國立臺南大學教師基本資料

基本資料
姓名 曹哲嘉
系所 生物科技學系
職稱 助理教授
校內分機 872
傳真
辦公室/研究室 C105
E-mail cctsao@mail.nutn.edu.tw
網址 http://mail.nutn.edu.tw/cctsao
專長/研究領域 遺傳學,纖毛與鞭毛,功能基因體
 

畢業學校國別主修學門學位修業期間
國立台灣大學台灣動物學學士1990-1994
國立台灣大學台灣生化科學碩士1994-1996
University of RochesterUSABiologyPh.D.1999-2006

服務機關部門 / 系所職稱服務期間
中央研究院生物化學研究所研究助理1998-1999
Yale UniversityDepartment of Molecular, Cellular, and Developmental BiologyPostdoctoral associate2006-2010
中央研究院分子生物研究所訪問學者2012/7~2012/9

著作
名稱Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis
年度2005
類別期刊論文
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名稱The actin gene ACT1 is required for phagocytosis, motility and cell separation of Tetrahymena thermophila
年度2006
類別期刊論文
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名稱Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote
年度2006
類別期刊論文
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名稱Different effects of Tetrahymena IFT172 domains on anterograde and retrograde intraflagellar transport
年度2008
類別期刊論文
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名稱Tetrahymena IFT122A is not essential for cilia assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body
年度2008
類別期刊論文
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名稱Craige B, Tsao C-C, Diener D, Hou Y, Lechtreck K-F, Rosenbaum LR, and Witman GB. CEP290 tethers flagellar transition zone microtubules to the membrane and regulates flagellar protein content
年度2010
類別期刊論文
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名稱Huang, K. and Tsao, CC. Importin-β2: a key to two gates?
年度2010
類別期刊論文
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名稱Reactive oxygen species mediate Terbufos-induced apoptosis in mouse testicular cell lines via the modulation of cell cycle and pro-apoptotic proteins.
年度2015
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名稱Biological Protective Effects Against Vibrio Infections in Grouper Larvae Using the Strombidium sp. NTOU1, a Marine Ciliate Amenable for Scaled-Up Culture and With an Excellent Bacteriovorous Ability
年度2019
類別期刊論文
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名稱連結教室與研究室:從單胞藻與纖毛的生長組裝談起
年度2011
類別會議論文
摘要Chlamydomonas is a unicellular diflagellated green microalga that is widely used in biochemical, cell biological, and genetic researches. It is easy to culture and inexpensive to maintain in the laboratory setting. Furthermore, with sequenced genome, Chlamydomonas is amenable to isolate mutants and identify the affected gene, which allows functional genomic analysis through insertional mutagenesis, phenotypic screen, and molecular genetic analysis. A preliminary implementation of this system in the sophomore Genetics Laboratory course at Department of Biological Sciences and Technology in National University of Tainan during fall semester of 2010 shows that using a cell-wall defect strain and a revised procedure to transform Chlamydomonas by glass beads method, undergraduate students can perform fairly well in generating insertional mutants. Among tens of thousands of transformants, a dozen of cell motility mutants were obtained throughout the course. Along with other experimental modules, such as strain culturing and handling, phenotype observation, nucleic acid preparation, polymerase chain reaction, and DNA gel electrophoresis, students can learn both basic concepts and hands-on experience in functional genomics, which lays down the foundation for further in-depth research. (This program is supported by College of Environmental Sciences and Ecology and National University of Tainan.)
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名稱Preliminary characterization of a protofilamant-ribbon gene in Tetrahymena thermophila
年度2013
類別會議論文
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名稱Chlamydomonas MKS1 is required for fully assembly of flagella beyond the transition zone.
年度2014
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名稱Chlamydomonas IC140 is essential for the flagellar assembly while B9D1 affects the level of intraflagellar transport proteins and is dispensable for structural integrity of the transition zone
年度2016
類別會議論文
摘要We characterized a Chlamydomonas mutant without flagella obtained from insertional mutagenesis. In this Lu3 strain, the ultrastructure of its basal body and transition zone showed no defect, but the axoneme terminated abruptly distal to the transition zone. A 60-kb chromosomal region containing IC140 and B9D1 was deleted in the Lu3 mutant, and re-transformation of both genes was sufficient to restore the flagellar assembly and motility. Reintroducing IC140, encoding a subunit of the I1 flagellar inner arm dynein complex, could partially rescue the flagellar formation and motility, but the resulted b9d1 strain exhibited incomplete flagellation and flagellar length heterogeneity. Unexpectedly, the ic140 strain, derived from restoration of the transition zone protein B9d1 in the Lu3 background, failed to assemble any flagella. We found the level of intraflagellar transport (IFT) proteins markedly decreased in the strains without B9D1 but not in the ic140 strain. Our results indicated that B9D1 is not essential for the structural integrity of the transition zone, but its disruption lowered the protein level of IFT and compromised the flagellar biogenesis. We also uncovered a novel role of IC140 that it is required not only for the flagellar motility but also for the axonemal assembly through an unidentified mechanism.
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