| 著作名稱： | Dietary probiotic Aspergillus niger preparation improves the growth performance, health status, and gut microbiota of white shrimp, Penaeus vannamei. (SCI) |
| 年度： | 2023 |
| 類別： |
期刊論文
Aquaculture
|
| 摘要： | Aspergillus niger is a unique fungus with benefits that can help improve the performance of terrestrial and aquatic
animals. However, studies on the use of A. niger on aquatic animal species are limited. The present study
investigated the effects of A. niger preparation as a dietary supplement on growth, gut microbiota, and immune
response of white shrimp, Penaeus vannamei. The experiment was conducted over a 56-day trial period, with
shrimps fed a control diet, and three diets containing three doses of A. niger preparation 0.5, 1 and 1.5 g (kg
diet) 1) designated as P500, P1000 and P1500, respectively. The results showed that increasing concentrations
of A. niger preparation led to higher weight gain and final weight in shrimp. Moreover, increasing levels of
A. niger preparation of P1000 and P1500 reduced the mortality rate of shrimp challenged with Vibrio parahaemolyticus.
In A. niger groups, PA, RBs, PO and LYS activities of shrimp significantly increased compared to the
control group. Microbial analysis revealed significantly higher Vibrio-like counts in the gut of shrimp fed the
control diet than in all A. niger-supplemented diets. Shewanella belonging to the dominant phylum Proteobacteria
was highly expressed in the microbiota of shrimp fed P1000 diet. Meanwhile, Vibrio was relatively abundant in
the control group. Unique genera, such as JGI0000069P2 and Marinicella, promoted bioremediation activities,
while Halobacteriovorax aided in the elimination of V. parahaemolyticus infection in shrimp. Notably, a symbiotic
relationship between Shewanella and Halobacteriovorax bacteria in the gut jointly boosted the immune responses
of shrimp in defense against V. parahaemolyticus infection. These findings suggest that the A. niger preparation,
due to its beneficial effects, could potentially be included in the diet to promote growth and a healthy intestinal
microbiota, while also serving as a prophylactic treatment and immunological booster for shrimp. |
| 關鍵字： | Aspergillus niger, Immunological booster, Probiotic, Growth performance, White shrimp |
| 著作名稱： | The complete mitochondrial genome sequence of Onychostoma alticorpus (Cypriniformes, Cyprinidae). (SCI) |
| 年度： | 2014 |
| 類別： |
期刊論文
Mitochondrial DNA
|
| 摘要： | We determined the complete mitochondrial genome (mitogenome) sequence of Onychostoma
alticorpus, which is known as an endemic freshwater species in Taiwan, by using long
polymerase chain reaction method. The total length of O. alticorpus mitogenome is 16,680 bp,
consisting of 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a
noncoding control region. The overall base composition of O. alticorpus is 30.88% for A, 23.57%
for T, 16.56% for G and 28.99% for C, with a slight AT bias of 54.45%. Gene location and specific
usage of distinct termination codon types characterize typically the vertebrate mitochondrial
genome. The determination of O. alticorpus mitogenome would play an important role not only
in the delineation of phylogeographic history and population genetic structure, but reflection
of conservation efforts on the genetic diversity as well as population vitality. |
| 關鍵字： | Cyprinidae, mitogenome, Onychostoma alticorpus |
| 著作名稱： | Tseng DY. (2013) Complete mitochondrial genome of Onychostoma barbata (Cypriniformes, Cyprinidae). (SCI) |
| 年度： | 2013 |
| 類別： |
期刊論文
Mitochondrial DNA
|
| 摘要： | In this study, the complete mitogenome sequence of Onychostoma barbata has been determined using long polymerase chain
reaction method. The mitogenome, consisting of 16,592 base pairs (bp), had the typical vertebrate mitochondrial gene
arrangement, including 13 protein-coding, 22 transfer RNAs, 2 ribosomal RNAs genes, and a noncoding control region (CR).
CR of 937 bp lengths long is located between tRNAPro and tRNAPhe. The overall base composition of O. barbata is 24.49% for
T, 28.04% for C, 31.54% for A, and 15.94% for G, with a slight AT bias of 56.03%. |
| 關鍵字： | Onychostoma barbata, mitogenome, Cyprinidae |
| 著作名稱： | Lin C-H, Su C-H, Tseng D-Y, Ding F-C, Hwang P-P (2012) Action of Vitamin D and the Receptor, VDRa, in Calcium Handling in Zebrafish (Danio rerio). (SCI) |
| 年度： | 2012 |
| 類別： |
期刊論文
PLoS ONE
|
| 摘要： | The purpose of the present study was to use zebrafish as a model to investigate how vitamin D and its receptors interact to
control Ca2+ uptake function. Low-Ca2+ fresh water stimulated Ca2+ influx and expressions of epithelial calcium channel
(ecac), vitamin D-25-hydroxylase (cyp2r1), vitamin D receptor a (vdra), and vdrb in zebrafish. Exogenous vitamin D increased
Ca2+ influx and expressions of ecac and 25-hydroxyvitamin D3-24-hydroxylase (cyp24a1), but downregulated 1a-OHase
(cyp27b1) with no effects on other Ca2+ transporters. Morpholino oligonucleotide knockdown of VDRa, but not VDRb, was
found as a consequence of calcium uptake inhibition by knockdown of ecac, and ossification of vertebrae is impaired. Taken
together, vitamin D-VDRa signaling may stimulate Ca2+ uptake by upregulating ECaC in zebrafish, thereby clarifying the
Ca2+-handling function of only a VDR in teleosts. Zebrafish may be useful as a model to explore the function of vitamin DVDR
signaling in Ca2+ homeostasis and the related physiological processes in vertebrates. |
| 關鍵字： | vitamin D, VDR, calcium |
| 著作名稱： | Ding HY, TS Chang, CM Chiang, SY Li, DY Tseng*. 2011. Melanogenesis inhibition by a crude extract of Magnolia officinalis. (SCI) |
| 年度： | 2011 |
| 類別： |
期刊論文
Journal of Medicinal Plants Research
|
| 摘要： | In the present study, we investigated the inhibitory effect of a crude extract from Magnolia officinalis (MOE) on melanogenesis in both mouse B16 melanoma cells and zebrafish. Our results showed that MOE inhibited melanogenesis in either alpha-melanocyte stimulating hormone (alpha-MSH)- or 3-isobutyl-1-methylxanthin (IBMX)-stimulated B16 cells in a dose-dependent manner with an IC50 value of 9.3 ug/ml. In addition, MOE also inhibited cellular tyrosinase activity with an IC50 value of 13.4ug/ml while no inhibitory activity was found by MOE against cell-free tyrosinase activity. Moreover, western blotting and real time reverse-transcription polymerase chain reaction (qRT-PCR) analyses respectively confirmed that MOE downregulated levels of tyrosinase protein but not that of its mRNA inalpha-MSH-stimulated B16 cells. These results demonstrated that MOE inhibits melanogenesis of B16 cells by a post-transcriptional regulation on tyrosinase gene expression. In the other hand, when using zebrafish as a depigmenting assay system, MOE could inhibit both melanogenesis and tyrosinase activity in the in vivo model. From the present study, MOE was proven to be a good candidate as a skin-whitening agent for treatment of skin hyperpigmentation. |
| 關鍵字： | Magnolia officinalis, Melanogenesis, Tyrosinase, Melanin, Inhibition |
| 著作名稱： | Lin CH, IL Tsai, CH Su, DY Tseng, PP Hwang*. 2011. Reverse Effect of Mammalian Hypocalcemic Cortisol in Fish: Cortisol Stimulates Ca2+ Uptake via Glucocorticoid Receptor- Mediated Vitamin D3 Metabolism. (SCI) |
| 年度： | 2011 |
| 類別： |
期刊論文
PLoS ONE
|
| 摘要： | Cortisol was reported to downregulate body-fluid Ca2+ levels in mammals but was proposed to show hypercalcemic effects in teleostean fish. Fish, unlike terrestrial vertebrates, obtain Ca2+ from the environment mainly via the gills and skin rather than by dietary means, and have to regulate the Ca2+ uptake functions to cope with fluctuating Ca2+ levels in aquatic environments. Cortisol was previously found to regulate Ca2+ uptake in fish; however, the molecular mechanism behind this is largely unclear. Zebrafish were used as a model to explore this issue. Acclimation to low-Ca2+ fresh water stimulated Ca2+
influx and expression of epithelial calcium channel (ecac), 11b-hydroxylase and the glucocorticoid receptor (gr). Exogenous cortisol increased Ca2+ influx and the expressions of ecac and hydroxysteroid 11-beta dehydrogenase 2 (hsd11b2), but downregulated 11b-hydroxylase and the gr with no effects on other Ca2+ transporters or the mineralocorticoid receptor (mr).
Morpholino knockdown of the GR, but not the MR, was found to impair zebrafish Ca2+ uptake function by inhibiting the
ecac expression. To further explore the regulatory mechanism of cortisol in Ca2+ uptake, the involvement of vitamin D3 was analyzed. Cortisol stimulated expressions of vitamin D-25hydroxylase (cyp27a1), cyp27a1 like (cyp27a1l), 1a-OHase (cyp27b1) at 3 dpf through GR, the first time to demonstrate the relationship between cortisol and vitamin D3 in fish. In conclusion, cortisol stimulates ecac expression to enhance Ca2+ uptake functions, and this control pathway is suggested to be mediated by the GR. Lastly, cortisol also could mediate vitamin D3 signaling to stimulate Ca2+ uptake in zebrafish. |
| 關鍵字： | cortisol, Ca2+ uptake, glucocorticoid receptor |
| 著作名稱： | Zhao J, DY Tseng, HD Lin, XT Lin*. 2011. Isolation and characterization of microsatellite markers to study population genetic diversity of White Cloud Mountain minnow (Tanichthys albonubes Lin) in wild and cultured populations. (SCI) |
| 年度： | 2011 |
| 類別： |
期刊論文
Genetics and Molecular Research
|
| 摘要： | We developed 12 microsatellite loci for the endangered minnow species, Tanichthys albonubes, using PCR-based isolation of microsatellite arrays. These new markers were tested in 26 individuals from a wild population collected from Guangzhou in China and 26 individuals from a cultured strain. The number of alleles ranged from two to nine and the expected heterozygosity from 0.177 to 0.853. The wild population had significantly higher allelic richness than the cultured strain, with a mean allelic richness of 5.52 (range 3.69-8.64) and 3.13 (range 1.99-5.73) for the wild population and the culturedstrain, respectively. No evidence of a recent bottleneck was detected in the wild population, but it was found in the cultured strain based on the BOTTLENECK test. These primers can be used to understand the demography and to examine genetic differences between the cultured T. albonubes strains and wild populations to help determine conservation and reintroduction strategies. |
| 關鍵字： | Tanichthys albonubes; Microsatellite marker; Bottleneck; |
| 著作名稱： | Tseng DY, MY Chou, YC Tseng, CD Hsiao, CJ Huang, T Kaneko, PP Hwang. 2009. Effects of Stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology, 296: R549- 57. (SCI) |
| 年度： | 2009 |
| 類別： |
期刊論文
Am J Physiol Regulatory Integrative Comp Physiol
|
| 摘要： | Stanniocalcin (STC) formerly called hypocalcin or teleocalcin, is a 50-kDa disulfide-linked homodimeric glycoprotein that was originally identified in fish and secreted from the corpuscles of Stannius (CS). One of the main functions of STC-1 is Ca(2+) uptake inhibition; however, the mechanisms remain unknown. In the present study, we provide molecular evidence to elucidate how zebrafish STC-1 regulates Ca(2+) uptake in zebrafish embryos. In a wide variety of tissues including the kidney, brain, gill, muscle, and skin, zstc-1 was expressed. Incubating zebrafish embryos in low-Ca(2+) (0.02 mM) freshwater stimulated whole body Ca(2+) influx and zebrafish epithelial Ca(2+) channel (zECaC) mRNA expression, while downregulated zstc-1 expression. A morpholino microinjection approach was used to knockdown the zSTC-1 protein, and the results showed that the Ca(2+) content, Ca(2+) influx, and zECaC mRNA expression all increased in morphants. These data suggest that zSTC-1 negatively regulates ECaC gene expression to reduce Ca(2+) uptake in zebrafish embryos.
|
| 關鍵字： | Stanniocalcin, ECaC |
| 著作名稱： | Tseng DY, PL HO, SY Huang, SC Cheng, YL Shiu, CS Chiu, CH Liu. 2009. Enhancement of immunity and disease resistance in the white shrimp, Litopenaeus vannamei, by the probiotic, Bacillus subtilis E20. (SCI) |
| 年度： | 2009 |
| 類別： |
期刊論文
Fish & Shellfish Immunology
|
| 摘要： | Effects of Bacillus subtilis E20 isolated from fermented soybean on immune parameters and the disease resistance of the white shrimp (Litopenaeus vannamei) after 98 days of B. subtilis E20 feeding were evaluated in this study. Shrimp fed B. subtilis E20-containing diets at concentrations of 10(6) (E206), 10(7) (E207), and 10(8) (E208)cfu kg(-1), respectively, had significantly increased survival rates of 13.3%, 16.7%, and 20%, compared to the control (fed no probiotic) after being challenged with Vibrio alginolyticus. There were no significant differences in the total hemocyte count, respiratory burst, or superoxide dismutase glutathione peroxidase among all treatments. Shrimp fed a higher concentration of the probiotic (E208) exhibited significant increases in phenoloxidase activity, phagocytic activity, and clearance efficiency compared to control shrimp. In addition, B. subtilis E20 showed a weaker inhibitory effect against the growth of Aeromona hydrophila with around a 0.3-cm inhibitory zone, but showed no inhibitory effects against other selected pathogens, such as white shrimp pathogens: V. alginolyticus and Vibrio vulnificus. These results suggest that the increased resistance of shrimp after B. subtilis E20 consumption occurs through immune modifications, such as increases in phenoloxidase activity, phagocytic activity, and clearance efficiency against V. alginolyticus. |
| 關鍵字： | vannamei, subtilis |
| 著作名稱： | Hsu PI, CH Liu, DY Tseng, PP Lee, W Cheng. 2006. Molecular cloning and characterization of peroxinectin, a cell adhesion molecule, from the giant freshwater prawn, Macrobrachium rosenbergii. (SCI) |
| 年度： | 2006 |
| 類別： |
期刊論文
Fish & Shellfish Immunology
|
| 摘要： | Expression of peroxinectin cDNA was determined from haemocytes of giant freshwater prawn Macrobrachium rosenbergii using oligonucleotide primers and reverse transcription polymerase chain reaction (RT-PCR) based on the peroxinectin sequence of white shrimp Litopenaeus vannamei, tiger shrimp Penaeus monodon, and freshwater crayfish Pacifastacus leniusculus. The peroxinectin of M. rosenbergii was constitutively expressed. Analysis of the nucleotide sequence revealed that the cDNA clone has an open reading frame of 2,403 bp encoding a protein of 801 amino acids including a 20 amino acid signal peptide. The calculated molecular mass of the mature protein (781 amino acids) was 88.7 kDa with an estimated pI of 6.8. A putative peroxidase domain and a putative integrin-binding motif, KGD (Lys-Gly-Asp) were observed in prawn peroxinectin at the C-terminal. Sequence comparison showed that peroxinectin deduced amino acid of M. rosenbergii had an overall similarity of 62%, 64%, and 66% to that of P. leniusculus, P. monodon, and L. vannamei, respectively. Quantitative real-time PCR analysis showed that peroxinectin transcript in haemocyte of M. rosenbergii decreased significantly after 3, 6 and 12h injection with Lactococcus garvieae.
|
| 關鍵字： | peroxinectin, rosenbergii, prawn |
| 著作名稱： | Liu CH, DY Tseng, CY Lai, W Cheng, CM Kuo. 2006. Molecular cloning and characterization of prophenoloxidase cDNA from haemocytes of the giant freshwater prawn, Macrobrachium rosenbergii, and its transcription in relation with the moult stage. (SCI) |
| 年度： | 2006 |
| 類別： |
期刊論文
Fish & Shellfish Immunology
|
| 摘要： | Expression of prophenoloxidase (proPO) cDNA was determined from haemocytes of the giant freshwater prawn Macrobrachium rosenbergii by a reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA using oligonucleotide primers based on the proPO sequence of tiger shrimp Penaeus monodon, freshwater crayfish Pacifastacus leniusculus, green tiger shrimp Penaeus semisulcatus, kuruma shrimp Marsupenaeus japonicus, and white shrimp Litopenaeus vannamei. The proPO of M. rosenbergii was constitutively expressed. The 2,547-bp cDNA contained an open reading frame (ORF) of 2,013 bp, a 96-bp 5-untranslated region, and a 438-bp 3-untranslated region containing the poly A tail. The molecular mass of the deduced amino acid (aa) sequence (671 aa) was 76.7 kDa with an estimated pI of 7.05. It contained putative copper-binding sites, a complement-like motif (GCGWPRHM), a proteolytic activation site, and a conserved C-terminal region common to all known proPOs. However, no signal peptide sequence was detected in giant freshwater prawn proPO. Comparison of amino acid sequences showed that prawn proPO is similar to the proPO of penaeid, crayfish and lobster. Prawn proPO was only synthesised in haemocytes. The proPO transcript was significantly increased in the A stage and achieved the highest level in the B stage, and then declined sharply in the C stage and reached the lowest level in the D(2)/D(3) stage.
|
| 關鍵字： | prophenoloxidase, rosenbergii, prawn |
| 著作名稱： | Tseng DY, YN Chen, KF Liu, GH Kou, CF Lo, CM Kuo. 2002. Hepatopancreas and ovary are sites of vitellogenin synthesis as determined from partial cDNA encoding of vitellogenin in the marine shrimp, Penaeus vannamei. , (SCI) |
| 年度： | 2002 |
| 類別： |
期刊論文
Invertebrate Reproduction and Development
|
| 摘要： | The site of yolk protein synthesis in crustaceans has long been a subject of controversy. The vitellogenin gene
structure was partially reported only very recently in Macrobrachium rosenbergii, after which the hepatopancreas was
confirmed as the extraovarian site of vitellogenin synthesis in that species. Ovaries are the most frequently reported as
the site of yolk protein synthesis in penaeid shrimp. Using cDNA reversed-transcribed from mRNA isolated from the
hepatopancreas of vitellogenic female shrimp, Penaeus monodon, we found that its deduced amino acid sequence had
high identity of 48% with that from M. rosenbergii vitellogenin. A similar location of the intron in the sequenced region
of genomic DNA was also found between these two species. We therefore concluded that the hepatopancreas the
extraovarian site of vitellogenin synthesis in P. monodon in vivo. The partial structure of vitellogenin gene is presented
in this study. 2001 Elsevier Science Inc. All rights reserved. |
| 關鍵字： | vitellogenin, vannamei, shrimp |
| 著作名稱： | Tseng DY, YN Chen, GH Kou, CF Lo, CM Kuo. 2001. Hepatopancreas is the extraovarian site of vitellogenin synthesis in black tiger shrimp, Penaeus monodon. Comparative Biochemistry and Physiology, 129A: 909-917. (SCI) |
| 年度： | 2001 |
| 類別： |
期刊論文
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
|
| 摘要： | The site of yolk protein synthesis in crustaceans has long been a subject of controversy. The vitellogenin gene structure was partially reported only very recently in Macrobrachium rosenbergii, after which the hepatopancreas was confirmed as the extraovarian site of vitellogenin synthesis in that species. Ovaries are the most frequently reported as the site of yolk protein synthesis in penaeid shrimp. Using cDNA reversed-transcribed from mRNA isolated from the hepatopancreas of vitellogenic female shrimp, Penaeus monodon, we found that its deduced amino acid sequence had high identity of 48% with that from M. rosenbergii vitellogenin. A similar location of the intron in the sequenced region of genomic DNA was also found between these two species. We therefore concluded that the hepatopancreas the extraovarian site of vitellogenin synthesis in P. monodon in vivo. The partial structure of vitellogenin gene is presented in this study.
|
| 關鍵字： | vitellogenin, monodon, shrimp |
| 著作名稱： | Chen YN, DY Tseng, PY Ho, CM Kuo. 1999. Site of vitellogenin synthesis determined from a cDNA encoding a vitellogenin fragment in the freshwater giant prawn, Macrobrachium rosenbergii. , (SCI) |
| 年度： | 1999 |
| 類別： |
期刊論文
Molecular Reproduction and Development
|
| 摘要： | Vitellogenesis is an important part of reproductive process in crustaceans, and the process is characterized by the synthesis and accumulation of yolk protein in the developing oocytes. The yolk proteins in crustaceans mainly consist of vitellogenin (Vg) and vitellin (Vn), which are respectively present in extra-oocyte tissues and intra-oocytes. The site and the process of yolk protein synthesis in crustaceans are still controversial. The synthesis site of Vg in a crustacean species, Macrobrachium rosenbergii, is determined by immunological and immunohistochemical techniques, and molecular cloning of a cDNA encoding the primary structure of Vn in this study. The hepatopancrease is clearly shown to be the synthesis site of Vg in this species. The length of Vg mRNA was estimated as about 6 kb from Northern blotting analysis. The partial primary structure of Vg gene is presented, and the post-translational processing are further discussed. For the first time, the partial primary structure of Vg gene and the synthesis site of Vg approached by molecular cloning in crustaceans are presented. |
| 關鍵字： | vitellogenin, rosenbergii, prawn |
| 著作名稱： | Adenosine 5′-monophosphate-activated protein kinase (AMPK) negatively regulates the immunity and resistance to Vibrio alginolyticus of white shrimp, Penaeus vannamei (SCI) |
| 年度： | 2023 |
| 類別： |
期刊論文
Fish and Shellfish Immunology
|
| 摘要： | Shrimp immunology is vital in establishing prophylactic and therapeutic strategies for controlling pathological
problems that threaten shrimp production. Apart from dietary treatments, the adenosine 5′ -monophosphateactivated
protein kinase (AMPK), an important regulatory enzyme that restores cellular energy balance during
metabolic and physiological stress, is known to have therapeutic potential to improve shrimp’s defense mechanism.
Despite this, studies targeting the AMPK pathway in shrimp exposed to stressful conditions are vastly
limited. In this study, AMPK was knocked down to assess the immunological changes and white shrimp, Penaeus
vannamei resistance to Vibrio alginolyticus infection. Shrimps were injected individually and simultaneously with
dsRNA targeting specific genes such as AMPK, Rheb, and TOR, after which the hepatopancreas was analyzed for
the different gene expressions. The gene expressions of AMPK, Rheb, and TOR were effectively suppressed after
being treated with dsRNAs. The Western blot analysis further confirmed a reduction in the protein concentration
of AMPK and Rheb in the hepatopancreas. The suppression of AMPK gene led to a robust increase in the shrimp’s
resistance to V. alginolyticus, whereas the activation of AMPK by metformin decreased the shrimp’s disease
resistance. Among the mTOR downstream targets, the HIF-1α expression in shrimp treated with dsAMPK
significantly increased at 48 h but returned to normal levels when shrimp were treated with dsAMPK and either
dsRheb or dsTOR. Immune responses such as respiratory burst, lysozyme activity, and phagocytic activity
increased, while superoxide dismutase activity decreased following the knockdown of the AMPK gene compared
to the control group. However, co-injection with dsAMPK and dsTOR or dsRheb restored immune responses to
normal levels. Collectively, these results demonstrate that the inactivation of AMPK may ameliorate shrimp’s
innate immune response to recognize and defend against pathogens via the AMPK/mTOR1 pathway. |
| 關鍵字： | Shrimp AMPK/mTOR1 pathway Gene knockdown Innate immune defense |
| 著作名稱： | Dietary administration of a postbiotic, heat-killed Pediococcus pentosaceus PP4012 enhances growth performance, immune response and modulates intestinal microbiota of white shrimp, Penaeus vannamei (SCI) |
| 年度： | 2023 |
| 類別： |
期刊論文
Fish and Shellfish Immunology
|
| 摘要： | The efficacy of postbiotics on the immune-related gene expression and gut microbiota of white shrimp, Penaeus
vannamei remains unexplored. A commercial heat-killed postbiotic Pediococcus pentosaceus PP4012 was used to
evaluate the growth performance, intestinal morphology, immunological status, and microbial community of
white shrimp after dietary administration in this study. White shrimp (0.040 ± 0.003 g) were divided into three
treatments; a control, inanimate P. pentosaceus (105 CFU g feed 1) at low concentration (IPL) and inanimate
P. pentosaceus (106 CFU g feed 1) at high concentrations (IPH). The diets of IPL and IPH significantly increased
final weight, specific growth rate and production compared to the control group. Shrimp fed with IPL and IPH
significantly utilized feed more efficiently than those fed the control diet. The IPH treatment significantly
lowered the cumulative mortality rate compared to the control and IPL diet following Vibrio parahaemolyticus
infection. No significant difference was observed for Vibrio-like and lactic acid bacteria in intestine of shrimp fed
with the control diet and the experimental diets. Adding inanimate P. pentosaceus significantly improved immune
responses such as lysozyme and phagocytic activity compared to the control group. However, the total hemocyte
count, phenoloxidase activity, respiratory burst, and superoxide dismutase activity were not significantly
different among treatments. The immune-related genes alf, pen3a, and pen4 expression were significantly higher
in shrimp fed IPL diet compared with control and IPH. Taxonomic identification of bacterial genera in all dietary
groups belonged to two predominant phyla, Proteobacteria and Bacteroidota. An abundance of Photobacterium,
Motilimonas, Litorilituus, and Firmicutes bacterium ZOR0006 were identified in the intestine of shrimp fed postbiotic
diets. Unique microbes such as Cohaesibacter was discovered in the shrimp fed IPL while Candidatus
Campbellbacteria, uncultured Verrucomicrobium DEV114 and Paenalcaligenes were discovered in the intestines of
shrimp fed IPH diet. Collectively, these data suggest that including heat-killed P. pentosaceus, particularly IPH,
can enhance growth performance, promote microbial diversity, elevate immune responses, and increase shrimp’s
resistance to V. parahaemolyticus. |
| 關鍵字： | Postbiotic Pediococcus pentosaceus Growth performance Immunological status Intestinal microbiota |
| 著作名稱： | Glucocorticoid Receptor Mediates Cortisol Regulation of Glycogen Metabolism in Gills of the Euryhaline Tilapia (Oreochromis mossambicus) (SCI) |
| 年度： | 2023 |
| 類別： |
期刊論文
FISHES
|
| 摘要： | In this study, we investigated the effects of cortisol on the regulation of the glycogen
metabolism biomarkers glycogen synthase (GS) and glycogen phosphorylase (GP) in the glycogenrich
cells of the gills of tilapia (Oreochromis mossambicus). In the gills of tilapia, GP, GS, and glycogen
were immunocytochemically colocalized in a specific group of glycogen-rich cells adjacent to the
gills’ main ionocytes and mitochondria-rich cells. Cortisol plays a vital role in the regulation of
physiological functions in animals, including energy metabolism, respiration, immune response,
and ion regulation. However, no studies have elucidated the mechanisms regulating cortisol and
glycogen-rich cells in the gills. Therefore, we treated tilapia larvae with exogenous cortisol and a
glucocorticoid receptor (GR) antagonist to investigate the regulatory mechanisms between cortisol
and glycogen-rich cells in the gills. Our results showed that cortisol promoted the expression of gill
glycogen phosphorylase isoform (GPGG) mRNA via GR, whereas the GS gene expression remained
unaffected. We also found that GR mRNA was colocalized with some glycogen-rich cells in the
gills, further confirming our hypothesis that cortisol directly acts on glycogen-rich cells in the gills of
tilapia and regulates glycogen metabolism by promoting GPGG mRNA expression. |
| 關鍵字： | cortisol; glycogen-rich cell; glycogen phosphorylase; glycogen synthase; ionocytes; glucocorticoid receptor; tilapia |
| 著作名稱： | Mineralocorticoid Receptor Mediates Cortisol Regulation of Ionocyte Development in Tilapia (Oreochromis mossambicus) (SCI) |
| 年度： | 2023 |
| 類別： |
期刊論文
FISHES
|
| 摘要： | Cortisol is the predominant corticosteroid in ray-finned fish since it does not possess
the aldosterone synthase necessary to produce specific mineralocorticoids. Cortisol is traditionally
believed to function as a fish mineralocorticoid. However, the effects of cortisol are mediated
through corticosteroid receptors in other vertebrates, and there is an ongoing debate about whether
cortisol acts through the glucocorticoid receptor (GR) or the mineralocorticoid receptor (MR) in
teleosts. To investigate this issue, we conducted a study using euryhaline Mozambique tilapia
(Oreochromis mossambicus) as the experimental species. The experiment was designed to investigate
the effect of cortisol on ionocyte development at both the cellular and gene expression levels in tilapia.
We administered exogenous cortisol and receptor antagonists, used immunohistochemistry to quantify
ionocyte numbers, and performed real-time PCR to assess the expression of the differentiation
factor tumor protein 63 (P63) mRNA, an epidermal stem cell marker. We observed that cortisol
increased the number of Na+-K+-ATPase (NKA)-immunoactive ionocytes (increased by 1.6-fold)
and promoted the gene expression of P63 mRNA (increased by 1.4-fold). Furthermore, we found
that the addition of the mineralocorticoid receptor antagonist Spironolactone inhibited the increase
in the number of ionocytes (decreased to the level of the control group) and suppressed the gene
expression of P63 (similarly decreased to the level of the control group). We also provided evidence
for gr, mr, and p63 localization in epidermal cells. At the transcript level, mr mRNA is ubiquitously
expressed in gill sections and present in epidermal stem cells (cells labeled with p63), supporting
the antagonism and functional assay results in larvae. Our results confirmed that cortisol stimulates
ionocyte differentiation in tilapia through the MR, rather than the GR. Therefore, we provide a new
direction for investigating the dual action of osmotic regulation and skin/gill epithelial development
in tilapia, which could help resolve previously inconsistent and conflicting findings. |
| 關鍵字： | cortisol; ionocytes; ionocyte development; mineralocorticoid receptor; P63; tilapia |
| 著作名稱： | Chitin derived from Daphnia similis and its derivate, chitosan, promote growth performance of Penaeus vannamei. (SCI) |
| 年度： | 2021 |
| 類別： |
期刊論文
Aquaculture
|
| 摘要： | Chitin isolated from Daphnia similis and its derivate, chitosan, were prepared to evaluate the effects on the
improvement of growth performance of white shrimp, Penaeus vannamei. The diets containing 0.4% chitin or
0.4% chitosan were fed to shrimp for 56 days to evaluate the growth performance of shrimp. A diet without
chitin or chitosan supplementation was used as the control diet (herein known as CD). No significant difference
in survival of shrimp among groups was observed. The Shrimp fed a diet containing 0.4% chitosan significantly
had much higher final weight, specific growth rate and percent weight gain as well as increased its protease
activity in hepatopancreas. The feed conversion ratio was also increased in the 0.4% chitosan-fed shrimp. In
addition, higher gene expressions of the chitinase (CHT) 3 and chitin synthase (CHS) in hepatopancreas, and the
CHT6 and CHS in cuticle were also recorded in chitosan-treated shrimp as compared to CD-treated shrimp.
Chitosan derived from chitin of D. similis is considered to have great potential to promote the shrimp growth, by
increasing the digestive enzyme and the gene expressions of CHTs and CHSs. |
| 關鍵字： | Daphnia similis, Chitin, Chitosan, White shrimp, Growth performance |
| 著作名稱： | Environmental and cortisol‑mediated control of Ca2+ uptake in tilapia (Oreochromis mossambicus). (SCI) |
| 年度： | 2016 |
| 類別： |
期刊論文
Journal of Comparative Physiology B
|
| 摘要： | Ca2+ is a vital element for many physiological
processes in vertebrates, including teleosts, which live
in aquatic environments and acquire Ca2+ from their surroundings.
Ionocytes within the adult gills or larval skin
are critical sites for transcellular Ca2+ uptake in teleosts.
The ionocytes of zebrafish were found to contain transcellular
Ca2+ transporters, epithelial Ca2+ channel (ECaC),
plasma membrane Ca2+-ATPase 2 (PMCA2), and Na+/
Ca2+ exchanger 1b (NCX1b), providing information about
the molecular mechanism of transcellular Ca2+ transports
mediated by ionocytes in fish. However, more evidence is
required to establish whether or not a similar mechanism of
transcellular Ca2+ transport also exists in others teleosts. In
the present study, ecac, pmca2, and ncx1 were found to be
expressed in the branchial ionocytes of tilapia, thereby providing
further support for the mechanism of transcellular
Ca2+ transport through ionocytes previously proposed for
zebrafish. In addition, we also reveal that low Ca2+ water
treatment of tilapia stimulates Ca2+ uptake and expression
of ecac and cyp11b (the latter encodes a cortisol-synthesis
enzyme). Treatment of tilapia with exogenous cortisol(20 mg/l) enhanced both Ca2+ influx and ecac expression.
Therefore, increased cyp11b expression is suggested
to enhance Ca2+ uptake capacity in tilapia exposed to low
Ca2+ water. Furthermore, the application of cortisol receptor
antagonists revealed that cortisol may regulate Ca2+
uptake through glucocorticoid and/or mineralocorticoid
receptor (GR and/or MR) in tilapia. Taken together, the data
suggest that cortisol may activate GR and/or MR to execute
its hypercalcemic action by stimulating ecac expression in
tilapia. |
| 關鍵字： | ECaC · Ca2+ influx · Ionocyte · Cortisol · Tilapia |
| 著作名稱： | Tseng DY*, SY Li. 2011. Hydroxybenzyl Alcohols Inhibit Melanogenesis in Mouse B16 Melanoma Cells. 國立台南大學環境與生態學報, 4(1):55-64 |
| 年度： | 2011 |
| 類別： |
期刊論文
環境生態學報
|
| 摘要： | Previous studies have shown that 4-hydroxybenzyl alcohols (4HBA) inhibited tyrosinase activity with an irreversible-like mode. In the present study, the inhibitory mechanism of 4HBA on tyrosinase activity was evaluated and the depigmenting activity of HBA derivatives was determined in both mouse B16 melanoma cells. It was demonstrated that 4HBA is not an irreversible inhibitor of tyrosinase. Among the HBA derivatives, 2HBA had the most potent inhibitory activity against melanogenesis in B16 cells. In addition, we also showed that 2HBA reduced melanogenesis of B16 cells by directing inhibiting tyrosinase activity. |
| 關鍵字： | Hydroxybenzyl alcohols; inhibition; melanogenesis; tyrosinase |
| 著作名稱： | Role of Stanniocalcin-1 in regulation of the expression and function of zebrafish epithelial calcium channels |
| 年度： | 2008 |
| 類別： |
會議論文
|
| 摘要： | |
| 關鍵字： | |
| 著作名稱： | Molecular characterization and expression of a novel vitellogenin gene in the freshwater giant prawn, Macrobrachium rosenbergii. |
| 年度： | 2007 |
| 類別： |
會議論文
|
| 摘要： | |
| 關鍵字： | |
| 著作名稱： | 皮質醇影響廣鹽性吳郭魚表皮離子細胞之分化與生理功能 |
| 年度： | 2019 |
| 類別： |
會議論文
|
| 摘要： | Cortisol is the main corticosteroid in teleost fishes and has long been considered to be involved in the regulation of physiological function and differentiation of epidermal ionocyteof fish, but whether it acts through the glucocorticoid receptor (GR) or the mineralocorticoid receptor (MR) remains a subject of debate. In this study,we used corticosteroid receptor antagonists to determine whether cortisol affects tilapia epidermal ionocytedevelopment and function via the GR and/or the MR. We report that that treatment with MR antagonist dramatically decreases the stimulatory effect of exogenous cortisol on the density of NKA type ionocytesand p63 mRNA expression. Cortisol significantly stimulated glycogen phosphorylase (GP) mRNA expression. On the other hand, weprovide evidence for GR and MR localization in epidermal cells. Altogether, we surmise that the effects of cortisol on differentiation and physiological function of epidermal ionocytein euryhalinetilapia are different from freshwater fish. Cortisol regulates energy metabolism on epidermal ionocyteto response in short-term salinity changes via GR. For long-term osmotic homeostasis, cortisol promotes differentiation of epidermal ionocytevia MR. |
| 關鍵字： | |
| 著作名稱： | 皮質醇透過鹽皮質類固醇受體調控廣鹽性吳郭魚魚鰓 上皮分化因子P63 之表現 |
| 年度： | 2018 |
| 類別： |
會議論文
|
| 摘要： | 皮質醇長期被認為參與魚鰓的滲透壓調節，是調控魚類適應海水的重要內分泌激素。過去研究已經證實上皮分化因子P63參與硬骨魚離子細胞早期的發育與細胞分化。利用原位雜交反應偵測mRNA 的表現位置，初步發現，鹽皮質類固醇受體（MR） mRNA表現位置與上皮分化因子P63 mRNA相鄰，表示表皮幹細胞可能受到鹽皮質類固醇受體（MR）之訊息調控。藉由添加皮質醇以及兩種類固醇受體拮抗劑實驗，皮質醇促進上皮分化因子P63之基因表現，拮抗鹽皮質類固醇受體（MR）後上皮分化因子P63之基因表現顯著下降。結果表明皮質醇透過鹽皮質類固醇受體（MR）調節上皮分化因子P63之基因表現。 |
| 關鍵字： | 吳郭魚、皮質醇、鹽皮質類固醇受體、上皮分化因子P63 |
| 著作名稱： | 皮質醇經由富含肝醣細胞調控廣鹽性吳郭魚魚鰓離子調節 |
| 年度： | 2017 |
| 類別： |
會議論文
|
| 摘要： | Cortisol has long been considered to be involved primarily in fish gill osmoregulation during acclimation to seawater. Scientific research and other studies have demonstrated the role that cortisol regulated calcium uptake in tilapia. We detected the location of glucocorticoid receptor (GR) mRNA via In situ hybridization. It was found that the location of glucocorticoid receptor (GR) mRNA beside gill ionocytes. These cells may be to provide energy as the glycogen-rich cells or epidermal stem cells. We found that glucocorticoid Receptor（GR） was colocalized with glycogen synthase（GS） in the glycogen-rich cells, not with Na+K+-ATPase in gill ionocytes. Cortisol affected the gene expression of glycogen synthase (GS) and glycogen phosphatase (GP). Cortisol increased glycogen phosphatase (GP) mRNA expression and decreased glycogen synthase (GS) mRNA expression in tilapia. |
| 關鍵字： | cortisol, glucocorticoid receptor, glycogen-rich cells |
| 著作名稱： | 皮質醇調控廣鹽性吳郭魚 (Oreochromis mossambicus)鈣離子調節之機制 |
| 年度： | 2015 |
| 類別： |
會議論文
|
| 摘要： | |
| 關鍵字： | |
| 著作名稱： | 皮質醇調節吳郭魚鈣離子吸收作用 |
| 年度： | 2011 |
| 類別： |
會議論文
|
| 摘要： | |
| 關鍵字： | |
| 著作名稱： | 中草藥抗神經壞死症病毒物質之研究 |
| 年度： | 2010 |
| 類別： |
會議論文
|
| 摘要： | |
| 關鍵字： | |
| 著作名稱： | 利用斑馬魚篩選抗紫外線曬傷之小分子天然物 |
| 年度： | 2009 |
| 類別： |
會議論文
|
| 摘要： | |
| 關鍵字： | |