國立臺南大學教師基本資料

基本資料
姓名 曹哲嘉
系所 生物科技學系
職稱 助理教授
校內分機 872
傳真
辦公室/研究室 C105
E-mail cctsao@mail.nutn.edu.tw
網址 http://mail.nutn.edu.tw/cctsao
專長/研究領域 遺傳學,纖毛與鞭毛,功能基因體
 

畢業學校國別主修學門學位修業期間
國立台灣大學台灣動物學學士1990-1994
國立台灣大學台灣生化科學碩士1994-1996
University of RochesterUSABiologyPh.D.1999-2006

服務機關部門 / 系所職稱服務期間
中央研究院生物化學研究所研究助理1998-1999
Yale UniversityDepartment of Molecular, Cellular, and Developmental BiologyPostdoctoral associate2006-2010
中央研究院分子生物研究所訪問學者2012/7~2012/9

著作
名稱Shang Y, Tsao, C-C, and Gorovsky MA. Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis. J. Cell Biol. 171: 1035-1044
年度2005
類別期刊論文
摘要
關鍵字
名稱Williams NE, Tsao C-C, Bowen J, Herman GE, Williams RJ, and Frankel J. The actin gene ACT1 is required for phagocytosis, motility and cell separation of Tetrahymena thermophila. Eukaryot. Cell 5: 555-567.
年度2006
類別期刊論文
摘要
關鍵字
名稱Eisen JA, Coyne RS, Wu M, Wu D, Thiagarajan M, Wortman JR, Badger JH, Ren Q, Amedeo P, Jones KM, Tallon LJ, Delcher AL, Salzberg SL, Silva JC, Haas BJ, Majoros WH, Farzad M, Carlton JM, Smith RK Jr, Garg J, Pearlman RE, Karrer KM, Sun L, Manning G, Elde NC, Turkewitz AP, Asai DJ, Wilkes DE, Wang Y, Cai H, Collins K, Stewart BA, Lee SR, Wilamowska K, Weinberg Z, Ruzzo WL, Wloga D, Gaertig J, Frankel J, Tsao C-C, Gorovsky MA, Keeling PJ, Waller RF, Patron NJ, Cherry JM, Stover NA, Krieger CJ, del Toro C, Ryder HF, Williamson SC, Barbeau RA, Hamilton EP, Orias E. Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote. PLoS Biol. 4: e286
年度2006
類別期刊論文
摘要
關鍵字
名稱Tsao C-C and Gorovsky MA. Different effects of Tetrahymena IFT172 domains on anterograde and retrograde intraflagellar transport. Mol. Biol. Cell. 19:1450-1461.
年度2008
類別期刊論文
摘要
關鍵字
名稱Tsao C-C and Gorovsky MA. Tetrahymena IFT122A is not essential for cilia assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body. J. Cell Sci. 121:428-436.
年度2008
類別期刊論文
摘要
關鍵字
名稱Craige B, Tsao C-C, Diener D, Hou Y, Lechtreck K-F, Rosenbaum LR, and Witman GB. CEP290 tethers flagellar transition zone microtubules to the membrane and regulates flagellar protein content
年度2010
類別期刊論文
摘要
關鍵字
名稱Huang, K. and Tsao, CC. Importin-β2: a key to two gates?
年度2010
類別期刊論文
摘要
關鍵字
名稱Reactive oxygen species mediate Terbufos-induced apoptosis in mouse testicular cell lines via the modulation of cell cycle and pro-apoptotic proteins.
年度2015
類別期刊論文
摘要
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名稱C.-C. Tsao and M.A. Gorovsky. Intraflagellar transport proteins required for cilia assembly in Tetrahymena. FASEB Summer Research Conference in Ciliate Molecular Biology. Tuscana, Italy.
年度2005
類別研討會
摘要
關鍵字
名稱C.-C. Tsao and M.A. Gorovsky. The IFT Complex A Protein, IFT122, is not essential for ciliary assembly but is required for retrograde (tip to cell body) IFT. FASEB Summer Research Conference in Biology of Cilia and Flagella.
年度2007
類別研討會
摘要
關鍵字
名稱C.-C. Tsao, C. R. Wood, and J.L. Rosenbaum. The small GTPase Ran and nuclear transport adapter protein importin-are present in the flagella in Chlamydomonas reinhardtii. The 48th Annual Meeting of the American Society for Cell Biology. San Francisco, CA.
年度2008
類別研討會
摘要
關鍵字
名稱B. Craige, C.-C. Tsao, D.R. Diener, Y. Hou, K. Lechtreck, J.L. Rosenbaum, and G.B. Witman. Chlamydomonas CEP290 is a dynamic component of the transition zone required for normal intraflagellar transport and flagellar assembly. Keystone Symposium on “Cilia, Signaling, and Human Disease.” Monterey, CA
年度2010
類別研討會
摘要
關鍵字
名稱B. Craige, C.-C. Tsao, D. Diener, Y. Hou, K. Lechtreck, J.L. Rosenbaum, and G.B. Witman. CEP290 is a dynamic transition zone protein required for tethering the membrane to the transition zone microtubules, normal intraflagellar transport (IFT), and flagellar assembly. The 14th International Conference on the Cell and Molecular Biology of Chlamydomonas. Norton, MA.
年度2010
類別研討會
摘要Mutations in human CEP290 cause cilia-related disorders that range in severity from isolated blindness to perinatal lethality. The Chlamydomonas CEP290 homologue, encoded by POC3, is highly conserved (BLAST E 5e-27). A PCR screen of a library of Chlamydomonas insertional mutants identified a strain in which all but the first 2-4 exons of POC3 are deleted. The mutant cells have defects in flagellar assembly and motility. The mutant phenotype co-segregated with the deletion, and transformation of the mutant with genomic DNA encoding wild-type or HA-tagged CEP290 rescued the mutant phenotype. Immunofluorescence (IF) and immuno-EM localization indicated that CEP290 is located in the transition zone between the outer doublets and the membrane. Ultrastructural analysis revealed defects in the connections between the outer doublets of the transition zone and the flagellar membrane in mutant cells, and the detergent-resistant flagellar membrane that remains attached to wild-type transition zones after detergent extraction was lost in detergent-extracted mutant cells. Some flagella display bulges filled with electron dense material; IF indicated that the bulges contain IFT proteins. Western blots of wild-type and mutant flagella revealed that the mutant flagella contain increased levels of IFT complex B proteins and BBS4, and decreased levels of the IFT complex A subunit IFT139 and polycystin-2. Dikaryon rescue experiments revealed that CEP290 is dynamic and can incorporate into pre-assembled wild-type or CEP290-deficient transition zones. The results indicate that CEP290 is required to tether the flagellar membrane to the transition zone microtubules, and functions in establishing the normal ratio of IFT complex A to B in flagella.
關鍵字
名稱Che-Chia Tsao. 連結教室與研究室:從單胞藻與纖毛的生長組裝談起 Chlamydomonas and cilia assembly: a trial to bridge undergraduate education and research laboratory. 2011生命科學的跨領域整合與應用研討會. 台灣,宜蘭.
年度2011
類別研討會
摘要Chlamydomonas is a unicellular diflagellated green microalga that is widely used in biochemical, cell biological, and genetic researches. It is easy to culture and inexpensive to maintain in the laboratory setting. Furthermore, with sequenced genome, Chlamydomonas is amenable to isolate mutants and identify the affected gene, which allows functional genomic analysis through insertional mutagenesis, phenotypic screen, and molecular genetic analysis. A preliminary implementation of this system in the sophomore Genetics Laboratory course at Department of Biological Sciences and Technology in National University of Tainan during fall semester of 2010 shows that using a cell-wall defect strain and a revised procedure to transform Chlamydomonas by glass beads method, undergraduate students can perform fairly well in generating insertional mutants. Among tens of thousands of transformants, a dozen of cell motility mutants were obtained throughout the course. Along with other experimental modules, such as strain culturing and handling, phenotype observation, nucleic acid preparation, polymerase chain reaction, and DNA gel electrophoresis, students can learn both basic concepts and hands-on experience in functional genomics, which lays down the foundation for further in-depth research. (This program is supported by College of Environmental Sciences and Ecology and National University of Tainan.)
關鍵字
名稱Preliminary characterization of a protofilamant-ribbon gene in Tetrahymena thermophila
年度2013
類別研討會
摘要
關鍵字
名稱Chlamydomonas MKS1 is required for fully assembly of flagella beyond the transition zone.
年度2014
類別研討會
摘要
關鍵字