained unclear. While some other tropical species such as mudskipper, grouper and tilapia are also farmed, they hardly die unlike milkfish. In this study, the thermodynamic properties of tropomyosin from these species were compared to address the question. [Materials and methods] The nucleotide sequences of the tropomyosin genes were determined by cDNA cloning, and their amino acid sequences were deduced. Fast skeletal muscle tropomyosins were isolated by a combination of isoelectric point precipitation and salting out with ammonium sulfate. The thermodynamic properties of the purified tropomyosins were examined by differential scanning calorimetry (DSC) and circular dichroism (CD) spectrometry. [Results and discussion] The results showed that the deduced amino acid sequences of tropomyosins from the above species were similar to each other, with the identity being 96-98%. Thermodynamic analysis showed that the milkfish tropomyosin has lower refolding ability after heating up to 80℃, compared to those of the other species. It is still unclear whether such low stability of milkfish tropomyosin is directly related to the lower tolerance of this species against cold water, but could support the technical approach to this question. [目的]台湾でサバヒー(虱目魚、学名 Chanos chanos )と呼ぶミルクフィッシュは、東南アジアで重要な養殖魚類であり、練り品など様々加工食品の原料とも使われており、しかし独特の特徴を持ち、品質の安定性は低いと 言われた。今研究は、原料及び加工品質を高めるため、筋肉繊維の一つトロポミオシンを用いて、一次構造及び熱安定性の解析を行いました。 [方法]台湾産ミルクフィッシュ普通筋を用いて、RNAからcDNAを合成した。既報他種魚類筋肉トロポミオシンの相同性の高い配列に基づいて作成した数種プライマーを用いて、トロポミオシン全長をコードするcDNA クローンを得た。GOR及びSWISS-MODELを用いて、演繹アミノ酸配列を元に二次構造および三次構造の予測を行った。さらにミルクフィッシュ普通筋由来のアセトンパウダーを用いて、遠心分離、同電点沈殿、硫安分化 により純化した普通筋トロポミオシンを得た。純化した普通筋トロポミオシンを用いて、二次元電気泳動、CD及びDSCなど熱安定性試験を行った。 [結果]ミルクフィッシュ筋肉トロポミオシンcDNAクローニングに成功した。852の塩基配列を決めた、284演繹アミノ残基を得た。既報他種魚類筋肉トロポミオシンアミノ酸残基と94%以上高い同一率を示した。 Head-to-tail 重合に必要なN末端の12残基及びC末端の8塩基、さらに2つのサブユニットの結合に重要なCys190は保存されていた。GOR及びSWISS-MODEL構造予測により、ほぼ全体は􀀀- へリックス構造を示した。二 次元電気泳動の結果から、普通筋は一種類のトロポミオシンを同定した。CD分析の結果、ミルクフィッシュ筋肉トロポミオシンは他種魚類と同じ222及び208 nm におい極小を示す、􀀀- へリックスに特異的なパターンが 認められた。二回目測定により、加熱、冷却後のrefoldingが不完全である事を示唆した。DSC分析の結果、筋肉トロポミオシン転移温度(Tm)は47.9℃のことを示した。
關鍵字: milkfish
著作名稱:太平洋黑鮪(Thunnus orientalis)熟肉可能發生原因調查
年度:2014
類別: 會議論文
摘要: 熟肉(burnt meat) 是異常肉的一種,常可在黑鮪肌肉發生,台灣東海岸在四月至七月常可補到太平洋黑鮪 (Thunnus orientalis),特別是在六、七月熟肉發生機率明顯增加,夏季較高的水溫被懷疑是造成里郭內之可能原因。雖然黑鮪熟肉現象在台灣發生已有相當時間,但是發生之原因及機制仍未被了解,本研究室經初步之生化分析顯示肌肉中有數種氨基酸分解,及酸度增加。,為更了解其原因及機制,我們嘗試了解東港整個黑鮪產業作業流程,嘗試找出可能產生黑鮪熟肉之原因。希望能降低漁業損失,增加海洋資源之利用。 由於目前並非黑鮪產季,本調查為研究之前置分析,目的在網羅發生黑鮪熟 肉之所有可能因子,作為日後研究之方向,並以訪談之方式做調查。訪談期間為 2013年9月至12月,訪談之對象以黑鮪漁業相關業者為主,包含船長、漁船作 業員、黑鮪肢解業者、黑鮪買賣業者,生魚片業者、餐廳業者、行政管理者等。 訪談內容包含黑鮪生態、捕捞方式、處理方式、保存方法,運方式,產生熟肉之可能原因等。 經訪談黑鮪相關業者後發現,可能產生黑鯛熟肉問題之原因相當多元化。高 水溫與處理不良被認為是發生黑鮪熟肉有最大可能性,其餘如死亡時間。 肌肉油脂量,死前掙扎。溫度管理、 黑鮪個性、黑鮪品系、潮汐、日月週期等均有 相當之關聯性。將以此調查資作研究之方向,於黑鮪產季時持續觀察及求證相關可能發生黑鮪熟肉因子。 在海洋資源逐漸枯竭之際,希望能找出鮪類發生熟肉之原因,降低漁業損失, 讓海洋資源能夠發揮最大效應。
關鍵字:熟肉、太平洋黑鮪、訪談、台灣
著作名稱:Gene cloning and structure analysis of muscle tropomyosin from tilapia subspecies, Oreochromis niloticus, O mossambica and a hybrid O.mossambica X O.niloticus.
年度:2014
類別: 會議論文
摘要:Tilapias are common aquaculture species in Taiwan. The most common species are Oreochromis niloticus, O. mossambica and a hybrid O. mossambica (♂) × O. niloticus (♀)). Tropomyosin is a fibrous protein involved in muscle contraction regulation, and is also responsible for the quality of muscle foods. This protein is also of great interest from the viewpoint of molecular evolution and the structure-function relationship. In order to reveal the structural profiles of tropomyosin from different strained tilapias, we investigated the characteristics of skeletal muscle tropomyosin composition for the above tilapias based on gene cloning.Total RNA isolated from dorsal skeletal muscle from tilapias was utilized as the templates for PCR amplification and sequencing of cDNA encoding muscle tropomyosin using the gene-specific primers. The cDNA was cloned into the bacterial expression vector pGEM-T Easy and the protein was expressed in E. coli strain of JM109 cells. The sequences were edited and analyzed by BioEdit program. The results obtained showed that the open reading frames for all the tropomyosin genes contained 852 bp encoding 284 amino acid residues. Tropomyosin genes from the three tilapias had slightly different nucleotide sequences coding the same amino acid sequence. The deduced amino acid sequence showed a considerably high homology in a range of 94-99% to those of the other fish α-type tropomyosins reported so far. By the phylogenetic analysis, tropomyosin from the hybrid tilapia showed the closest relationship with that from nilotica species O. niloticus.
關鍵字:tilapia
著作名稱: Heavy metal and trace element concentrations in the muscles of five species from the Dong Gang Coast, Taiwan
年度:2014
類別: 會議論文
摘要:[Objective] The sea beds of west Taiwan mostly belong to the continental shelf. The Dong Gang Coast is the only one place deeper than 200 meters, with unique ecosystem and biodiversity. In the present study, attempts were made to measure the concentrations of heavy metals and trace elements in the muscles of fish species inhabiting the waters in order to examine the effect of water pollution in the waters concerned in inhabiting fish species. [Methods] Three teleost fishes, namely, horned lanternfish Diaphus splendidus, Japanese barracudina Lestrolepis japonica, and kamohara grenadier Coelorinchus kamoharai, and two elasmobranch species, blue shark Prionace glauca and silky shark Carcharhinus falciformis, were collected in a local fish market in Dong Gang. The five species of fish were very common species and fresh specimens are available in this area. The specimens were stored at -20℃ until used. The dorsal ordinary muscles were excised and minced with a mincer and were dried in an oven during 8 h. Two hundred milligrams of dried powders was applied to a Microwave Digestion System. The inductively coupled plasma-reactive ion etching (ICP-RIE) was used to determine the contents of Mn, Fe, Ni, Cu, Zn, Cd, Pb, Se, Cr, Ge, Sn, Hg, and As in the samples. [Results and discussion] The most of the elements were found in fish muscles except Sn. Although Pb was not detected in the teleost fish species, the two shark species gave the value higher than 2,000 ppm. Fe, Mn, Ge, As, Zn, and Cd in the teleost fish were higher than in the sharks. Ni content in shark muscles was higher than in the teleost fishes. On the other hand, the contents of Cr, Hg, and Cu were similar to each other among the above teleosts and sharks. The results demonstrated that the sharks as high level predators had more concentrated levels of heavy metals than those of lower levels. The bioaccumulation of these elements seems to have taken place along Dong Gang Coast. It is suggested that some elements such as Hg and Cr have originated from industrial pollution.
關鍵字:Heavy metal
著作名稱:2013日本水產學會-頭足類外套膜筋トロポミオシンの精製条件の検討
年度:2013
類別: 會議論文
摘要:トロポミオシン(TM)は骨格筋や心筋の細いフィラメント上に存在する、約33 kDaのサブユニットのダイマーで、筋収縮の調節に関与している タンパク質である。分子は棒状で、ほぼ全長にわたり特有のコイルドコイル構造をとっている(図1、2)。我々のグループは既に、寒帯から亜熱帯に 生息する魚類の速筋から精製したTMについて、アミノ酸配列と熱安定性との関係を明らかにしてきた。軟体類のTMは、高等動物由来のものとは異 なる性質を示すという報告は多いが、精製後の性状を調べた例はあまりない。また、精製条件は脊椎動物TMのものを、そのまま適用できないことが 知られている。本研究では、温度適応と頭足類TMの安定性との関連を明らかにするために、台湾周辺の亜熱帯海域に生息する代表的な種類を対 象に、外套膜筋TMの精製条件について検討を行なった。
關鍵字:トロポミオシン
著作名稱:吳冠毅、陳瑩是、黃銘志。香葉天竺葵、迷迭香、薄荷等草本植物驅蚊效果之研究
年度:2012
類別: 會議論文
摘要:台灣位處熱帶、亞熱帶。古來即為瘧疾、登革熱等熱帶蚊蟲媒介疾病盛行區域。近年來醫藥衛生的發達與普及,已經大為降低蚊蟲媒介疾病之盛行率。然而過度使用化學性藥劑滅蚊之結果,也造成生態嚴重之破壞及失衡。目前國際社會盛行有機天然方式代替人工化學方式,這種風氣不但在病蟲害防治、土壤改良等技術外,在防治蚊蟲上也逐漸被重視。天然植物中有些天然除蟲成分,利用這些特性,降低環境之蚊蟲量,如何讓來園的遊客在炎炎夏日中享受園區的景觀設備而不被蚊蟲叮咬,是許多主題樂園、花園重要的課題之一。 本研究採用坊間盛傳具有除蟲效果之草本植物-香葉天竺葵(Pelargonium graveolens)、迷迭香(Rosmarinus officinalis)、薄荷(Mentha spicata)作為研究對象,分析並比較這三種植物的驅蚊效果之成效。為能適用於休閒遊樂區之應用,採用實際樣區研究方式。研究方法如下:選定一基地分為實驗區與對照區,栽種不同實驗植物並以相同方式之捕捉法與計量方式,看蚊蟲族群種類數量及消長方式。經由數學模式之統計分析三種驅蚊植物之效用。 由初步成果顯示,香葉天竺葵(Pelargonium graveolens)、迷迭香(Rosmarinus officinalis)、薄荷(Mentha spicata)均有驅蚊之效果,至於何者成效較佳,什麼物質具驅蚊效果則需進一步分析討論。 希望研究成果對於蚊蟲防制有所助益,並減少熱帶蚊蟲媒介疾病之盛行率,讓大家有個安全又快樂的夏天。
關鍵字:天竺葵、迷迭香、薄荷
著作名稱:2012日本水產大會-Characterization of crustacean muscle tropomyosins
年度:2012
類別: 會議論文
摘要:Crabs and shrimps have been popular eatables and important aquaculture species in Taiwan. Those crustaceans have distinctive flavors and high protein contents as good meats. The characteristics of muscle proteins are known to be greatly different to fish species. Among them, we have been working on fish muscle tropomyosins (TMs), revealing their species-specific differences in stability. Invertebrate TMs are known to be clearly different from vertebrate ones. Crustaceans TMs were seem as a common antigen for man. The amino acid sequences of TMs were different among species, so it shown the various polarity and charge-containing. However, it was found to be hard to purify invertebrate TMs by the conventional method for fish TMs. TMs from the black tiger prawn Penaeus monodon, giant freshwater prawn Macrobrachium rosenbergii, mangrove crab Scylla serrata and red spotted swimming crab Portunus sanguinolentus caught along the coast of Taiwan were purified by the combination of ammonium sulphate fractionation, continuous- and step-wise ion-exchange chromatography and dialytic precipitation. The results were shown that the TMs from crustaceans were different in molecular weights (33~37 kDa) and affinity to anion-exchange resins. This implies that the proteins were difference just for limited amino acids displaced. The 2D-PAGEs were shown Crustaceans TMs with a major spot and might not have isoform TM in muscle. It was easy eluted by potassium chloride solution from 165mM~ 215mM. We have further explored optimal conditions for the purification of TMs.
關鍵字:Tropomyosin
著作名稱:大彈塗魚肌肉蛋白tropomyosin cDNA clone 之研究
年度:2011
類別: 會議論文
摘要:原肌球蛋白 (tropomyosin, TM) 是一種與肌鈣蛋白 (troponin) 形成肌鈣蛋白複合體的蛋白質,藉由與肌動蛋白 (actin) 的結合來調節肌肉之收縮,原肌球蛋白是由兩股α-螺旋的多胜肽鏈纏繞而成的結構。大彈塗魚 (Boleophthalmus pectinirostris) 屬鰕虎科 (Gobiidae) 魚種,分佈於台灣北部及西部沙地沿岸,雜食性,為重要之經濟性食用魚類。本實驗由魚體上取骨骼肌,抽取total RNA,利用反轉錄聚合反應可得原肌球蛋白的cDNA,再使用聚合連鎖反應 (PCR) 放大該片段,經定序儀可定序出原肌球蛋白之核苷酸序列,並推測可能胺基酸序列。由已知種類得知原肌球蛋白的分子量大約為33,000 Da,由284個胺基酸組成,其N端與C端的胺基酸序列有高度保留的現象。原肌球蛋白的研究有助於了解肌肉收縮能力在不同種上之異同,亦可作為判斷種間的親原性遠近之指標。
關鍵字:大彈塗魚、原肌球蛋白、cDNA clone
著作名稱:劉育涵、黃銘志。 吳郭魚原肌凝蛋白質特性及熱穩定性研究 BP-19, p236
年度:2009
類別: 會議論文
摘要:
關鍵字:吳郭魚
著作名稱:日本43界癲癇學會-Mutation screening of GABRG2 gene in some types of epilepsy patients.
年度:2009
類別: 會議論文
摘要:Missense mutations in the GABAA receptor gamma2 subunits (GABRG2) gene have been detected in patients with typical absence seizures and generalized epilepsy with febrile seizures plus. We performed mutation analysis of all the coding exons of GABRG2 genes by direct sequencing to identify missense mutations in much wider range of epilepsy patients. A total of 94 Japanese patients including febrile seizure, generalized epilepsies, generalized epilepsy with febrile seizures plus, severe myoclonic epilepsy in infancy, partial epilepsies, and west syndrome were screening for the nine exons and the relevant exon–intron boundaries of GABRG2 gene. One nosynonymous mutation in exon 2 and three single nucleotide polymorphisms (SNPs) in exon 3, exon 5 and exon 9, respectively, were identied in our analysis. In conclusion, mutations in GABRG2 do not seem to be a major genetic cause of epilepsy in our subjects. Key word: epilepsy, GABRG2, single nucleotide polymorphisms (SNPs)
關鍵字:
著作名稱:Ming-Chih Huang.(2009) The study of tropomyosins from tropical fish muscle. "Seminar Invitation for Laboratories of Marine Biochemistry, the University of Tokyo" July 11-13, 2009 in Chiba, Japan.
年度:2009
類別: 會議論文
摘要:
關鍵字:
著作名稱:Toshio Kojima, Yuto Ueda, Aya Kitamoto, Akira Sato, Naoki Adati, Ming-Chih Huang. (2009) Gene expression profiling of a rat model of neonatal hypoxic-ischemic encepharopathy using DNA microarray. The Japan Neuroscience Society. September 16- 18, Nagoya, Japan
年度:2009
類別: 會議論文
摘要:
關鍵字:
著作名稱:Ming-Chih Huang, Shuichi Yoshida, Motohiro Okada, Kohtaro Morita, Hiroshi Nagafuji, Shinichi Hirose, Sunao Kaneko, and Toshio Kojima. (2009) Mutation screening of GABRG2 gene in some types of epilepsy patients. The 43rd Congress of the Japan epilepsy society, international symposium. October 24, 2009, Hirosaki, Japan.
年度:2009
類別: 會議論文
摘要:
關鍵字:
著作名稱:Ming-Chih Huang, Motohiro Okada, Fubito Nakatsu, Hirokazu Oguni, Masatoshi Ito, Kohtaro Morita, Hiroshi Nagafuji, Shinichi Hirose, Sunao Kaneko, Hiroshi Ohno, Yoshiyuki Sakaki and Toshio Kojima.  Mutation Screening of AP3M2 in Japanese Epilepsy Patients. Human Genome Organization (HUGO) Human Genome Meeting (HGM2007) in Montreal, May 20-24, 2007. Canada
年度:2007
類別: 會議論文
摘要:
關鍵字:
著作名稱:鮪類品系鑑定研究
年度:2022
類別: 會議論文
摘要:
關鍵字:鮪、品系
著作名稱:台灣東沙島海域捕獲的巨型深海等足類大王具足蟲分子生物學分析
年度:2022
類別: 會議論文
摘要: Bathynomus species are deep-sea crustaceans distributed in the deep ocean floor of the Pacific, Atlantic and Indian Oceans. According to the references, there are at least three species of Bathynomus in the South China Sea, including B. doederleini, B. kensleyi and B. jamesi. The differences between the species of genus Bathynomus are very small and not easily distinguished based on the morphology (Fig. 1, Table 1). The upwardly curved pleotelsonic spines are often considered to be the characteristics of B. kensleyi by the Taiwanese media. To identify the species, we used COI gene of the mitochondrial DNA as the marker. The three samples were collected from Zhengbin Fishing Port in Keelung, Taiwan on 17 July, 2019. According to the information from fishermen, coupled with the GPS record of the fishing vessel involved, these samples were captured in waters of the Pratas Islands about 430 km south-west of Kaohsiung, Taiwan. The genomic DNA was isolated from the pereopods muscle. The primers were designed based on the sequences of the conserved regions of COI. The PCR-amplified products were sequenced using a DNA sequencer. The sequences of COI from the three individuals collected from Pratas Island waters were almost identical to that of B. jamesi collected off Hainan Island, China, in 2017, instead of B. kensleyi. Because both B. kensleyi and B. jamesi have the upwardly curved pleotelsonic spines, molecular biological identification is thought to be essential for their identification.
關鍵字:東沙島
著作名稱:海神後海螯蝦Metanephrops neptunus (Bruce, 1965)之型態觀察
年度:2021
類別: 會議論文
摘要:海神後海螯蝦Metanephrops neptunus (Bruce, 1965)屬於後海螯蝦屬(Metanephrops)蝦種,於1965年在南中國海域首次發現,漁民稱其為「金絲猴」或「紅頭龍蝦」,在澳洲則被稱為尼普頓龍蝦(Neptune lobster)。 本實驗樣本於2019年7月採自宜蘭正濱漁港之漁民,共計有三隻雌性與七隻雄性個體,全長介於23~28公分,重量介於272~505公克之間,測量後進行拍照及蝦體外觀描繪。 觀察結果發現雌、雄個體擁有相近的顏色–紅色的頭胸甲和乳白色腹節。在胃中發現底棲多毛綱動物、線蟲類和甲殼類殘渣。在頭胸甲上有均勻的小刺以及在與額後龍骨瓣(postrostral carinae)之間有較大的刺狀凸起為此種分類關鍵。本次發現之海神後海螯蝦為台灣新紀錄種,為東沙群島漁業資源之一,期望可作為管理漁業資源的參考。
關鍵字:海神後海螯蝦
著作名稱:利用原肌球蛋白做為分子標記探討魚類系統發育
年度:2021
類別: 會議論文
摘要:[目的]在脊椎動物中魚類的種類佔了很大的比例,因此魚類的分類及系統發育研究便是一個重要的課題,本研究希望以原肌球蛋白( tropomyosin, TM )作為分子標記,探討魚類的系統發育與原肌球蛋白之間的關係。 [方法]本實驗自美國國家生物技術資訊中心 (National Center for Biotechnology Information, NCBI) 取90種不同魚類之原肌球蛋白,透過BioEdit軟體比對胺基酸序列,並使用MEGA X軟體的Neighbor-Joining法繪製演化樹探討魚類的系統發育。 [結果] 經BioEdit軟體比對後發現於第24、27、35、63、111、135、172及175號位點的氨基酸,在骨鯡群(Otomorpha)及原棘鰭亞群(Protacanthopterygii)魚類有產生單一胺基酸變異,將Neighbor-Joining法繪製的演化樹與Ricardo Betancur-R等人於2017年所提出的硬骨魚類系統發生樹相比,有一定程度的相似,由此可以推斷魚類原肌球蛋白一種適合用來探討魚類系統發育的分子標記。
關鍵字:分子標記、系統發育
著作名稱:東港櫻花蝦(Lucensosergia lucens)混獲生物種類調查
年度:2020
類別: 會議論文
摘要:櫻花蝦(Sergia lucens)是東港重要的產業之一,與黑鮪魚、油魚子被稱為東港三寶。櫻花蝦是珍貴之資源,在日本是極為昂貴之水產,國人常曬乾食用。捕撈時期為每年之十一月至翌年五月,漁期可達半年之久。捕捉櫻花蝦目前以小型底拖網為主,而且櫻花蝦體型精小鈴攏,捕撈之漁網網目也相當細小,所以大小魚類乃至於各種仔稚魚也不放過,對海洋生態之危害甚大,雖然對外宣稱有自我管理與調節機制,但年年下降之櫻花蝦漁獲量卻是此項資源嚴重之隱憂。捕撈櫻花蝦產生之混獲問題也極為嚴重,卻未被社會大眾關心過,混獲之下雜魚可能遠比櫻花蝦之產量為大,種類包羅萬象甚至有許多新種之出現。最常見的種類包含各種類之燈籠魚、深海鯊魚、底鱈、甲殼類等。本研究調查櫻花蝦產季採集櫻花蝦混獲之生物種類,希望在櫻花蝦產業崩潰之前留下一份混獲魚種之資料,更希望能有更多學生、關懷海洋人士參與此議題,並喚起大眾對於海洋資源之珍惜與重視。
關鍵字:東港、櫻花蝦、混獲、種類調查
著作名稱:東港產思氏不對稱鰓虱Discorsobopyrus stebbingi(Nierstrasz & Brender à Brandis, 1923)的生物特性分析
年度:2020
類別: 會議論文
摘要:
關鍵字:思氏不對稱鰓虱
著作名稱:「龍」字之古文字學生物考證
年度:2020
類別: 會議論文
摘要:「龍」在中國是重要的象徵。東漢許慎在《說文》曾經解釋龍為「鱗蟲之長。能幽,能明,能細,能巨,能短,能長;春分而登天,秋分而潛淵」。 《左傳》記載:「昔叔安,有裔子曰董父,實甚好龍,能求其耆欲以飲食之,龍多歸之。」可知龍並非虛幻之物。由「龍」字來看,左半邊為頭部,右半邊為身體,屬於象形文字。古代人民所指的龍是什麼呢?而龍的原型是什麼生物呢?為瞭解開此謎題,研究中國最早古文字所記錄之生物特徵是一個重要的線索。 利用古文字學字型,分析其生物特徵。並利用生物分類學之基礎,將古人記載「龍」字的特徵與現今生物做比對。 根據中國古籍所記述「龍」的特徵,歸納出最接近之四種生物為蛇、鱷、蜥蜴及鱘魚。目前學界普遍認為龍的原型以「蛇型說」和「鱷型說」最為有力,兩派學說均有合理解釋。根據已解析所知甲骨文「龍」字所傳達生物特徴如下:身體呈長條形、無足、頭部巨大、擁有二巨牙、頭部能昂起等特點,排除了鱷魚、蜥蜴(無足)和蟒蛇、鱘魚(無巨牙)。顯然巨大毒蛇是「龍」的原型,在中國是巨大毒蛇又同時具有膨大頭部特徵者,眼鏡王蛇是最可能的種類。中國在商朝以前稱呼「龍」,極有可能是現今生長於中國南方的眼鏡王蛇。
關鍵字:生物考證
著作名稱:圓吻鯝( Distoechodon tumirostris ) 原肌球蛋白( Tropomyosin ) 基因克隆( cloning ) 與結構分析
年度:2020
類別: 會議論文
摘要:[目的]圓吻鯝 ( Distoechodon tumirostris ) 屬鯉科圓吻鯝屬 ( Distoechodon ) 魚種,分布於台灣北部及中國南部,主要棲息在淡水河川與湖泊中下層水域,但繁殖季時會溯溪產卵。由於是河流性淡水魚類,本研究希望透過將圓吻鯝調節肌肉收縮的原肌球蛋白( Tropomyosin, TM )與生活於激流與緩流之其他鯉科魚類比較,藉以探討環境適應對圓吻鯝肌肉演化的影響。 [方法]本實驗取魚背部骨骼肌,抽取total RNA,利用反轉錄聚合反應獲取TM之cDNA,再使用聚合酶連鎖反應放大片段,送定序儀定序核苷酸序列,並預測其演繹胺基酸序列。並使用已定序之核苷酸及胺基酸序列,利用MEGA軟體分析與其他魚類之親緣關係。 [結果]本研究定序圓吻鯝肌肉TM共855個核苷酸序列,演繹胺基酸為284個,結果相似於其他魚類背部肌肉TM胺基酸數量。將此序列與其他已知魚類TM序列做比對後發現:圓吻鯝與生活於激流的台灣石魚賓( Acrossocheilus paradoxus )、台灣白甲魚 ( Onychostoma barbatulum ) 和生活於緩流的斑馬魚 ( Danio rerio )、鯽魚(Carassius auratus) 等魚類肌肉相比,圓吻鯝TM與激流魚類較為相似。推測其原因可能是圓吻鯝為了適應河川激流及溯溪繁殖演化而成,雖然圓吻鯝平時生活於緩流但其TM結構與分佈於激流的鯉科魚類較為接近。此結果應證了環境因子會影響魚類表徵之理論。
關鍵字:圓吻鯝、原肌球蛋白
著作名稱:宜蘭龜山島產梭形附頭虱Holophryxus fusiformis Shiino, 1937之顯微觀察
年度:2019
類別: 會議論文
摘要:梭形附頭蝨於1937年在日本首次發現。80年後,在龜山島第二次發現,主要寄生於正櫻蝦Lucensosergia lucens(Hansen, 1922)之頭胸甲上方。附頭蝨採集於龜山島根據顯微鏡觀察,附頭蝨雌蟲外型差異性甚大,顏色隨卵成熟度改變,有些肥胖,呈卵圓形;或呈香腸型。體色由黃到橙都有。雄蟲變異性大,特別是尾部;有柱狀、圓形或倒三角形。此研究希望了解附頭蝨外形差異性外,也希望了解寄生蟲對櫻花蝦危害,作為管理櫻花蝦資源之參考。
關鍵字:梭形附頭虱
著作名稱:東港太平洋黑鮪肌肉之庫道蟲種類鑑定與顯微觀察
年度:2019
類別: 會議論文
摘要:庫道蟲(Kudoa)是黏液胞子蟲類的微生物,目前已知會寄生在魚類的肌肉上。被寄生的太平洋黑鮪(Thunnus orientalis)在被捕獲後可能引發肌肉溶解症,對漁業造成損失。這種微生物一旦被攝入人體可能引發嚴重食物中毒症狀如腹瀉和嘔吐。台灣在庫道蟲的文獻較少,國人對此類微生物的認識較為不足。本研究蒐集屏東東港漁市販售的黑鮪魚幼體樣本並儲存在 -20°C冰箱。取尾部肌肉做微生物分析並用顯微鏡做型態觀察,初步鑑定為Kudoa hexapunctata。目前已找到黑鮪肌肉有庫道蟲寄生之現象,本次研究以鑑定台灣海域黑鮪肌肉上庫道蟲種類為主要目的。
關鍵字:太平洋黑鮪、庫道蟲
著作名稱:屏東東港產林氏異腕蝦Heterocarpus hayashii Corsnier, 1988寄生蟲Discorsobopyrus stebbingi Nierstrasz & Brandis, 1923之鑑定與顯微觀察
年度:2019
類別: 會議論文
摘要: 蝦類寄生Discorsobopyrus stebbingi  (Nierstrasz & Brender à Brandis, 1923)在文獻中主要寄生在東方異腕蝦(Heterocarpus siboage de Man,1917)鰓蓋上。2018年9月12日在東港的林氏異腕蝦上也發現疑似同種的寄生蟲,可能成為此寄生蟲的新宿主。根據解剖顯微鏡及電子顯微鏡觀察,外型特徵幾乎與 Boyko, 2004所發現的相同,寄生蟲雌雄都呈白色,雌性寄生蟲身體呈傾斜、頭部呈倒三角狀、兩對觸角皆呈一段等特徵顯示在外觀無太大差異。雄性則因為樣本都尚未成熟無法進行判斷。東方和林氏異腕蝦雖然形態上極為相似,但可依前後兩根背刺長的比例與體色及斑點位置判斷。 林氏異腕蝦在東港是重要的漁業資源之一,本研究希望了解異腕蝦與寄生蟲之間的關係,及其對蝦類的危害,作為管理漁業資源的參考。
關鍵字:林氏異腕蝦
著作名稱:彈塗魚的求偶行為和生態習性
年度:2018
類別: 會議論文
摘要:彈塗魚是常見於河口及溪流泥灘地和溼地的魚類,在環境中是許多鳥類的食物來 源,同時也能當作是辨別環境好壞的生態指標,但是對於彈塗魚的很多生態行為並沒 有太多的解釋。本篇在於探討彈塗魚求偶行為與生態習性,一般人的認知中,大彈塗 魚的求偶行為就是彈跳起來。為了要觀察大彈塗魚的求偶行為,時間選在 6月中正值彈塗魚求偶期,地點選在高雄茄萣白砂崙濕地,我們利用攝影機在每次乾潮前一小時, 以及滿潮前一小時架在岸邊拍攝2個小時至3個小時。觀察結果發現彈跳只是所有求偶 行為中的一部分而已,而求偶行為大致上可以分為三部分,第一部分是彈跳、第二部 分是擺尾,最後則是引導,每個動作都有其意義。而我們本次的結果在高雄茄萣的白 砂崙濕地,同時也發現了青彈塗魚的蹤跡,青彈塗魚在水位高時,出現的頻率較多, 所以推論青彈塗魚對水的依賴程度比大彈塗魚還要高,對溫度的耐受性比大彈塗魚還 要低。大彈塗魚的求偶行為經研究後,比一開始認知的還要複雜,而台灣青彈塗魚的 發現紀錄大多是在新北市的挖子尾自然保留區,南部相對較少,大彈塗魚的外觀與青 彈塗魚極為相似,而唯一最大的不同在於第一背鰭的外觀,大彈塗魚的第一背鰭是扇 形,青彈塗魚的第一背鰭則是旗桿狀,所以不仔細看是無法分辨其差異。
關鍵字:大彈塗魚、青彈塗魚、求偶行為、白沙崙濕地
著作名稱:《詩經》中的「鱣」、「鱏」、「鮪」物種考證
年度:2016
類別: 會議論文
摘要:《詩經》是中國最早的詩歌總集及純文學專著,收錄了西周初到春秋中期(西元前一千年至西元前四百年)約六百年間的文學作品。詩詞忠實呈現當時人民的生活方式、觀念與民間習俗,所以是研究漢族文化與風俗重要參考著作。 《詩經》中記載許多生物種類,其中三分之一以上內容提到植物,反映了當時漢族環境與可利用的生物資源。而魚的記述與人民的生活、宗廟祭祀息息相關,《詩經》中也常提及,其中有關大魚的記述常出現「鱣」、「鱏」、「鮪」等魚類。漢族在遷徙擴張的過程中,受到地域、方言、傳抄及錯誤解釋下產生許多異名或錯誤。為了瞭解古代《詩經》魚類現今學名,本研究根據《詩經》及注疏所描述之生物特徵作為魚類分類學之依據,除確立今日學名外,也將探討分布範圍。 經《詩經》及注疏所描述魚類特徵分析顯示;「鱣」、「鱏」、「鮪」均是當時出產於黃河流域江河中的大魚,以今日學名而言,「鱣」為中華鱘(Acipenser sinensis),而「鱏」和「鮪」所描述特徵均指向白鱘(Psephurus gladius)。根據古文獻記載,白鱘和中華鱘曾分布在廣大的漢人活動區,然而今日上述兩種大魚均成為瀕臨絕種之珍稀魚類。這些曾和漢民族祖先有相當接觸的魚類,現在的我們已不再熟悉它,白鱘可能已經絕滅。 《詩經》中將「鱣」和「鱏」分辨得很清楚,是兩種外型結構截然不同的魚類。然而今人卻將「鱣」和「鱏」混為同一種「鱘」類。建議中華鱘應該正名為中華鱣,而今日常用的「鱘」即是古字「鱏」,就是指白鱘一種魚類。本論文目的在確認古文中的魚類,還原並瞭解漢人生活中利用這些魚類的歷史。
關鍵字:契丹、遼史、牛魚、白鱘、達氏鰉
著作名稱:《遼史》中的牛魚考證
年度:2016
類別: 會議論文
摘要:北宋年間,北方的游牧民族契丹分布於今中國東北及蒙古廣大地區,於西元907年由耶律阿保基建立遼王朝。遼與宋之間由於環境及民族的不同,產生許多文化差異。《遼史》記載遼國君主常在初春河川上「鉤魚」,遼國君主鉤什麼魚?宋‧武珪《燕北雜錄》中記述:「北虜於達魯河鉤牛魚。」北虜指蒙古人,達魯河為今東北吉林省扶余縣松花江、嫩江交接處,而「牛魚」是指什麼魚呢? 歷史文獻中,有數種關於牛魚究竟是何魚的討論。宋‧王易《燕北錄》云「牛魚觜長鱗硬,頭有脆骨重百斤,即南方鱏魚也。」又云「牛魚即鱘之大者。」明‧方以智《通雅》「牛魚,北方之鮪類也。」也認為是鱏魚。明‧李時珍《本草綱目》引陳藏器「牛魚生東海,其頭似牛」,既有鱘魚,又載牛魚,所以李時珍認為鱘魚和牛魚並非同種生物。清‧紀曉嵐《閱草堂筆記》「金重牛魚,即瀋陽鱘鰉魚,今尚重之。」可見牛魚一詞有不同的解釋。 本文考察遼、宋以後有關牛魚記載的文本,並對照古代文獻描述的生物特徵,大膽推測「牛魚」一詞在不同的朝代及地域指不同生物,屬於異物同名的例子。曾在歷史上被稱為「牛魚」的生物可能包含白鱘(Psephurus gladius)、達氏鰉(Huso dauricus)、儒艮(Dugong dugon)、虎鯨(Orcinus orca) 、六鬚鯰魚(Silurus glanis)等。《遼史》所指牛魚,以白鱘(Psephurus gladius)之可能性最高。本文在通識課程中,能結合教學與研究,進行人文社會科學與自然科學的對話。 在北宋年間,契丹是北方的游牧民族,分布在今中國東北及蒙古廣大地區。契丹族在西元907年由耶律阿保基建立遼王朝。由於環境及民族不同,遼與宋有許多不同的文化。其中在《遼史》記載遼國君主常在初春河川上「鈎魚」。遼國君主鈎什麼魚?在宋武珪《燕北雜錄》中記述;「北虜於達魯河鈎牛魚。」北虜指蒙古人,達魯河為今東北吉林省扶余縣松花江、嫩江交接處。宋程大昌《演繁露》也記載「達魯河鈎牛魚,遼地盛禮。意慕中國賞花釣魚然,非釣也鈎也。」而「牛魚」是指什麼魚呢? 在推測牛魚可能的魚類之同時,首先對遼國君主鈎牛魚之目的需作一些說明。契丹人是游牧民族,對於打獵漁撈相當重視。頭鵝宴及頭魚宴及是皇帝重視漁獵知識的表徵,有如以農立國的漢族君主祭祀五穀之神的涵意。頭魚宴如何舉行?契丹皇帝常會在春來江河解凍之前在群臣護衛下到春捺缽地先鑿冰鉤魚,之後再縱鷹鶻捕雁鵝。頭魚是一年中第一次獲得的魚,顯得極為貴重,再由君主設酒宴宴請全臣,為契丹族的盛禮。此頭魚一定是貴重而大的魚,由於是大魚,無法用釣魚的方式,所以是用鉤的。根據脫脫《遼史》記載,遼國君主鉤魚的地點最常在土河(老哈河)、鴨子河(松花江)、混同江(第二松花江)等地。這些區域有哪些大魚且是高貴可進貢給皇上的呢?許多文獻清楚的紀錄這種魚類稱之為牛魚。 歷史上針對牛魚有過各種描述;宋李昉《太平御覽》卷九三九鱗介部引《臨海異物志》云:「牛魚,形如犢子,毛色青黃。好眠臥,人臨其上,及覺,聲如大牛,聞一里。」三國魏武《四時食制》曰:「海牛魚皮、生毛可以飾物,出揚州。」晉張華《博物志》曰:「東海中有牛魚,形如牛。剝其皮懸之,潮水至則毛起,潮去則伏。」宋王易《燕北錄》云「牛魚觜長鱗硬,頭有脆骨重百斤,即南方鱏魚也。」又云「牛魚即鱘之大者。」宋周必大《二老堂雜誌》「賛寧《物類相感志》引《博物志》云:「東海有牛魚,其形似牛,剝其皮懸之,潮水至則毛起,退則毛伏。今東牟有海牛島,其牛無角,足似龜,長丈餘,尾若鮎云。」明李時珍《本草綱目》引陳藏器曰「牛魚生東海,其頭似牛。」以上之敘述,可以發現對牛魚有不同之詮釋。 本研究主要蒐集遼宋以前有關牛魚記載的文本,並對照古文描述之生物特徵,推測「牛魚」一詞在不同的時期及地域指不同生物。在宋朝以前漢族描述的牛魚以海牛類之海中哺乳動物為主,宋朝以後北方契丹人建立遼國後,漢人受到遼人習慣影響,也將漢族習慣稱為鱏的江中大魚稱為牛魚。曾在宋朝以前被稱為「牛魚」的生物可能包含白鱘(Psephurus gladius)、達氏鰉(Huso dauricus)、儒艮(Dugong dugon)等。而《遼史》所指牛魚,以白鱘(Psephurus gladius)之可能性最高。
關鍵字:牛魚
著作名稱:論清代曹廷杰《西伯利亞東偏紀要》中的麻特勒魚
年度:2016
類別: 會議論文
摘要:
關鍵字:
著作名稱:台灣石魚賓(Acrossocheilus paradoxus) 原肌球蛋白基因克隆與結構分析
年度:2016
類別: 會議論文
摘要:原肌球蛋白(tropomyosin,TM)為一種與肌鈣蛋白(troponin)形成肌鈣蛋白複合體的蛋白質,藉由與肌動蛋白(actin)的結合來調節肌肉之收縮。原肌球蛋白是由兩股α-螺旋的多胜肽鏈纏繞而成的結構。臺灣石魚賓(Acrossocheilus paradoxus)屬光唇魚屬(Acrossocheilus),體側具7條黑色橫帶,幼魚較成魚明顯,為台灣特有種魚類,普遍分布於台灣河川溪流中。由於熱帶魚類原肌球蛋白肌肉特性研究較為缺乏,臺灣石魚賓為台灣特有種類,對於研究肌肉結構及與其他魚種作比對有相當的研究價值,此次以肌肉結構中較易研究之原肌球蛋白作為探討之對象。自花蓮XX溪中捕捉野生石魚賓,從活體取魚背部的骨骼肌,抽取total RNA,利用反轉錄聚合反應獲取原肌球蛋白之cDNA,再使用聚合酶連鎖反應(PCR)放大片段,最後送入定序儀定序出核苷酸及演繹胺基酸序列,利用蛋白質模擬系統推測其蛋白質立體結構。由結果得知臺灣石魚賓原肌球蛋白其分子量約33000Da,284個胺基酸序列,N端和C端序列皆有高度保留的現象。原肌球蛋白的研究有助於比較不同物種肌肉運動上的差異,也可藉由其基因序列上的差異來進行物種親源的研究。
關鍵字:臺灣石魚賓、肌肉、原肌球蛋白、立體結構、親緣關係
著作名稱:甲殼類原肌球蛋白純化之探討
年度:2016
類別: 會議論文
摘要:原肌凝蛋白 (Tropomyosin, TM) 廣泛存在於動物肌肉與細胞骨架中,在肌肉收縮與細胞內物質移動上扮演重要的角色。多年來許多研究指出TM蛋白是甲殼類中主要的過敏原,解開TM蛋白的過敏致病機轉有助於減少過敏症狀發生,而純化出高純度的TM蛋白有助於TM蛋白物理化學性質的研究。傳統對於魚類TM蛋白的純化方式並無法有效從組織中分離出TM蛋白,甲殼類肌肉蛋白有不同的化學性質且TM蛋白分子量也較大。本研究結果顯示出,蝦類TM蛋白可利用30% 硫酸銨分畫分離出TM粗蛋白,蟹類則是在26%處有較好的分離效果。陰離子交換樹脂管柱層析結果顯示,白蝦非TM蛋白在沖提液濃度90 mM KCl處分離,TM蛋白在135 mM KCl處分離。紅星梭子蟹 (Portunus Sanguinolentus) 及鋸緣青蟹 (Scylla Serrata) 的非TM蛋白隨著flow through分離,TM蛋白則在215 mM KCl處分離。此外本實驗嘗試利用透析沉澱法分離白蝦TM蛋白及非TM蛋白,取得初步的成果,值得深入研究。
關鍵字:甲殼類、原肌凝蛋白、蛋白質抽取
著作名稱:論清代文獻中的牛魚
年度:2016
類別: 會議論文
摘要:《遼史》記載遼國君主常在初春河上「鈎魚」,武珪《燕北雜錄》中記述:「北虜於達魯河鈎牛魚」,歷代文獻關於「牛魚」有不同的解釋。王易《燕北錄》云「牛魚觜長鱗硬,頭有脆骨重百斤,即南方鱏魚也」、「牛魚即鱘之大者」。方以智《通雅》說「牛魚,北方之鮪類也。」也認為是鱏魚。李時珍《本草綱目》曰「牛魚生東海,其頭似牛」提及鱘魚和牛魚,兩者並非同種生物。紀曉嵐《閱微草堂筆記》說「金重牛魚,即瀋陽鱘鰉魚,今尚重之。」。「牛魚」是什麼呢?本文聚焦於清代文獻有關「牛魚」的文本,曾被稱為「牛魚」的生物可能包含白鱘(Psephurus gladius)、達氏鰉(Huso dauricus)、儒艮(Dugong dugon)、六鬚鯰魚(Silurus glanis)、虎鯨(Orcinus orca)及鬼蝠魟(Manta birostris)等,清代的「牛魚」很可能是達氏鰉以及白鱘。
關鍵字:牛魚
著作名稱:魚翅及鯊魚種類利用探討
年度:2016
類別: 會議論文
摘要:
關鍵字:鯊魚種類
著作名稱:鮪類骨骼特徵分析之初步探討
年度:2016
類別: 會議論文
摘要:
關鍵字:鮪類骨骼
著作名稱:四帶雞魚原肌球蛋白基因克隆與結構分析
年度:2015
類別: 會議論文
摘要:
關鍵字:四帶雞魚
著作名稱:太平洋黑鮪(Thunnus orientalis)熟肉之生化學分析
年度:2015
類別: 會議論文
摘要:熟肉 (burnt meat)是一種異常肉,常發生在鮪類等大型魚類肌肉。「熟肉」顧名思義就是像煮熟過的肉,肉色顯得較亮白,口感缺乏彈性,味道比正常肉呈現酸性。台灣四月至七月為捕捉太平洋黑鮪 (Thunnus orientalis) 之季節,端午節之後,黑鮪熟肉發生率增加,造成漁民莫大損失。然而黑鮪熟肉發生之機制至今未明,問題也未被解決。本研究嘗試比較熟肉與正常肉之生化特性。於2014 年五月至七月蒐集東港黑鮪熟肉樣本三十件,經初步實驗分析結果如下;肌肉組織酸度增加(熟肉5.56±0.1,正常肉5.97±0.1),顏色變得較蒼白 (L值熟肉39.34±3.6,正常肉30.03±4.5)。利用15% SDS-PAGE 電泳發現熟肉在50 KDa 有蛋白質降解。另作肌肉組織之TBARS 值實驗顯示熟肉過氧化程度比起正常肉來的高 (31.27±19.79 vs. 9.41±7.17)。本研究顯示熟肉現象包含許多蛋白質分解過程,與溫度、酸鹼度及時間有關連,將進一步模擬熟肉發生之機制。
關鍵字:太平洋黑鮪
著作名稱:太平洋黑鮪(Thunnus orientalis)熟肉現象之初步生化學分析
年度:2015
類別: 會議論文
摘要:熟肉(burnt meat)是一種異常肉,常發生在鮪類等大型魚類肌肉。「熟肉」顧名思義就是像煮熟過的肉,肉色顯得較亮白,口感缺乏彈性,味道比正常肉呈現酸性。台灣四月至七月為捕捉太平洋黑鮪(Thunnus orientalis)之季節,端午節之後,黑鮪熟肉發生率增加,造成漁民莫大損失。然黑鮪熟肉發生之機制至今未明,問題也未被解決。本研究嘗試比較熟肉與正常肉之生化特性。於2014年五月至七月蒐集東港黑鮪熟肉樣本十五件,經初步實驗分析結果如下;肌肉組織酸度增加(熟肉5.56± ,正常肉5.97± ),顏色變得較蒼白(L值熟肉39.34± ,正常肉30.03± )。利用15% SDS-PAGE電泳發現熟肉在50 KDa 有蛋白質降解。另作肌肉組織之TBARS值實驗顯示熟肉過氧化程度比起正常肉來的高。 本研究顯示熟肉現象包含許多蛋白質分解過程,與溫度、酸鹼度及時間有關連,將進一步模擬熟肉發生之機制。
關鍵字:太平洋黑鮪
著作名稱:亮眶燈魚(Diaphus splendidus)、日本光鱗魚(Lestrolepis japonica)、蒲原氏腔吻鱈(Coelorinchus kamoharai)三種深海魚類體內元素分析
年度:2014
類別: 會議論文
摘要:每年逢十一月至隔年六月為台東縣東港鎮捕撈櫻花蝦(Sergia lucens)的季節,由於櫻花蝦(Sergia lucens)位於深海之下,加上其細小的身形,漁民需以緊密細小的網子並採用底拖網的方式捕撈,每逢捕撈櫻花蝦的同時,伴隨上岸的往往是一些較低經濟價值的生物,包括魚類、蝦類、頭足類等,也就是俗稱的「下雜魚」,我們針對亮眶燈魚(Diaphus splendidus)、日本光鱗魚(Lestrolepis japonica)、白帶魚(Trichiurus lepturus)這三種常見的下雜魚,對牠們體內一些特殊的元素進行檢測及定量,間接了解深海底下蘊藏著哪些能量。
關鍵字:亮眶燈魚
著作名稱:東港地區魚翅來源及鯊魚種類初探
年度:2014
類別: 會議論文
摘要:鯊魚在海洋生態中扮演高層掠食者的角色,如同陸地上獅、虎等地位,具有穩定海洋生態、維持物種多樣化之功能。由於鯊魚之生態特殊,繁殖後代個體數少,族群回復之能力相對較弱。然而國人嗜食魚翅,婚慶宴席更少不了魚翅,加深了鯊魚被人類濫捕之命運。美國最大的魚翅消費市場加州已於2012年1月1日起禁止販售魚翅,其目的在於維護海洋生態與生物之多樣性。東港是台灣重要之鯊魚卸貨漁港,在臺灣有重要之地位。本調查在了解東港地區鯊魚利用之情形,了解魚翅使用鯊魚之種類、學名、地方名與魚翅名等基本資料,希望能作為了解此一產業之依據,並希望藉此傳達鯊魚生態之重要性,維護海洋生態。
關鍵字:魚翅
著作名稱:東港櫻花蝦(Sergia lucens)產業混獲生物種類初步調查
年度:2014
類別: 會議論文
摘要:櫻花蝦是東港重要的產業之一,與黑鮪魚、油魚子被稱為東港三寶。櫻花蝦是珍貴之資源,在日本是極為昂貴之水產,國人常曬乾食用。捕撈時期為每年之十一月至翌年五月,漁期可達半年之久。捕捉櫻花蝦目前以小型底拖網為主,而且櫻花蝦體型甚小,捕撈之漁網網目也相當細小,所以大小魚類乃至於各種仔稚魚也不放過,對海洋生態之危害甚大,雖然對外宣稱有自我管理與調節機制。但年年下降之櫻花蝦漁獲量卻是此項資源嚴重之隱憂。捕撈櫻花蝦產生之混獲問題也極為嚴重,卻未被社會大眾關心過,混獲之下雜魚可能遠比櫻花蝦之產量為大,種類包羅萬象甚至有許多新種之出現。最常見的種類包含各種類之燈籠魚、深海鯊魚、底鱈、頭足類、甲殼類等。本調查在記錄櫻花蝦產業混獲之種類,希望在櫻花蝦產業崩潰之前留下一份混獲魚種之資料,更希望能有更多學生參予此議題,並喚起大眾對於海洋資源之珍惜與重視。
關鍵字:櫻花蝦
著作名稱:由原肌球蛋白tropomyosin探討鱸形目Perciformes魚類之親緣關係
年度:2014
類別: 會議論文
摘要: 魚類是脊椎動物中種類最多的一個族群,而鱸形目 Perciformes)魚類更是硬骨魚類中最大的一個分群,其具有許多種類不同但外型相當接近的魚類,且在基因的表現上也相當的多元化, 因此物種鑑定及分類的研究變成一個重要的課題。 原肌球蛋白(tropomyosin, TM)是一種廣泛存在於各種生物的蛋白,其蛋白質 N端及C端具有相當的保守性,且從原始的微生物到脊椎動物均可發現它的存 在。動物的 TM 最常見於肌肉中的纖維,其扮演著與肌動蛋白(actin)、肌凝蛋白 (myosin)相互協調並促進肌肉調節與收縮之重要角色。由於TM具有高度保守並 廣泛存在高、低等生物肌肉中的特性,因此有作為分子標記的可行性。目前TM 在魚類物種鑑定及分類上的研究相當稀少,且多以不同目之魚類作較廣範圍的研 究,因此本研究嘗試以TM蛋白及DNA作為分子標記,利用資料庫中已登錄肌肉 TM 胺基酸及DNA序列之鱸形目魚類,以計算(calculation)與模擬(simulation) 之系統生物學方法,分析鱸形目中不同魚類親緣關係的遠近,探討生物差異性在生態及適應上之意義。 由分析結果發現無論在DNA或蛋白質序列上皆有相異之處,可能與演化及環境適應有相當程度的關聯。本研究希望能以TM 為觀點,在物種鑑定及分類的研究中建立一個新的指標。
關鍵字:原肌球蛋白
著作名稱:砂蛸 (Octopus aegina) 肌肉原肌球蛋白之純化與分析
年度:2014
類別: 會議論文
摘要:砂蛸(Octopus degina)屬於軟體動物門頭足綱八腕目章魚科章魚屬,分布在台灣,日本 及中國沿海大陸棚,是一種主要的海洋經濟物種。在脊椎和無脊椎動物中,原肌球蛋白 (tropomyosin, TM 為一廣泛存在於肌肉的纖维蛋白,分子量 33kDa,由 284 個胺基酸殘基组成,结構是兩條alfa-helix 以 coiled coil 交互纏繞形成;原肌球蛋白和肌钙蛋白 (troponin)間會形成複合體,與肌動蛋白(actin)和肌凝蛋白(myosin)共同調控肌肉收缩。本次研究目標為確認砂蛸之 TM 蛋白纯化條件,以丙酮處理肌肉組織並製成丙酮乾粉末,利用等電點沉澱、高速離心、硫酸銨分劃和透析法等步驟精製 TM,再以蛋白質電泳觀察 TM 分佈狀況。結果呈現在50-60%的硫胺濃度下 TM 分離的效果最佳。後續將微差掃瞄熱卡計 Differential scanning calorimetry, DSC)和圓二色光譜儀 (Circular dichroism. CD) 分析 TM 的熱穩定性。期望對於軟體動物肌肉生理方面的研究 有所幫助。
關鍵字:原肌球蛋白、砂蛸、高速離心、硫酸銨分劃、蛋白質電泳
著作名稱:彈塗魚-台灣生態保育的指標生物
年度:2013
類別: 會議論文
摘要:
關鍵字:彈塗魚
著作名稱:台江國家公園彈塗魚生態研究
年度:2013
類別: 會議論文
摘要:
關鍵字:彈塗魚
著作名稱:Study of the properties of sea crabs’ tropomyosin and purification condition
年度:2011
類別: 會議論文
摘要:Tropomyosins (TMs) are not only found in muscle fibers but also in non-muscle cells. They are wildly existence in eukaryotes. TMs are composed of two subunits (approximately 33 kDa), and forms a coiled-coil structure which consisting of two parallel α-helical polypeptides. They are associating with myosin complex and actin on the thin filaments of striated muscle. Some references describe human allergic reaction is caused by crab’s TMs. In this study, three crabs like mangrove crab (Scylla serrata), blue swimmer crab (Portunus pelagicus) and corab crab (Charybdis feriata) for the samples. We use high speed centrifugation, ammonium sulfate saturation and isoelectric point to find the best purified condition of crab’s TMs. On the next step, we will use the TMs and try to look for mechanism of human allergic reaction caused by carb’s TMs.
關鍵字:tropomyosin, protein purification, crabs.
著作名稱:Assay and purification of skeletal muscle tropomyosin from small abalone (Haliotis diversicolor)
年度:2011
類別: 會議論文
摘要:Small abalone (Haliotis diversicolor) is a species of marine gastropod mollusk in the family Haliotidae and the genus Haliotis. It has widespread distribution, include coastal waters of Boso Peninsula, Kyushu, Korean Penin-sula, south China and Taiwan. It is an important organism for fishery in Taiwan. Tropomyosin (TM) is widely distributed in all cell types asso- ciated with actin as a fibrous molecule composed of two α-helical chains arranged as a coiled-coil. Molecule mass of TM in the muscle cell is 33 kDa and containing 284 amino acids, it can regulate muscle contraction by interaction with actin and myosin. The perpous of this study is to confirm the method of purifi-cation of TM. We used ultracentrifugation, isoele- ctric point, and salting out with ammonium sulfate to extracting the crude TM from small abalone skeletal muscle. Then we purified TM by column chromatography and detected its cha-racteristics by electrophoresis gel analysis. We hope this study can be useful for protein structure study.
關鍵字:Small abalone
著作名稱:利用模擬系統分析蝦類過敏原-原肌球蛋白(tropomyosin)之結構差異
年度:2011
類別: 會議論文
摘要:部分人食用蝦類可能會引起過敏反應,文獻指出其過敏原可能是存在於肌肉中的原肌球蛋白 (tropomyosin, TM) 。原肌球蛋白是一種普遍存在於動物肌肉中的蛋白,與肌動蛋白 (actin)、肌球蛋白 (myosin) 共同協調肌肉之收縮,其基本結構由兩個α-helix以coiled-coil方式形成。為了解蝦類引發人類過敏反應之機制,本研究分析資料庫已知生物種原肌球蛋白胺基酸序列做比對,並透過電腦模擬技術解釋易產生過敏之原因,及引發過敏反應之機制。希望此研究有助於了解蝦類原肌球蛋白引發過敏之原因,減少過敏發生,造福人群。
關鍵字:蝦類、過敏原、原肌球蛋白、模擬系統、蛋白質結構
著作名稱:利用原肌球蛋白tropomyosin探討魚類演化關係
年度:2011
類別: 會議論文
摘要:原肌球蛋白 (tropomyosin, TM) 是一種存在於肌肉中的纖維蛋白。藉由與肌動蛋白 (actin)、肌凝蛋白 (myosin) 之協調促進肌肉之調節與收縮。有別於肌動蛋白與肌凝蛋白的高分子量,肌肉中的TM以284個胺基酸殘基 為主,分子量約33 kDa。目前之研究發現:原肌球蛋白是一種保守性相當高的纖維蛋白,動物肌肉中均可發現原肌球蛋白之存在。利用原肌球蛋白高度保守性及分子量小之特性,本研究嘗試以原肌球蛋白作為標記,說 明生物演化之相關位置。魚類出現於泥盆紀 (Devonian),由原始的無頜魚類 (Agnatha) 演化出盾皮魚類 (Placodermi) 後再發展出軟骨魚,再變成今日常見的高等硬骨魚類。本實驗將以數種不同現生魚類作為研究對象,利 用資料庫中已知肌肉TM胺基酸序列之魚類,利用計算與模擬 (simulation) 之系統生物學方法,探討親源關係及差異在生態上之意義。由魚類肌肉胺基酸一次結構分析得知,序列第27、135及其他數個胺基酸隨著魚種不同 有些許之差異,推測與演化的先後有相當程度的關聯。希望利用原肌球蛋白技術能夠以不同觀點解釋生物演化先後的關係,並可作為判斷物種親緣性的指標。
關鍵字:原肌球蛋白、硬骨魚、魚類演化、系統生物學
著作名稱: Study for purification methods of muscle tropomyosin from cephalopoda - Octopus vulgaris.
年度:2011
類別: 會議論文
摘要:Tropomyosin (TM) is a kind of muscle protein which wildly exists in eukaryotes, associating with troponin to form a complex and then binding with actin to regulate muscle contraction. Currently, the molecular weight of TM in vertebrates like human, rabbits, and fish is known near to approximately 33 kDa, and consists of 284 amino acid residues. In recent study, it is found out that TM in invertebrates is allergen of food allergy and most of the studies are about crustaceans such as crabs and shrimps; however, there are less studies regarding other invertebrates. In this study, we choose a species of cephalopoda - octopus (Octopus vulgaris) as our sample, which is one of invertebrates to understand the biochemical features of muscle TM so that we can compare with vertebrates. In this study we refer to known purification conditions of TM. We use some biochemical methods like high-speed centrifugation, ammonium sulfate saturation and isoelectric point to purify the cephalopodous TM. We wish this study can benefit to food-processing and medical fields.
關鍵字:tropomyosin, cephalopoda, octopus, protein purification
著作名稱:尤御丞、陳俊文、黃銘志。利用原肌球蛋白tropomyosin探討魚類演化關係
年度:2011
類別: 會議論文
摘要: 原肌球蛋白(tropomyosin, TM)是一種存在於肌肉中的纖維蛋白。藉由與肌動蛋白(actin)、肌凝蛋白(myosin)之協調促進肌肉之調節與收縮。有別於肌動蛋白與肌凝蛋白的高分子量,肌肉中的TM以284個胺基酸殘基為主,分子量約33kDa。目前之研究發現:原肌球蛋白是一種保守性相當高的纖維蛋白,動物肌肉中均可發現原肌凝蛋白之存在。利用原肌球蛋白高度保守性及分子量小之特性,本研究嘗試以原肌球蛋白作為標記,說明生物演化之相關位置。 魚類出現於泥盆紀(Devonian),由原始的無頜魚類(Agnatha)演化出盾皮魚類(Placodermi)後再發展出軟骨魚,再變成今日常見的高等硬骨魚類。本實驗將以數種不同現生魚類作為研究對象,利用資料庫中已知肌肉TM胺基酸序列之魚類,利用計算與模擬(simulation)之系統生物學方法,探討親源關係及差異在生態上之意義。由魚類肌肉胺基酸一次結構分析得知,序列第27、135及其他數個胺基酸隨著魚種不同有些許之差異,推測與演化的先後有相當程度的關聯。希望利用原肌球蛋白技術能夠以不同觀點解釋生物演化先後的關係,並可作為判斷物種親緣性的指標。
關鍵字:肌球蛋白
著作名稱:Structural diversity of tropomyosins from different strain tilapia muscle.
年度:2010
類別: 會議論文
摘要:吳郭魚是台灣重要的養殖魚種之一,每年為我國賺取大量外匯,為國內重要水產物種之一。吳郭魚具有許多品系,不同品系之間肌肉的特性也不盡相同,影響到魚肉的品質、加工的結果、保存的安定性等。原肌球蛋白是一種肌肉蛋白,以double α-helix構成,兩股單體形成coiled-coil雙體結構。原肌球蛋白與Ca²̽ 、actin、myosin以及troponin一同調節肌肉的收縮。原肌球蛋白是一種保守性相當高的蛋白,在目前的研究中發現,不同種類間的魚,其肌肉原肌球蛋白胺基酸序列各有少許不同的差異性,而這些不同的差異影響到TM之結構及安定性。本實驗利用國內常見的三種吳郭魚:尼羅種吳郭魚(Oreochromis nilotica)、莫三比克吳郭魚(Oreochromis mossambica)、尼羅紅魚(Oreochromis sp., Red Tilapia)為對象,取其肌肉組織以超高速離心(ultracentrifuge)、等電點沉澱(isoelectric precipitation)、硫胺分化(ammonium sulfate differentiation)等方法純化不同吳郭魚骨骼肌的原肌球蛋白,並利用DSC 及CD 探討不同吳郭魚種之原肌球蛋白結構安定性。
關鍵字:吳郭魚、原肌球蛋白(tropomyosin, TM)、結構安定性、圓二色光譜測定 (Circular Dichroism,CD)、掃描式熱差分析儀 (Differential Scanning Calorimetry, DSC)
著作名稱:Study for purification methods for crustaceous tropomyosin.
年度:2010
類別: 會議論文
摘要:Tropomyosins (TMs) form a family of actin-binding proteins that are highly conserved throughout evolution. They are found not only in muscle fibers but also in non-muscle cells. The TM is composed of twoα- subunits (approximately 33 kDa), and forms a coiled-coil structure consisting of two parallel α-helical polypeptides, which associate with troponin complex and seven actin monomers on the thin filaments of striated muscle. We are interesting in some invertebrate TMs because reference is limited and major research focus on allergen. The TMs of crustacean like shrimps and crabs are quietly different from vertebrate. To find the purified methods of crustaceous TMs is very important for next research. In this study, we used Black tiger prawn (Penaeus monodon), Giant freshwater prawn (Macrobrachium rosenbergii), Pacific white shrimp (Litopenaeus vannamei), Shiba shrimp (Metapenaeus joyneri), Mangrove crab (Scylla serrata) and Red spotted swimming crab (Portunus Sanguinolentus) as samples. We used high speed centrifugation, ammonium sulfate saturation, isoelectric point and column purification and try to find the best condition to purify the crustaceous TMs.
關鍵字:Tropomyosin, Crustacean, Protein purification.
著作名稱:刀額新對蝦(Metapenaeus ensis)原肌球蛋白(Tropomyosin)特性及熱力學研究
年度:2009
類別: 會議論文
摘要:刀額新對蝦(Metapenaeus ensis),俗稱沙蝦,廣泛分佈於台灣沿海各地,是重要的經濟蝦類之一。刀額新對蝦具有適應性強,適廣溫性,對鹽度適應範圍廣、離水後不易死亡,當冬季水溫低時,能潛入沙裏越冬故名。本研究探討刀額新對蝦肌肉TM之基礎生化特性及熱穩定性為主。研究方法為抽取蝦肉組織,利用高速離心、等電點沉澱及硫胺分化取出TM,再經由管柱層析精製TM,經由一次電泳及二次電泳分析TM特色,再將精製之TM作CD以及DSC分析。由結果得知,刀額新對蝦肌肉中以一種TM為主要存在形式。由CD可知刀額新對蝦TM大部分由α-螺旋之構成,由DSC分析可知變性溫度(Tm)約為49゚C。希望本研究資料能提供水產加工業者加深對刀額新對蝦TM之認識,並對於蛋白質結構學能有益。
關鍵字:刀額新對蝦、原肌球蛋白(tropomyosin,TM)、circular dichroism (CD)、differential scanning calorimetry (DSC)
著作名稱:李政霖、劉育涵、陳怡靜、黃銘志。濕地精靈- 彈塗魚肌肉纖維原肌球蛋白之基礎研究
年度:2009
類別: 會議論文
摘要:Mudskipper, which is also named “Spotty-bounce” or “Mud-monkey”, is common in mangrove marsh regions of Taiwan. The mudskippers commonly found in western coast of Taiwan are Periophthalmini boleophthalmus and Periophthalmus cantonesis. This mudskipper species amphibian, it can breathe with its moist skin and the moisture in the gill chamber, and adapt to life in a semiwater-landed shore. It can construct vertical underground tunnels in bayou, marsh, and mud shoal, and inhabits within. Because of the bouncing character of mudskipper and the oxygen-changing living condition, we are interested in the structure of the mudskipper`s muscle.In this study, we focus on the highly conserved muscular fibril – TM of whole muscle. We probe into the structure and the thermostability of the skeletal TM of mudskipper, and compare and analyze the data from mudskipper with other species. Firstly, we extract TM molecules using isoelectric precipitation, high speed centrifugation and ammonium sulfate saturation. Next we identify the extract of TM by SDS-PAGE and 2D-SDS-PAGE. Finally we analyze the thermostability and the 3-D structure using by differential scanning calorimetry (DSC) and circular dichroism (CD) spectrometry; respectively. The data acquired from mudskipper were compared with other fish.We found that the structure of mudskipper TM is different from other species and the thermostability is higher. Although we can not prove that the results are caused by the amphibious situation, this truely provides a new direction for subsequent studies. We hope that this research adds to the knowledge about the living styles of animal in everglade and how these animals adapt physiologically to the environments. Furthermore, the study provides insight to the significance of biological diversity and evolution.
關鍵字:彈塗魚、原肌球蛋白
著作名稱:陳怡靜、李政霖、黃銘志。(2009)台灣水產物種可能過敏原Tropomyosin差異性之研究。
年度:2009
類別: 會議論文
摘要:
關鍵字:tropomyosin
著作名稱:劉育涵、林信宏、蔡明諺、許莉苓、涂宇洲、李政霖、陳怡靜、黃銘志。(2008)尼羅吳郭魚原肌凝蛋白cDNA克隆之研究。2008台灣水產學會學術論文發表會。台灣台北 (學生壁報競賽優勝)
年度:2008
類別: 會議論文
摘要:
關鍵字:
著作名稱:台南四草濕地彈塗魚的生態環境與型態構造初探
年度:2010
類別: 會議論文
摘要:台灣西南沿海廣佈濕地地形,潮間帶泥地常有一些特殊的生物。彈塗魚是此區常見的 兩棲魚類,具有特殊適應濕地的型態及生態結構。長久以來大家都知道彈塗魚是會築巢的 ,築巢的行為除了提供彈塗魚在漲退潮時的棲息地外,在求偶行為、逃避敵害,溫度調節 等生態意義也是重要的一環。但卻鮮少有人知道彈塗魚巢穴的形狀,國內外有關彈塗魚巢 穴的文獻也相當有限。本次調查以分布於台南四草地區彈塗魚為主要探討對象,針對其巢 穴形狀作一調查,探討其生態之意義。經野外採樣調查發現,台南四草地區以廣東彈塗魚 (Periophthalmus cantonensis)為主要魚種。選定退潮時彈塗魚巢穴洞口裸露時,先趕走巢穴 內之彈塗魚,利用道具吸乾巢穴內水分,再利用固形劑注入該巢穴中,待凝固後挖出模型 ,探討彈塗魚巢穴模式及生態功能。經數個彈塗魚巢穴模型取出後發現;廣東彈塗魚的巢 穴基本樣式如Y字型,巢穴甚深可達30公分以上,巢穴之型態具有多樣性,洞口至少有兩個 以上,通常一大一小。有時一個巢穴多達四個洞口。巢穴底部通常充滿水,退潮時在洞口 乾燥狀態下底部仍有相當保水性,調查中亦發現有巢穴底部直接與河底相連通者,顯示彈 塗魚巢穴的複雜性。這些研究希望能了解更多彈塗魚適應濕地之行為生態,並能有效管理 溼地資源。
關鍵字:彈塗魚