國立臺南大學教師基本資料

基本資料
姓名 曾登裕
系所 生物科技學系
職稱 助理教授
校內分機 府城校區 789
傳真
辦公室/研究室 格致樓 C103
E-mail dytseng@mail.nutn.edu.tw
網址
專長/研究領域 動物生理學, 內分泌學, 生殖生理學
 

畢業學校國別主修學門學位修業期間
東海大學 中華民國生物學學士1989/09 ~ 1994/06
台灣大學 中華民國漁業科學碩士1994/09 ~ 1997/06
台灣大學 中華民國動物學博士1999/09 ~ 2003/06

服務機關部門 / 系所職稱服務期間
中央研究院細胞與個體生物學研究所博士後研究2005/05 ~ 2007/07
國立台南大學生物科技學系助理教授2007/08 ~ 迄今

著作
名稱Chen YN, DY Tseng, PY Ho, CM Kuo. 1999. Site of vitellogenin synthesis determined from a cDNA encoding a vitellogenin fragment in the freshwater giant prawn, Macrobrachium rosenbergii. , (SCI)
年度1999
類別期刊論文
摘要Vitellogenesis is an important part of reproductive process in crustaceans, and the process is characterized by the synthesis and accumulation of yolk protein in the developing oocytes. The yolk proteins in crustaceans mainly consist of vitellogenin (Vg) and vitellin (Vn), which are respectively present in extra-oocyte tissues and intra-oocytes. The site and the process of yolk protein synthesis in crustaceans are still controversial. The synthesis site of Vg in a crustacean species, Macrobrachium rosenbergii, is determined by immunological and immunohistochemical techniques, and molecular cloning of a cDNA encoding the primary structure of Vn in this study. The hepatopancrease is clearly shown to be the synthesis site of Vg in this species. The length of Vg mRNA was estimated as about 6 kb from Northern blotting analysis. The partial primary structure of Vg gene is presented, and the post-translational processing are further discussed. For the first time, the partial primary structure of Vg gene and the synthesis site of Vg approached by molecular cloning in crustaceans are presented.
關鍵字vitellogenin, rosenbergii, prawn
名稱Tseng DY, YN Chen, GH Kou, CF Lo, CM Kuo. 2001. Hepatopancreas is the extraovarian site of vitellogenin synthesis in black tiger shrimp, Penaeus monodon. Comparative Biochemistry and Physiology, 129A: 909-917. (SCI)
年度2001
類別期刊論文
摘要The site of yolk protein synthesis in crustaceans has long been a subject of controversy. The vitellogenin gene structure was partially reported only very recently in Macrobrachium rosenbergii, after which the hepatopancreas was confirmed as the extraovarian site of vitellogenin synthesis in that species. Ovaries are the most frequently reported as the site of yolk protein synthesis in penaeid shrimp. Using cDNA reversed-transcribed from mRNA isolated from the hepatopancreas of vitellogenic female shrimp, Penaeus monodon, we found that its deduced amino acid sequence had high identity of 48% with that from M. rosenbergii vitellogenin. A similar location of the intron in the sequenced region of genomic DNA was also found between these two species. We therefore concluded that the hepatopancreas the extraovarian site of vitellogenin synthesis in P. monodon in vivo. The partial structure of vitellogenin gene is presented in this study.
關鍵字vitellogenin, monodon, shrimp
名稱Tseng DY, YN Chen, KF Liu, GH Kou, CF Lo, CM Kuo. 2002. Hepatopancreas and ovary are sites of vitellogenin synthesis as determined from partial cDNA encoding of vitellogenin in the marine shrimp, Penaeus vannamei. , (SCI)
年度2002
類別期刊論文
摘要The site of yolk protein synthesis in crustaceans has long been a subject of controversy. The vitellogenin gene structure was partially reported only very recently in Macrobrachium rosenbergii, after which the hepatopancreas was confirmed as the extraovarian site of vitellogenin synthesis in that species. Ovaries are the most frequently reported as the site of yolk protein synthesis in penaeid shrimp. Using cDNA reversed-transcribed from mRNA isolated from the hepatopancreas of vitellogenic female shrimp, Penaeus monodon, we found that its deduced amino acid sequence had high identity of 48% with that from M. rosenbergii vitellogenin. A similar location of the intron in the sequenced region of genomic DNA was also found between these two species. We therefore concluded that the hepatopancreas the extraovarian site of vitellogenin synthesis in P. monodon in vivo. The partial structure of vitellogenin gene is presented in this study.  2001 Elsevier Science Inc. All rights reserved.
關鍵字vitellogenin, vannamei, shrimp
名稱Hsu PI, CH Liu, DY Tseng, PP Lee, W Cheng. 2006. Molecular cloning and characterization of peroxinectin, a cell adhesion molecule, from the giant freshwater prawn, Macrobrachium rosenbergii. (SCI)
年度2006
類別期刊論文
摘要Expression of peroxinectin cDNA was determined from haemocytes of giant freshwater prawn Macrobrachium rosenbergii using oligonucleotide primers and reverse transcription polymerase chain reaction (RT-PCR) based on the peroxinectin sequence of white shrimp Litopenaeus vannamei, tiger shrimp Penaeus monodon, and freshwater crayfish Pacifastacus leniusculus. The peroxinectin of M. rosenbergii was constitutively expressed. Analysis of the nucleotide sequence revealed that the cDNA clone has an open reading frame of 2,403 bp encoding a protein of 801 amino acids including a 20 amino acid signal peptide. The calculated molecular mass of the mature protein (781 amino acids) was 88.7 kDa with an estimated pI of 6.8. A putative peroxidase domain and a putative integrin-binding motif, KGD (Lys-Gly-Asp) were observed in prawn peroxinectin at the C-terminal. Sequence comparison showed that peroxinectin deduced amino acid of M. rosenbergii had an overall similarity of 62%, 64%, and 66% to that of P. leniusculus, P. monodon, and L. vannamei, respectively. Quantitative real-time PCR analysis showed that peroxinectin transcript in haemocyte of M. rosenbergii decreased significantly after 3, 6 and 12h injection with Lactococcus garvieae.
關鍵字peroxinectin, rosenbergii, prawn
名稱Liu CH, DY Tseng, CY Lai, W Cheng, CM Kuo. 2006. Molecular cloning and characterization of prophenoloxidase cDNA from haemocytes of the giant freshwater prawn, Macrobrachium rosenbergii, and its transcription in relation with the moult stage. (SCI)
年度2006
類別期刊論文
摘要Expression of prophenoloxidase (proPO) cDNA was determined from haemocytes of the giant freshwater prawn Macrobrachium rosenbergii by a reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA using oligonucleotide primers based on the proPO sequence of tiger shrimp Penaeus monodon, freshwater crayfish Pacifastacus leniusculus, green tiger shrimp Penaeus semisulcatus, kuruma shrimp Marsupenaeus japonicus, and white shrimp Litopenaeus vannamei. The proPO of M. rosenbergii was constitutively expressed. The 2,547-bp cDNA contained an open reading frame (ORF) of 2,013 bp, a 96-bp 5-untranslated region, and a 438-bp 3-untranslated region containing the poly A tail. The molecular mass of the deduced amino acid (aa) sequence (671 aa) was 76.7 kDa with an estimated pI of 7.05. It contained putative copper-binding sites, a complement-like motif (GCGWPRHM), a proteolytic activation site, and a conserved C-terminal region common to all known proPOs. However, no signal peptide sequence was detected in giant freshwater prawn proPO. Comparison of amino acid sequences showed that prawn proPO is similar to the proPO of penaeid, crayfish and lobster. Prawn proPO was only synthesised in haemocytes. The proPO transcript was significantly increased in the A stage and achieved the highest level in the B stage, and then declined sharply in the C stage and reached the lowest level in the D(2)/D(3) stage.
關鍵字prophenoloxidase, rosenbergii, prawn
名稱Tseng DY, MY Chou, YC Tseng, CD Hsiao, CJ Huang, T Kaneko, PP Hwang. 2009. Effects of Stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology, 296: R549- 57. (SCI)
年度2009
類別期刊論文
摘要Stanniocalcin (STC) formerly called hypocalcin or teleocalcin, is a 50-kDa disulfide-linked homodimeric glycoprotein that was originally identified in fish and secreted from the corpuscles of Stannius (CS). One of the main functions of STC-1 is Ca(2+) uptake inhibition; however, the mechanisms remain unknown. In the present study, we provide molecular evidence to elucidate how zebrafish STC-1 regulates Ca(2+) uptake in zebrafish embryos. In a wide variety of tissues including the kidney, brain, gill, muscle, and skin, zstc-1 was expressed. Incubating zebrafish embryos in low-Ca(2+) (0.02 mM) freshwater stimulated whole body Ca(2+) influx and zebrafish epithelial Ca(2+) channel (zECaC) mRNA expression, while downregulated zstc-1 expression. A morpholino microinjection approach was used to knockdown the zSTC-1 protein, and the results showed that the Ca(2+) content, Ca(2+) influx, and zECaC mRNA expression all increased in morphants. These data suggest that zSTC-1 negatively regulates ECaC gene expression to reduce Ca(2+) uptake in zebrafish embryos.
關鍵字Stanniocalcin, ECaC
名稱Tseng DY, PL HO, SY Huang, SC Cheng, YL Shiu, CS Chiu, CH Liu. 2009. Enhancement of immunity and disease resistance in the white shrimp, Litopenaeus vannamei, by the probiotic, Bacillus subtilis E20. (SCI)
年度2009
類別期刊論文
摘要Effects of Bacillus subtilis E20 isolated from fermented soybean on immune parameters and the disease resistance of the white shrimp (Litopenaeus vannamei) after 98 days of B. subtilis E20 feeding were evaluated in this study. Shrimp fed B. subtilis E20-containing diets at concentrations of 10(6) (E206), 10(7) (E207), and 10(8) (E208)cfu kg(-1), respectively, had significantly increased survival rates of 13.3%, 16.7%, and 20%, compared to the control (fed no probiotic) after being challenged with Vibrio alginolyticus. There were no significant differences in the total hemocyte count, respiratory burst, or superoxide dismutase glutathione peroxidase among all treatments. Shrimp fed a higher concentration of the probiotic (E208) exhibited significant increases in phenoloxidase activity, phagocytic activity, and clearance efficiency compared to control shrimp. In addition, B. subtilis E20 showed a weaker inhibitory effect against the growth of Aeromona hydrophila with around a 0.3-cm inhibitory zone, but showed no inhibitory effects against other selected pathogens, such as white shrimp pathogens: V. alginolyticus and Vibrio vulnificus. These results suggest that the increased resistance of shrimp after B. subtilis E20 consumption occurs through immune modifications, such as increases in phenoloxidase activity, phagocytic activity, and clearance efficiency against V. alginolyticus.
關鍵字vannamei, subtilis
名稱Tseng DY*, SY Li. 2011. Hydroxybenzyl Alcohols Inhibit Melanogenesis in Mouse B16 Melanoma Cells. 國立台南大學環境與生態學報, 4(1):55-64
年度2011
類別期刊論文
摘要Previous studies have shown that 4-hydroxybenzyl alcohols (4HBA) inhibited tyrosinase activity with an irreversible-like mode. In the present study, the inhibitory mechanism of 4HBA on tyrosinase activity was evaluated and the depigmenting activity of HBA derivatives was determined in both mouse B16 melanoma cells. It was demonstrated that 4HBA is not an irreversible inhibitor of tyrosinase. Among the HBA derivatives, 2HBA had the most potent inhibitory activity against melanogenesis in B16 cells. In addition, we also showed that 2HBA reduced melanogenesis of B16 cells by directing inhibiting tyrosinase activity.
關鍵字Hydroxybenzyl alcohols; inhibition; melanogenesis; tyrosinase
名稱Ding HY, TS Chang, CM Chiang, SY Li, DY Tseng*. 2011. Melanogenesis inhibition by a crude extract of Magnolia officinalis. (SCI)
年度2011
類別期刊論文
摘要In the present study, we investigated the inhibitory effect of a crude extract from Magnolia officinalis (MOE) on melanogenesis in both mouse B16 melanoma cells and zebrafish. Our results showed that MOE inhibited melanogenesis in either alpha-melanocyte stimulating hormone (alpha-MSH)- or 3-isobutyl-1-methylxanthin (IBMX)-stimulated B16 cells in a dose-dependent manner with an IC50 value of 9.3 ug/ml. In addition, MOE also inhibited cellular tyrosinase activity with an IC50 value of 13.4ug/ml while no inhibitory activity was found by MOE against cell-free tyrosinase activity. Moreover, western blotting and real time reverse-transcription polymerase chain reaction (qRT-PCR) analyses respectively confirmed that MOE downregulated levels of tyrosinase protein but not that of its mRNA inalpha-MSH-stimulated B16 cells. These results demonstrated that MOE inhibits melanogenesis of B16 cells by a post-transcriptional regulation on tyrosinase gene expression. In the other hand, when using zebrafish as a depigmenting assay system, MOE could inhibit both melanogenesis and tyrosinase activity in the in vivo model. From the present study, MOE was proven to be a good candidate as a skin-whitening agent for treatment of skin hyperpigmentation.
關鍵字Magnolia officinalis, Melanogenesis, Tyrosinase, Melanin, Inhibition
名稱Lin CH, IL Tsai, CH Su, DY Tseng, PP Hwang*. 2011. Reverse Effect of Mammalian Hypocalcemic Cortisol in Fish: Cortisol Stimulates Ca2+ Uptake via Glucocorticoid Receptor- Mediated Vitamin D3 Metabolism. (SCI)
年度2011
類別期刊論文
摘要Cortisol was reported to downregulate body-fluid Ca2+ levels in mammals but was proposed to show hypercalcemic effects in teleostean fish. Fish, unlike terrestrial vertebrates, obtain Ca2+ from the environment mainly via the gills and skin rather than by dietary means, and have to regulate the Ca2+ uptake functions to cope with fluctuating Ca2+ levels in aquatic environments. Cortisol was previously found to regulate Ca2+ uptake in fish; however, the molecular mechanism behind this is largely unclear. Zebrafish were used as a model to explore this issue. Acclimation to low-Ca2+ fresh water stimulated Ca2+ influx and expression of epithelial calcium channel (ecac), 11b-hydroxylase and the glucocorticoid receptor (gr). Exogenous cortisol increased Ca2+ influx and the expressions of ecac and hydroxysteroid 11-beta dehydrogenase 2 (hsd11b2), but downregulated 11b-hydroxylase and the gr with no effects on other Ca2+ transporters or the mineralocorticoid receptor (mr). Morpholino knockdown of the GR, but not the MR, was found to impair zebrafish Ca2+ uptake function by inhibiting the ecac expression. To further explore the regulatory mechanism of cortisol in Ca2+ uptake, the involvement of vitamin D3 was analyzed. Cortisol stimulated expressions of vitamin D-25hydroxylase (cyp27a1), cyp27a1 like (cyp27a1l), 1a-OHase (cyp27b1) at 3 dpf through GR, the first time to demonstrate the relationship between cortisol and vitamin D3 in fish. In conclusion, cortisol stimulates ecac expression to enhance Ca2+ uptake functions, and this control pathway is suggested to be mediated by the GR. Lastly, cortisol also could mediate vitamin D3 signaling to stimulate Ca2+ uptake in zebrafish.
關鍵字cortisol, Ca2+ uptake, glucocorticoid receptor
名稱Zhao J, DY Tseng, HD Lin, XT Lin*. 2011. Isolation and characterization of microsatellite markers to study population genetic diversity of White Cloud Mountain minnow (Tanichthys albonubes Lin) in wild and cultured populations. (SCI)
年度2011
類別期刊論文
摘要We developed 12 microsatellite loci for the endangered minnow species, Tanichthys albonubes, using PCR-based isolation of microsatellite arrays. These new markers were tested in 26 individuals from a wild population collected from Guangzhou in China and 26 individuals from a cultured strain. The number of alleles ranged from two to nine and the expected heterozygosity from 0.177 to 0.853. The wild population had significantly higher allelic richness than the cultured strain, with a mean allelic richness of 5.52 (range 3.69-8.64) and 3.13 (range 1.99-5.73) for the wild population and the culturedstrain, respectively. No evidence of a recent bottleneck was detected in the wild population, but it was found in the cultured strain based on the BOTTLENECK test. These primers can be used to understand the demography and to examine genetic differences between the cultured T. albonubes strains and wild populations to help determine conservation and reintroduction strategies.
關鍵字Tanichthys albonubes; Microsatellite marker; Bottleneck;
名稱Lin C-H, Su C-H, Tseng D-Y, Ding F-C, Hwang P-P (2012) Action of Vitamin D and the Receptor, VDRa, in Calcium Handling in Zebrafish (Danio rerio). (SCI)
年度2012
類別期刊論文
摘要The purpose of the present study was to use zebrafish as a model to investigate how vitamin D and its receptors interact to control Ca2+ uptake function. Low-Ca2+ fresh water stimulated Ca2+ influx and expressions of epithelial calcium channel (ecac), vitamin D-25-hydroxylase (cyp2r1), vitamin D receptor a (vdra), and vdrb in zebrafish. Exogenous vitamin D increased Ca2+ influx and expressions of ecac and 25-hydroxyvitamin D3-24-hydroxylase (cyp24a1), but downregulated 1a-OHase (cyp27b1) with no effects on other Ca2+ transporters. Morpholino oligonucleotide knockdown of VDRa, but not VDRb, was found as a consequence of calcium uptake inhibition by knockdown of ecac, and ossification of vertebrae is impaired. Taken together, vitamin D-VDRa signaling may stimulate Ca2+ uptake by upregulating ECaC in zebrafish, thereby clarifying the Ca2+-handling function of only a VDR in teleosts. Zebrafish may be useful as a model to explore the function of vitamin DVDR signaling in Ca2+ homeostasis and the related physiological processes in vertebrates.
關鍵字vitamin D, VDR, calcium
名稱Tseng DY. (2013) Complete mitochondrial genome of Onychostoma barbata (Cypriniformes, Cyprinidae). (SCI)
年度2013
類別期刊論文
摘要In this study, the complete mitogenome sequence of Onychostoma barbata has been determined using long polymerase chain reaction method. The mitogenome, consisting of 16,592 base pairs (bp), had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding, 22 transfer RNAs, 2 ribosomal RNAs genes, and a noncoding control region (CR). CR of 937 bp lengths long is located between tRNAPro and tRNAPhe. The overall base composition of O. barbata is 24.49% for T, 28.04% for C, 31.54% for A, and 15.94% for G, with a slight AT bias of 56.03%.
關鍵字Onychostoma barbata, mitogenome, Cyprinidae
名稱The complete mitochondrial genome sequence of Onychostoma alticorpus (Cypriniformes, Cyprinidae). (SCI)
年度2014
類別期刊論文
摘要We determined the complete mitochondrial genome (mitogenome) sequence of Onychostoma alticorpus, which is known as an endemic freshwater species in Taiwan, by using long polymerase chain reaction method. The total length of O. alticorpus mitogenome is 16,680 bp, consisting of 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a noncoding control region. The overall base composition of O. alticorpus is 30.88% for A, 23.57% for T, 16.56% for G and 28.99% for C, with a slight AT bias of 54.45%. Gene location and specific usage of distinct termination codon types characterize typically the vertebrate mitochondrial genome. The determination of O. alticorpus mitogenome would play an important role not only in the delineation of phylogeographic history and population genetic structure, but reflection of conservation efforts on the genetic diversity as well as population vitality.
關鍵字Cyprinidae, mitogenome, Onychostoma alticorpus
名稱Environmental and cortisol‑mediated control of Ca2+ uptake in tilapia (Oreochromis mossambicus). (SCI)
年度2016
類別期刊論文
摘要Ca2+ is a vital element for many physiological processes in vertebrates, including teleosts, which live in aquatic environments and acquire Ca2+ from their surroundings. Ionocytes within the adult gills or larval skin are critical sites for transcellular Ca2+ uptake in teleosts. The ionocytes of zebrafish were found to contain transcellular Ca2+ transporters, epithelial Ca2+ channel (ECaC), plasma membrane Ca2+-ATPase 2 (PMCA2), and Na+/ Ca2+ exchanger 1b (NCX1b), providing information about the molecular mechanism of transcellular Ca2+ transports mediated by ionocytes in fish. However, more evidence is required to establish whether or not a similar mechanism of transcellular Ca2+ transport also exists in others teleosts. In the present study, ecac, pmca2, and ncx1 were found to be expressed in the branchial ionocytes of tilapia, thereby providing further support for the mechanism of transcellular Ca2+ transport through ionocytes previously proposed for zebrafish. In addition, we also reveal that low Ca2+ water treatment of tilapia stimulates Ca2+ uptake and expression of ecac and cyp11b (the latter encodes a cortisol-synthesis enzyme). Treatment of tilapia with exogenous cortisol(20 mg/l) enhanced both Ca2+ influx and ecac expression. Therefore, increased cyp11b expression is suggested to enhance Ca2+ uptake capacity in tilapia exposed to low Ca2+ water. Furthermore, the application of cortisol receptor antagonists revealed that cortisol may regulate Ca2+ uptake through glucocorticoid and/or mineralocorticoid receptor (GR and/or MR) in tilapia. Taken together, the data suggest that cortisol may activate GR and/or MR to execute its hypercalcemic action by stimulating ecac expression in tilapia.
關鍵字ECaC · Ca2+ influx · Ionocyte · Cortisol · Tilapia
名稱Molecular characterization and expression of a novel vitellogenin gene in the freshwater giant prawn, Macrobrachium rosenbergii.
年度2007
類別研討會
摘要
關鍵字
名稱Role of Stanniocalcin-1 in regulation of the expression and function of zebrafish epithelial calcium channels
年度2008
類別研討會
摘要
關鍵字
名稱利用斑馬魚篩選抗紫外線曬傷之小分子天然物
年度2009
類別研討會
摘要
關鍵字
名稱中草藥抗神經壞死症病毒物質之研究
年度2010
類別研討會
摘要
關鍵字
名稱皮質醇調節吳郭魚鈣離子吸收作用
年度2011
類別研討會
摘要
關鍵字
名稱皮質醇調控廣鹽性吳郭魚 (Oreochromis mossambicus)鈣離子調節之機制
年度2015
類別研討會
摘要
關鍵字
名稱皮質醇經由富含肝醣細胞調控廣鹽性吳郭魚魚鰓離子調節
年度2017
類別研討會
摘要Cortisol has long been considered to be involved primarily in fish gill osmoregulation during acclimation to seawater. Scientific research and other studies have demonstrated the role that cortisol regulated calcium uptake in tilapia. We detected the location of glucocorticoid receptor (GR) mRNA via In situ hybridization. It was found that the location of glucocorticoid receptor (GR) mRNA beside gill ionocytes. These cells may be to provide energy as the glycogen-rich cells or epidermal stem cells. We found that glucocorticoid Receptor(GR) was colocalized with glycogen synthase(GS) in the glycogen-rich cells, not with Na+K+-ATPase in gill ionocytes. Cortisol affected the gene expression of glycogen synthase (GS) and glycogen phosphatase (GP). Cortisol increased glycogen phosphatase (GP) mRNA expression and decreased glycogen synthase (GS) mRNA expression in tilapia.
關鍵字cortisol, glucocorticoid receptor, glycogen-rich cells