國立臺南大學教師基本資料

基本資料
姓名 黃銘志
系所 生物科技學系
職稱 副教授
校內分機 7730
傳真 06-2606153
辦公室/研究室 06-260-6123轉7730
E-mail mingchih39@mail.nutn.edu.tw
網址
專長/研究領域 系統生物學 水產工學
 

畢業學校國別主修學門學位修業期間
東京大學日本水圈生物工學博士2001/4-2004/3

服務機關部門 / 系所職稱服務期間
宜蘭縣政府農業局漁業課技士1998/12-1999/3
台灣省政府農林廳漁業局養殖科技佐1999/4-1999/6
行政院農業委員會漁業署養殖沿近海漁業科技佐1999/7-2001/2
行政院農業委員會漁業署養殖沿近海漁業科技士2001/3-2004/6
日本理化學研究所基因總合研究中心準研究員2004/7~2008/3
日本理化學研究所基幹研究所準研究員2008/4~2008/8
國立台南大學生物科技學系助理教授2008/8-2014/2
國立台南大學生物科技學系副教授2014/3-

著作
名稱M.C. Huang and Y. Ochiai
年度2015
類別專書
摘要本文為敘述魚類元肌球蛋白結構及熱穩定性之文章,敘述元碁球蛋白研究之歷史及今後發展之方向。
關鍵字fish, tropomyosin, evolution.
名稱水から出た魚たち―ムツゴロウとトビハゼの挑戦 (ISBN:978-4905930174)
年度2015
類別專書
摘要
關鍵字
名稱海洋文化國際學術研討會會後論文集. 2016.
年度2016
類別專書
摘要
關鍵字
名稱Chen-Huei Huang, Ming-Chih Huang and An-Chin Lee.(1998)Characteristics of lipid peroxidation in sarcoplasmic reticulum of Tilapia. Food Sci. 25:104-108.
年度1998
類別期刊論文
摘要 
關鍵字 
名稱Chen-Huei Huang, Ming-Chih Huang and Ping-Chun Hou. (1998) Effect of dietary lipids on fatty acid composition and lipid peroxidation in sarcoplasmic reticulum of hybrid tilapia, Oreochromis niloticus × O. aureus. Comp. Biochem. Physiol. 120B:331-336.
年度1998
類別期刊論文
摘要 
關鍵字 
名稱Yoshihira Ochiai, Ming-Chih Huang, Hideto Fukushima, and Shugo Watabe. (2003) cDNA cloning and thermodynamic properties of tropomyosin from walleye pollack Theragra chalcogramma fast skeletal muscle. Fish. Sci. 69:1031-1036.
年度2003
類別期刊論文
摘要 
關鍵字 
名稱Ming-Chih Huang, Yoshihira Ochiai, and Shugo Watabe. (2004)Characterization of tropomyosin from fast skeletal muscle of bluefin tuna Thunnus thynnus. Fish. Sci. 70:667-674. (SCI)
年度2004
類別期刊論文
摘要 
關鍵字 
名稱Ming-Chih Huang, Yoshihira Ochiai.(2005)Fish fast skeletal muscle tropomyosins show species-specific thermal stability. Comp Biochem Physiol B Biochem Mol Biol. 141:461-471. (SCI)
年度2005
類別期刊論文
摘要 
關鍵字 
名稱Ming-Chih Huang, Motohiro Okada, Fubito Nakatsu, Hirokazu Oguni, Masatoshi Ito, Kohtaro Morita, Hiroshi Nagafuji, Shinichi Hirose, Sunao Kaneko, Hiroshi Ohno, Yoshiyuki Sakaki and Toshio Kojima. (2007) Mutation Screening of AP3M2 in Japanese Epilepsy Patients. Brain Dev. 29: 462-467. (SCI)
年度2007
類別期刊論文
摘要 
關鍵字 
名稱Gene network analysis to determine the effects of antioxidant treatment in a rat model of neonatal hypoxic-ischemic encephalopathy
年度2011
類別期刊論文
摘要
關鍵字
名稱Thermodynamic characterization of skeletal muscle tropomyosin from mudskipper (Boleophthalmus pectinirostris)
年度2011
類別期刊論文
摘要Mudskipper (Periophthalmini boleophthalmus) is widely distributed in coastal areas and wetland in Taiwan. The fish has a well adaptability for changes to the aquatic environment like salinity, dissolved oxygen and temperature. The purpose of this study is to investigate the biochemical characteristics and thermal stability of muscle tropomyosin (TM) from mudskipper. We extracted the crude TM from mudskipper skeletal muscle by high speed centrifugation, isoelectric point, and salting out TM with ammonium sulfate. Then we purified TM by column chromatography and detected its characteristics by electrophoresis gel analysis, circular dichroism (CD), and differential scanning calorimetry (DSC). The results show the muscle TM from mudskipper skeletal muscle has only one major form of TM. The DSC analysis showed Tm near 44 ℃. The CD spectra know the major structure of TM protein is α-helix. We wish this study can provide some data for biodiversity and evolution in mudskipper, and can be useful for protein structure study.
關鍵字mudskipper , tropomyosin (TM), circular dichroism (CD), differential scanning calorimetry (DSC)
名稱鮪類異常肉發生之原因
年度2011
類別期刊論文
摘要
關鍵字鮪魚 ; 肉質 ; 異常肉
名稱鮪類異常肉發生之原因
年度2011
類別期刊論文
摘要
關鍵字
名稱Mudskippers in Tainan: The ecology and lifestyleof mudskipper Periophthalmus modestus in Tainan coastal wetland.
年度2013
類別期刊論文
摘要The mudskipper is one of the important wetland species. It plays a key role in the conservation of wetlands and waterfowl. The special and changing environment has given rise to the evolution of many physiological mechanisms adapted to wetlands. With high sensitivity to environmental changes, mudskippers are considered as indicator organisms of wetland environmental changes. This survey is focused on the ecology and lifestyle of the mudskippers in Tainan wetland, including mudskipper species, population size, Body Mass Index change, environmental change, ecological behavior, courtship and reproduction, nest analysis, etc. The results show that there are at least two species; namely, Periophthalmus modestus and Boleophthalmus pectinirostris in the coastal wetlands of Tainan with the population size growing and declining dynamically along with the natural environment and human activity. Moreover, there are growth changes affected by the environment. Based on three observation points; namely, Keliao, Mangrove and Anping, mudskippers grow best in Mangrove while thrive poorly in Anping. Salinity is the main environmental factor while dissolved oxygen is the second. The nest is found to have a dendritic structure. The mudskippers environmental tolerance is not strong as expected. This may cause mudskippers to disappear due to their vulnerability to environmental changes such as embankments, fish farms, roads and other public buildings that bring cement and affect their habitat. Relevant authorities are expected to include mudskippers in the list of endangered species and begin conservation measures that give importance to mudskipper ecology and related wetland research that will help develop a sustainable society and country.
關鍵字Mudskipper
名稱The complete mitochondrial genome of the gnomefish Scombrops boops (Teleostei, Perciformes, Scombropidae) from the Pacific Ocean off the Japanese Islands. (SCI, IP:1.701, R128/165, Genetic & Heredity)
年度2014
類別期刊論文
摘要The complete mitochondrial genome of the gnomefish Scombrops boops was determined by a PCR-based method. The total length of mitochondrial DNA (mtDNA) was 16,517 bp, including 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes and one control region. The mitochondrial gene arrangement of the gnomefish mtDNA was identical to those of typical teleosts. This is the first report of the complete mitochondrial genome of a member of the Scombropidae family and will be useful for the development of molecular tools for ecological research.
關鍵字Fish, gnomefish, mtDNA, Scombropid fish
名稱The biochemical property between “Sik-Bah” and burnt meat from Pacific Bluefin tuna (Thunnus oriental)
年度2015
類別期刊論文
摘要 From April to July, the Pacific bluefin tuna (Thunnus thynnus orientalis) will migrate to Taiwans east coast every year. The bluefin tuna is one of the important fishery species in Taiwan. Since bluefin tuna taste good, it often as sashimi and other Japanese cuisine advanced ingredients. In those years, the reduced amount of resources to make bluefin tuna renew high prices records in the international market. It is important for meat colors of bluefin tunas, red meat is fresh and quality assurance. But the Donggang fishermen legend meat that presents a pale, soft and a little sour of tuna meat known as "cooked-meat" (pronounced Taiwanese to Sik-Bah). Cooked-meat is an abnormal meat, often occurs in the case of tuna muscle, especially bluefin tuna (Thunnus thynnus), bigeye tuna (Thunnus obesus) and yellowfin tuna (Thunnus albacares). Donggang fishermen describe the main features of cooked-meat as follow; pale flesh, lack of flexibility and sour. Foreign reports "burnt meat" are very similar to cooked-meat because their features also have pale, lack of flexibility, slightly sour and others. Is the "cooked-meat" same as "burnt meat"? Because Taiwan have no any references, it is difficult to determine. This paper aims to explore the biochemical property between "burnt meat" and "cooked-meat". In order to know the characteristics of cooked-meat, interviews for tuna related worker 30, and sampling cooked meat for laboratory analysis including pH, color quality (L*, a*, b*) and electrophoresis. The results of interview by Donggang fishermen show that the property of cooked-meat including pale (90%), soft (73%), sour (67%), rancid (53%). May cause cooked meat at a temperature factor was the highest (93%), followed by storage mode (53%), the period time of death (37%), sun or moon irradiation (30%), gender impact (30%), species strain (27%), death struggle (20%). The results of the data showed that the pH value of cooked-meat (5.56 ± 0.1, n14) was lower than normal meat (5.96 ± 0.2, n14). The color values of brightness (L*) (39.61 ± 3.36 vs 29.07 ± 2.19, n14), blue and yellow degree (b*) (12.41 ± 2.88 vs 7.77 ± 2.90, n14) in cooked-meat significantly higher than normal one. The SDS-PAGE electrophoresis analysis for cooked-meat from Donggang collected shown near 50 kDa protein band degradation. To be compared with papers reported from Yamashita and Ochiai, the degraded protein was creatine kinase. From the above results, the conclusion that "cooked-meat" was the same as "burnt meat." In this study, we make sure that "cooked-meat" was "burnt meat." We will design new study by burnt meat references.
關鍵字黑鮪、熟肉
名稱Structural and Thermodynamic Properties of Tropomyosins from the Ordinary and Hybrid Tilapia
年度2016
類別期刊論文
摘要Tilapia is a very common aquaculture species in Taiwan and both the so-called freshwater tilapia (Oreochromis niloticus) and seawater tilapia (O. mossambica) are available. In order to characterize muscle tropomyosin from these tilapia and hybrid ones (O. mossambica × O. niloticus ), the biochemical properties of tropomyosin among these tilapia species were analysed. The nucleotide sequences of tropomyosin genes were determined by cDNA cloning, and the amino acid sequences were deduced. Fast skeletal muscle tropomyosins were isolated by the combination of isoelectric point precipitation and salting out with ammonium sulfate. The thermodynamic properties of the purified tropomyosins were examined by differential scanning calorimetry (DSC) and circular dichroism (CD) spectrometry. The results showed that those tropomyosins were identical to each other including the deduced amino acid sequence, thermodynamic patterns as measured by DSC and CD, except the partial differences in the nucleotide sequence, namely, at 99th (A/G), 183rd (T/C) and 846th (T/C) positions in the open reading frame.
關鍵字Tilapia; Hybrid; Tropomyosin; cDNA cloning; Fast skeletal muscle; Thermal stability
名稱The tropomyosin structure and phylogenetic analysis of five fish species
年度2016
類別期刊論文
摘要 Fish are vertebrates and the largest number of biological species, they also have high degree of biodiversity. In order to survival in different environments, fish develop many patterns or mechanisms to adapt surrounding changes. For this reason, there are many fishes look similar but not the same species. To identify a correct species is an difficult but important issue for fish science. Today species identification and classification of commonly used molecular markers including mitochondrial cytochrome b and cytochrome c oxidase subunit I. But not all of them are applicable for above systems. This study wanted to use tropomyosin as a new molecular mark ers for fish identification, classification and development. Movement proteins are long-standing presence protein, one of movement proteins, tropomyosin can coordination of regulation and the promotion of muscle contraction with actin and myosin. This study use highly conserved features of tropomyosins as a molecul ar markers by DNA or protein sequences. The use of the logged database of fish muscle tropomyosin amino acid and DNA sequences to calculate and simulation five fish relationship including white croaker (Pennahia argentata ), northern bluefin hula (Thunnus thynnu s), tiger pufferfish (Tnktfugu rnbr ipes), zebrafish (Donio rerio), Atlantic salmon (Salmo salar). We will investigate whether tropomyosin can become a good molecular markers or not. By results, we found the primary and second structure of tropomyosin were different among the five fishes. The various of structure could help the fish with some different biological functions to adapt environment. The results of used tropomyosin as molecular marker to establish phylogenetic tree were more close to traditional theory than used other marker s. This means that tropomyosin can be used as molecular markers for fish evolution and phylogenetic analysis . The study wish to take views to explain the relationship between biological evolution by tropomyosin and a new molecular marker to species identification.
關鍵字fish、muscle、tropomyosin、cytochrome b、cytochrome c oxidase subunit I
名稱Sexual maturation of Girella punctata and G. leonina (Perciformes: Girellidae) in the neritic sea off the Pacific coast of Japan
年度2017
類別期刊論文
摘要The spawning ecology of the girellid fishes in Japanese waters has been reported almost only for Girella punctata, whereas little is known about Girella leonina. We examined sexual maturation of G. punctata and G. leonina collected from the coastal waters (neritic) of the Izu Peninsula and Tanegashima Island, based on gonadosomatic index (GSI) and gametogenesis, in order to clarify interspecific differences in spawning ecology. The GSI values of G. punctata in the Tanegashima Island region were markedly high in March, a month prior to the peak in April in the Izu Peninsula region. Girella punctata with elevated GSI had histologically mature ovaries and testes in spring in the both regions, suggesting spawning at this time of year. In contrast, the GSI values of G. leonina were much lower in the both regions, but the maximum GSI of both male and female G. leonina were slightly higher in the Tanegashima Island region than in the Izu Peninsula region. Furthermore, oogenesis in G. leonina was more advanced in the Tanegashima Island region. It is thus probable that Tanegashima Island is relatively near the spawning ground of G. leonina.
關鍵字Girella punctata, Girella leonina
名稱宜蘭產正櫻蝦梭形附頭虱(Holophryxus fusiformis)的發現與鑑定
年度2017
類別期刊論文
摘要
關鍵字正櫻蝦梭形附頭虱 ; 櫻花蝦 ; 寄生蟲
名稱Evaluation of Biochemical Properties of Burnt and Normal Meat in Pacific Bluefin Tuna (Thunnus orientalis)
年度2017
類別期刊論文
摘要Abstract: Burnt meat is one of the unusual meats often occurring in tuna species. In order to reveal the biochemical properties of burnt meat in tuna, burnt meat and normal meat samples were collected to examine the quality parameter including pH, color parameters, lactic acid content, the activities of antioxidant enzymes, and the levels of thiobarbituric acid reactive substances (TBARS) and scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. The results showed pH was significantly lower in burnt meat than in normal meat, and the lactic acid content was generally higher in burnt meat. In color parameters, the L* and b* values were higher in burnt meat. Besides, the activities of antioxidant enzymes, namely, superoxide dismutase (SOD),glutathione reductase (GR) and catalase (CAT) in normal meat were higher than those in burnt meat. TBARS levels were significantly higher in burnt meat than in normal meat. It is thus likely that strong struggle of fish throughout catch and high water temperature induce partial inactivation of these antioxidant enzymes, resulting in free radical formation which triggers the occurrence of burnt meat. This fact could be beneficial for aquaculture and livestock industry of tunas to prevent burnt meat.
關鍵字Burnt Meat, Bluefin Tuna, Antioxidant Activity, Lipid Oxidation
名稱Availability of Myoglobin as a Molecular Marker for Phylogenetic Relationships of Fish
年度2018
類別期刊論文
摘要In order to elucidate the phylogenetic relationship of fish, DNA and deduced amino acid sequences of myoglobin (Mb) were used for the phylogenetic analyses based on different approaches, namely, maximum likelihood (ML), neighbor joining (NJ), unweighted pair group method with arithmetic means (UPGMA) and maximum parsimony (MP) methods in comparison with the conventional molecular markers, mitochondrial cytochrome b (cyt-b) and cytochrome c oxidase subunit I (COI). The phylogenetic trees drawn based on Mb sequences were similar to those by the traditional classification based on the other molecular markers. The primary and secondary structures, as well as the modeled tertiary structures of Mbs were similar to each other, but were clearly distinguishable among those species. Such differences in structure would be associated with adaptation of Mb molecule to the physiological conditions of each species. These results suggest that Mb can be a molecular marker for the phylogenetic relationship of fish.
關鍵字Myoglobin, mitochondrial cytochrome b (cyt-b), cytochrome c oxidase subunit I (COI), phylogeny.
名稱Ming-Chih Huang, Motohiro Okada, Fubito Nakatsu, Hirokazu Oguni, Masatoshi Ito, Kohtaro Morita, Hiroshi Nagafuji, Shinichi Hirose, Sunao Kaneko, Hiroshi Ohno, Yoshiyuki Sakaki and Toshio Kojima.  Mutation Screening of AP3M2 in Japanese Epilepsy Patients. Human Genome Organization (HUGO) Human Genome Meeting (HGM2007) in Montreal, May 20-24, 2007. Canada
年度2007
類別研討會
摘要 
關鍵字 
名稱劉育涵、林信宏、蔡明諺、許莉苓、涂宇洲、李政霖、陳怡靜、黃銘志。(2008)尼羅吳郭魚原肌凝蛋白cDNA克隆之研究。2008台灣水產學會學術論文發表會。台灣台北 (學生壁報競賽優勝)
年度2008
類別研討會
摘要 
關鍵字 
名稱陳怡靜、李政霖、黃銘志。(2009)台灣水產物種可能過敏原Tropomyosin差異性之研究。陽明山華岡2009年生物多樣性及環境生態研討會。台灣台北
年度2009
類別研討會
摘要
關鍵字tropomyosin
名稱Ming-Chih Huang.(2009) The study of tropomyosins from tropical fish muscle. "Seminar Invitation for Laboratories of Marine Biochemistry, the University of Tokyo" July 11-13, 2009 in Chiba, Japan.
年度2009
類別研討會
摘要 
關鍵字 
名稱Toshio Kojima, Yuto Ueda, Aya Kitamoto, Akira Sato, Naoki Adati, Ming-Chih Huang. (2009) Gene expression profiling of a rat model of neonatal hypoxic-ischemic encepharopathy using DNA microarray. The Japan Neuroscience Society. September 16- 18, Nagoya, Japan
年度2009
類別研討會
摘要 
關鍵字 
名稱Ming-Chih Huang, Shuichi Yoshida, Motohiro Okada, Kohtaro Morita, Hiroshi Nagafuji, Shinichi Hirose, Sunao Kaneko, and Toshio Kojima. (2009) Mutation screening of GABRG2 gene in some types of epilepsy patients. The 43rd Congress of the Japan epilepsy society, international symposium. October 24, 2009, Hirosaki, Japan.
年度2009
類別研討會
摘要 
關鍵字 
名稱論清代曹廷杰《西伯利亞東偏紀要》中的麻特勒魚
年度2016
類別研討會
摘要
關鍵字
名稱《詩經》中的「鱣」、「鱏」、「鮪」物種考證
年度2016
類別研討會
摘要
關鍵字
名稱台灣太平洋黑鮪熟肉分析及探討
年度2016
類別研討會
摘要
關鍵字
名稱台湾近海産のクロマグロで発生したヤケ肉の生化学特徴につきまして
年度2016
類別研討會
摘要
關鍵字
名稱《遼史》中的牛魚考證
年度2016
類別研討會
摘要
關鍵字
名稱Availability of muscle tropomyosin as a molecular marker for phylogenetic relationship of fish.
年度2017
類別研討會
摘要, .
關鍵字 tropomyosin, fish
名稱Possibility of myoglobin as a molecular marker for phylogenetic relationship of fish.
年度2017
類別研討會
摘要.
關鍵字
名稱東港產太平洋黑鮪(Thunnus orientalis)骨骼肌庫鬥蟲研究
年度2017
類別研討會
摘要
關鍵字黑鮪, 庫鬥蟲